Reviewed, UniProtKB/Swiss-Prot P59510 (ATS20_HUMAN)

Names and origin

Protein names

Recommended name:
    ADAMTS-20
    EC=3.4.24.-
Alternative name(s):
    A disintegrin and metalloproteinase with thrombospondin motifs 20
      Short name(s)=ADAM-TS 20, ADAM-TS20

Gene names

Name:

ADAMTS20

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1911 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May play a role in tissue-remodeling process occurring in both normal and pathological conditions.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subcellular location	Secreted › extracellular space › extracellular matrixBy similarity.
Tissue specificity	Very sparingly expressed, although is detected at low levels in testis, prostate, ovary, heart, placenta, lung and pancreas. Overexpressed in several brain, colon and breast carcinomas.
Post-translational modification	The precursor is cleaved by a furin endopeptidase By similarity.
Sequence similarities	Contains 1 disintegrin domain. Contains 1 GON domain. Contains 1 peptidase M12B domain. Contains 15 TSP type-1 domains.

Ontologies

Keywords
Cellular component	Extracellular matrix Secreted
Coding sequence diversity	Alternative splicing
Domain	Repeat Signal
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
PTM	Cleavage on pair of basic residues Glycoprotein Zymogen
Gene Ontology (GO)
None. [Check GOA]

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P59510-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P59510-2)

