Reviewed, UniProtKB/Swiss-Prot P57290 (AKH_BUCAI)

Names and origin

Protein names

Recommended name:
    Bifunctional aspartokinase/homoserine dehydrogenase
      Short name=AK-HD
Including the following 2 domains:
    1- Recommended name:
            Aspartokinase
              EC=2.7.2.4
    2- Recommended name:
            Homoserine dehydrogenase
              EC=1.1.1.3

Gene names

Name:	thrA
Ordered Locus Names:	BU194

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (Acyrthosiphon pisum symbiotic bacterium) [Complete proteome] [HAMAP]

Taxonomic identifier

118099 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	816 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-homoserine + NAD(P)(+) = L-aspartate 4-semialdehyde + NAD(P)H. ATP + L-aspartate = ADP + 4-phospho-L-aspartate.
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; tetrahydrodipicolinate from L-aspartate: step 1/4. Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 1/3. Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 3/3. Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 1/5. Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 3/5.
Subunit structure	Homotetramer By similarity.
Sequence similarities	In the N-terminal section; belongs to the aspartokinase family. In the C-terminal section; belongs to the homoserine dehydrogenase family. Contains 2 ACT domains.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Threonine biosynthesis
Ligand	NADP
Molecular function	Kinase Oxidoreductase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 816	816	Bifunctional aspartokinase/homoserine dehydrogenase
Regions
	Domain	398 – 468	71	ACT
	Nucleotide binding	472 – 479	8	NADP Potential
	Region	1 – 250	250	Aspartokinase By similarity
	Region	251 – 471	221	Interface By similarity
	Region	472 – 816	345	Homoserine dehydrogenase By similarity

Sequences

	Sequence		Length	Mass (Da)
	P57290-1 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: CEACE5C565465884	FASTA	816	91,809
	10 20 30 40 50 60 MKLLKFGGTS LANAEKFLSV SSIIEENTQT DQIAVVLSAP AKITNYLVKI IENTIKNNQI 70 80 90 100 110 120 LETVHLAENI FMQLINNFLN IQSNFPHKEI EKIIKKEFNE LKNIIQGILL LKQCPDNIRA 130 140 150 160 170 180 IIISRGEILS VFIMKSILQS KNYNVTIINP VKNLVAIGDN YLDSTVDISE SKKNIQNMNI 190 200 210 220 230 240 NQSNIILMAG FIAGNKDKKL VVLGRNGSDY SAAVLAACLD ANCCEIWTDV DGVFTSDPRK 250 260 270 280 290 300 VPNARLLKSI SYQEAMELSY FGAKVLHPRT IEPIAQFKIP CLIKNTNNVK SIGTLICEQN 310 320 330 340 350 360 CSEKDFLKGV THLDEIAMFN ISGPHIKDVG SVISRIFTMM SRGNIKILLI TQSSSENKIN 370 380 390 400 410 420 FCVYEHDIYK ILYLFNKEFQ LELKDGLLNP FKIKKNLSIL SIVGSNIYKK HNIASKIFSV 430 440 450 460 470 480 LGALKINVIA ISQGSSKHSI SLVIKKENIL KAVQHVHNTL FFNKKTIHMF LIGIGGIGST 490 500 510 520 530 540 LLNQILKQKQ FLDKKNIEIK ICAIANSKKI LLLDNTNDLS NWKNDFKKST QKFNLELLNN 550 560 570 580 590 600 LIKNNHFSNS VIVDCTSSQL LSEQYVNFID NNFHVVTSNK KANTSEWNYY KKIRKSVAQT 610 620 630 640 650 660 GKKFLYETNV GAGLPVIETL QNLFKSGDNL ICFKGILSGS LSFIFGRLEE GILLSQATRE 670 680 690 700 710 720 AKELGFTEPN PCDDLSGIDV ARKLLILARE SGYNIELKDI KIEPLLPNNF KIYEDTDKFL 730 740 750 760 770 780 LKLKELDVYF SNKIKQALNV GNVLRFVATI EQKRQFFIKL EEVNINNPLY KVKNGENALA 790 800 810 FYTNYYQPIP LVLRGYGAGN NVTASGVFSD LLRTLS

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tokyo 1998.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000003 Genomic DNA. Translation: BAB12911.1.
RefSeq	NP_240025.1.
3D structure databases
HSSP	HSSP built from PDB template 1EBF based on UniProtKB P31116.
ModBase	Search...
Genome annotation databases
GeneID	1109637.
GenomeReviews	Gene locus BU194 in contig BA000003_GR.
KEGG	buc:BU194.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P57290.
Enzyme and pathway databases
BioCyc	BSP107806:BU194-MON.
Family and domain databases
InterPro	IPR002912. ACT_bd. IPR001048. Asp/Glu/Uridylate_kinase. IPR005106. Asp/hSer_DHase_NAD-bd. IPR001341. Asp_kin_reg. IPR011147. bifunc_aspartokin/hSer_DHase. IPR001342. hSer_DHase_cat. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00696. AA_kinase. 1 hit. PF01842. ACT. 1 hit. PF00742. Homoserine_dh. 1 hit. PF03447. NAD_binding_3. 1 hit. [Graphical view]
PIRSF	PIRSF000727. ThrA. 1 hit.
TIGRFAMs	TIGR00657. asp_kinases. 1 hit.
PROSITE	PS00324. ASPARTOKINASE. 1 hit. PS01042. HOMOSER_DHGENASE. 1 hit. [Graphical view]
ProDom	P57290. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

AKH_BUCAI

Accession

Primary (citable) accession number: P57290

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	July 22, 2008
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)