The sequence of this isoform differs from the canonical sequence as follows:
     683-693: THDICVQGQCM → SYNIDCNCVLK
     1430-1504: CSASCGKGRK...QQRDVYCRLK → EDLKVKLLPQ...GDLKYYKNSL
     1505-1911: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Propeptide	22 – 253	232	By similarity
	Chain	254 – 1911	1658	ADAMTS-20
Regions
	Domain	259 – 467	209	Peptidase M12B
	Domain	468 – 555	88	Disintegrin
	Domain	556 – 611	56	TSP type-1 1
	Domain	847 – 905	59	TSP type-1 2
	Domain	906 – 962	57	TSP type-1 3
	Domain	967 – 1024	58	TSP type-1 4
	Domain	1025 – 1074	50	TSP type-1 5
	Domain	1077 – 1136	60	TSP type-1 6
	Domain	1153 – 1207	55	TSP type-1 7
	Domain	1208 – 1265	58	TSP type-1 8
	Domain	1305 – 1357	53	TSP type-1 9
	Domain	1359 – 1417	59	TSP type-1 10
	Domain	1418 – 1476	59	TSP type-1 11
	Domain	1477 – 1532	56	TSP type-1 12
	Domain	1536 – 1589	54	TSP type-1 13
	Domain	1590 – 1653	64	TSP type-1 14
	Domain	1655 – 1711	57	TSP type-1 15
	Domain	1712 – 1911	200	GON
	Region	724 – 846	123	Spacer
	Compositional bias	612 – 723	112	Cys-rich
Sites
	Active site	404	1	By similarity
	Metal binding	403	1	Zinc (catalytic) By similarity
	Metal binding	407	1	Zinc (catalytic) By similarity
	Metal binding	413	1	Zinc (catalytic) By similarity
Amino acid modifications
	Glycosylation	92	1	N-linked (GlcNAc...) Potential
	Glycosylation	191	1	N-linked (GlcNAc...) Potential
	Glycosylation	445	1	N-linked (GlcNAc...) Potential
	Glycosylation	702	1	N-linked (GlcNAc...) Potential
	Glycosylation	717	1	N-linked (GlcNAc...) Potential
	Glycosylation	728	1	N-linked (GlcNAc...) Potential
	Glycosylation	809	1	N-linked (GlcNAc...) Potential
	Glycosylation	870	1	N-linked (GlcNAc...) Potential
	Glycosylation	1062	1	N-linked (GlcNAc...) Potential
	Glycosylation	1457	1	N-linked (GlcNAc...) Potential
	Glycosylation	1543	1	N-linked (GlcNAc...) Potential
	Glycosylation	1573	1	N-linked (GlcNAc...) Potential
	Glycosylation	1764	1	N-linked (GlcNAc...) Potential
	Glycosylation	1782	1	N-linked (GlcNAc...) Potential
	Glycosylation	1853	1	N-linked (GlcNAc...) Potential
	Disulfide bond	381 ↔ 462		By similarity
	Disulfide bond	419 ↔ 446		By similarity
	Disulfide bond	568 ↔ 605		By similarity
	Disulfide bond	572 ↔ 610		By similarity
	Disulfide bond	583 ↔ 595		By similarity
Natural variations
	Alternative sequence	683 – 693	11	THDICVQGQCM → SYNIDCNCVLK in isoform 2.
	Alternative sequence	1430 – 1504	75	CSASC…YCRLK → EDLKVKLLPQRTIILWELMK NIFCHGKHSHMYLINVVTDH LLYPRHCDPETIETYFLSLW SLQFTWGDLKYYKNSL in isoform 2.
	Alternative sequence	1505 – 1911	407	Missing in isoform 2.
Experimental info
	Sequence conflict	152	1	T → V in CAD56159 and CAD56160. Ref.2
	Sequence conflict	360	1	E → Y in CAD56159 and CAD56160. Ref.2
	Sequence conflict	371	1	T → M in CAD56159 and CAD56160. Ref.2
	Sequence conflict	456	1	D → E in CAD56159 and CAD56160. Ref.2
	Sequence conflict	648	1	I → V in CAD56159 and CAD56160. Ref.2
	Sequence conflict	792 – 793	2	Missing in CAD56159 and CAD56160. Ref.2
	Sequence conflict	818 – 821	4	LILQ → ILIE in CAD56159 and CAD56160. Ref.2
	Sequence conflict	908 – 913	6	GMLLAK → WHVIG in CAD56159. Ref.2
	Sequence conflict	1316	1	S → Q in CAD56159 and CAD56160. Ref.2
	Sequence conflict	1328	1	T → R in CAD56159 and CAD56160. Ref.2
	Sequence conflict	1817	1	T → A in CAD56159. Ref.2
	Sequence conflict	1882	1	E → Q in CAD56159. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified April 4, 2003. Version 1. Checksum: CF592E220D32B250	FASTA	1,911	214,656
	10 20 30 40 50 60 MWVAKWLTGL LYHLSLFITR SWEVDFHPRQ EALVRTLTSY EVVIPERVNE FGEVFPQSHH 70 80 90 100 110 120 FSRQKRSSEA LEPMPFRTHY RFTAYGQLFQ LNLTADASFL AAGYTEVHLG TPERGAWESD 130 140 150 160 170 180 AGPSDLRHCF YRGQVNSQED YKAVVSLCGG LTGTFKGQNG EYFLEPIMKA DGNEYEDGHN 190 200 210 220 230 240 KPHLIYRQDL NNSFLQTLKY CSVSESQIKE TSLPFHTYSN MNEDLNVMKE RVLGHTSKNV 250 260 270 280 290 300 PLKDERRHSR KKRLISYPRY IEIMVTADAK VVSAHGSNLQ NYILTLMSIV ATIYKDPSIG 310 320 330 340 350 360 NLIHIVVVKL VMIHREEEGP VINFDGATTL KNFCSWQQTQ NDLDDVHPSH HDTAVLITRE 370 380 390 400 410 420 DICSSKEKCN TLGLSYLGTI CDPLQSCFIN EEKGLISAFT IAHELGHTLG VQHDDNPRCK 430 440 450 460 470 480 EMKVTKYHVM APALSFHMSP WSWSNCSRKY VTEFLDTGYG ECLLDKPDEE IYNLPSELPG 490 500 510 520 530 540 SRYDGNKQCE LAFGPGSQMC PHINICMHLW CTSTEKLHKG CFTQHVPPAD GTDCGPGMHC 550 560 570 580 590 600 RHGLCVNKET ETRPVNGEWG PWEPYSSCSR TCGGGIESAT RRCNRPEPRN GGNYCVGRRM 610 620 630 640 650 660 KFRSCNTDSC PKGTQDFREK QCSDFNGKHL DISGIPSNVR WLPRYSGIGT KDRCKLYCQV 670 680 690 700 710 720 AGTNYFYLLK DMVEDGTPCG TETHDICVQG QCMAAGCDHV LNSSAKIDKC GVCGGDNSSC 730 740 750 760 770 780 KTITGVFNSS HYGYNVVVKI PAGATNVDIR QYSYSGQPDD SYLALSDAEG NFLFNGNFLL 790 800 810 820 830 840 STSKKEINVQ GTRTVIEYSG SNNAVERINS TNRQEKELIL QVLCVGNLYN PDVHYSFNIP 850 860 870 880 890 900 LEERSDMFTW DPYGPWEGCT KMCQGLQRRN ITCIHKSDHS VVSDKECDHL PLPSFVTQSC 910 920 930 940 950 960 NTDCELRGML LAKKSECSSQ CGQGYRTLDI HCMKYSIHEG QTVQVDDHYC GDQLKPPTQE 970 980 990 1000 1010 1020 LCHGNCVFTR WHYSEWSQCS RSCGGGERSR ESYCMNNFGH RLADNECQEL SRVTRENCNE 1030 1040 1050 1060 1070 1080 FSCPSWAASE WSECLVTCGK GTKQRQVWCQ LNVDHLSDGF CNSSTKPESL SPCELHTCAS 1090 1100 1110 1120 1130 1140 WQVGPWGPCT TTCGHGYQMR DVKCVNELAS AVLEDTECHE ASRPSDRQSC VLTPCSFISK 1150 1160 1170 1180 1190 1200 LETALLPTVL IKKMAQWRHG SWTPCSVSCG RGTQARYVSC RDALDRIADE SYCAHLPRPA 1210 1220 1230 1240 1250 1260 EIWDCFTPCG EWQAGDWSPC SASCGHGKTT RQVLCMNYHQ PIDENYCDPE VRPLMEQECS 1270 1280 1290 1300 1310 1320 LAACPPAHSH FPSSPVQPSY YLSTNLPLTQ KLEDNENQVV HPSVRGNQWR TGPWGSCSSS 1330 1340 1350 1360 1370 1380 CSGGLQHTAV VCQDENGQSA SYCDAASKPP ELQQCGPGPC PQWNYGNWGE CSQTCGGGIK 1390 1400 1410 1420 1430 1440 SRLVICQFPN GQILEDHNCE IVNKPPSVIQ CHMHACPADV SWHQEPWTSC SASCGKGRKY 1450 1460 1470 1480 1490 1500 REVFCIDQFQ RKLEDTNCSQ VQKPPTHKAC RSVRCPSWKA NSWNECSVTC GSGVQQRDVY 1510 1520 1530 1540 1550 1560 CRLKGVGQVV EEMCDQSTRP CSQRRCWSQD CVQHKGMERG RLNCSTSCER KDSHQRMECT 1570 1580 1590 1600 1610 1620 DNQIRQVNEI VYNSSTISLT SKNCRNPPCN YIVVTADSSQ CANNCGFSYR QRITYCTEIP 1630 1640 1650 1660 1670 1680 STKKHKLHRL RPIVYQECPV VPSSQVYQCI NSCLHLATWK VGKWSKCSVT CGIGIMKRQV 1690 1700 1710 1720 1730 1740 KCITKHGLSS DLCLNHLKPG AQKKCYANDC KSFTTCKEIQ VKNHIRKDGD YYLNIKGRII 1750 1760 1770 1780 1790 1800 KIYCADMYLE NPKEYLTLVQ GEENFSEVYG FRLKNPYQCP FNGSRREDCE CDNGHLAAGY 1810 1820 1830 1840 1850 1860 TVFSKIRIDL TSMQIKTTDL LFSKTIFGNA VPFATAGDCY SAFRCPQGQF SINLSGTGMK 1870 1880 1890 1900 1910 ISSTAKWLTQ GSYTSVSIRR SEDGTRFFGK CGGYCGKCLP HMTTGLPIQV I
	Isoform 2 [UniParc]. Checksum: CB4E32CA7FEC5E31		1,505	169,441
	10 20 30 40 50 60 MWVAKWLTGL LYHLSLFITR SWEVDFHPRQ EALVRTLTSY EVVIPERVNE FGEVFPQSHH 70 80 90 100 110 120 FSRQKRSSEA LEPMPFRTHY RFTAYGQLFQ LNLTADASFL AAGYTEVHLG TPERGAWESD 130 140 150 160 170 180 AGPSDLRHCF YRGQVNSQED YKAVVSLCGG LTGTFKGQNG EYFLEPIMKA DGNEYEDGHN 190 200 210 220 230 240 KPHLIYRQDL NNSFLQTLKY CSVSESQIKE TSLPFHTYSN MNEDLNVMKE RVLGHTSKNV 250 260 270 280 290 300 PLKDERRHSR KKRLISYPRY IEIMVTADAK VVSAHGSNLQ NYILTLMSIV ATIYKDPSIG 310 320 330 340 350 360 NLIHIVVVKL VMIHREEEGP VINFDGATTL KNFCSWQQTQ NDLDDVHPSH HDTAVLITRE 370 380 390 400 410 420 DICSSKEKCN TLGLSYLGTI CDPLQSCFIN EEKGLISAFT IAHELGHTLG VQHDDNPRCK 430 440 450 460 470 480 EMKVTKYHVM APALSFHMSP WSWSNCSRKY VTEFLDTGYG ECLLDKPDEE IYNLPSELPG 490 500 510 520 530 540 SRYDGNKQCE LAFGPGSQMC PHINICMHLW CTSTEKLHKG CFTQHVPPAD GTDCGPGMHC 550 560 570 580 590 600 RHGLCVNKET ETRPVNGEWG PWEPYSSCSR TCGGGIESAT RRCNRPEPRN GGNYCVGRRM 610 620 630 640 650 660 KFRSCNTDSC PKGTQDFREK QCSDFNGKHL DISGIPSNVR WLPRYSGIGT KDRCKLYCQV 670 680 690 700 710 720 AGTNYFYLLK DMVEDGTPCG TESYNIDCNC VLKAAGCDHV LNSSAKIDKC GVCGGDNSSC 730 740 750 760 770 780 KTITGVFNSS HYGYNVVVKI PAGATNVDIR QYSYSGQPDD SYLALSDAEG NFLFNGNFLL 790 800 810 820 830 840 STSKKEINVQ GTRTVIEYSG SNNAVERINS TNRQEKELIL QVLCVGNLYN PDVHYSFNIP 850 860 870 880 890 900 LEERSDMFTW DPYGPWEGCT KMCQGLQRRN ITCIHKSDHS VVSDKECDHL PLPSFVTQSC 910 920 930 940 950 960 NTDCELRGML LAKKSECSSQ CGQGYRTLDI HCMKYSIHEG QTVQVDDHYC GDQLKPPTQE 970 980 990 1000 1010 1020 LCHGNCVFTR WHYSEWSQCS RSCGGGERSR ESYCMNNFGH RLADNECQEL SRVTRENCNE 1030 1040 1050 1060 1070 1080 FSCPSWAASE WSECLVTCGK GTKQRQVWCQ LNVDHLSDGF CNSSTKPESL SPCELHTCAS 1090 1100 1110 1120 1130 1140 WQVGPWGPCT TTCGHGYQMR DVKCVNELAS AVLEDTECHE ASRPSDRQSC VLTPCSFISK 1150 1160 1170 1180 1190 1200 LETALLPTVL IKKMAQWRHG SWTPCSVSCG RGTQARYVSC RDALDRIADE SYCAHLPRPA 1210 1220 1230 1240 1250 1260 EIWDCFTPCG EWQAGDWSPC SASCGHGKTT RQVLCMNYHQ PIDENYCDPE VRPLMEQECS 1270 1280 1290 1300 1310 1320 LAACPPAHSH FPSSPVQPSY YLSTNLPLTQ KLEDNENQVV HPSVRGNQWR TGPWGSCSSS 1330 1340 1350 1360 1370 1380 CSGGLQHTAV VCQDENGQSA SYCDAASKPP ELQQCGPGPC PQWNYGNWGE CSQTCGGGIK 1390 1400 1410 1420 1430 1440 SRLVICQFPN GQILEDHNCE IVNKPPSVIQ CHMHACPADV SWHQEPWTSE DLKVKLLPQR 1450 1460 1470 1480 1490 1500 TIILWELMKN IFCHGKHSHM YLINVVTDHL LYPRHCDPET IETYFLSLWS LQFTWGDLKY YKNSL

References

[1]	"Characterization of ADAMTS-9 and ADAMTS-20 as a distinct ADAMTS subfamily related to Caenorhabditis elegans GON-1." Somerville R.P., Longpre J.-M., Jungers K.A., Engle J.M., Ross M., Evanko S., Wight T.N., Leduc R., Apte S.S. J. Biol. Chem. 278:9503-9513(2003) [PubMed: 12514189] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Identification and characterization of ADAMTS-20 defines a novel subfamily of metalloproteinases-disintegrins with multiple thrombospondin-1 repeats and a unique GON domain." Llamazares M., Cal S., Quesada V., Lopez-Otin C. J. Biol. Chem. 278:13382-13389(2003) [PubMed: 12562771] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2). Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF488804 mRNA. Translation: AAO15766.1. AJ515153 mRNA. Translation: CAD56159.3. AJ515154 mRNA. Translation: CAD56160.2.
RefSeq	NP_079279.3.
UniGene	Hs.287554
3D structure databases
HSSP	HSSP built from PDB template 1LSL based on UniProtKB P07996.
ModBase	Search...
Protein family/group databases
MEROPS	M12.246.
Genome annotation databases
Ensembl	ENSG00000173157. Homo sapiens. [Contig view]
GeneID	80070.
KEGG	hsa:80070.
Organism-specific databases
H-InvDB	HIX0010556.
HGNC	HGNC:17178. ADAMTS20.
MIM	611681. gene.
PharmGKB	PA134901626.
GenAtlas	Search...
GeneCards	Search...
GeneLynx	Search...
Phylogenomic databases
HOVERGEN	P59510.
Gene expression databases
ArrayExpress	P59510.
CleanEx	HS_ADAMTS20.
GermOnline	ENSG00000173157. Homo sapiens.
Family and domain databases
InterPro	IPR010294. ADAM_spacer1. IPR001762. Blood-coag_inhib_Disintegrin. IPR012314. Pept_M12B_GON-ADAMTSs. IPR006025. Pept_M_Zn_BS. IPR001590. Peptidase_M12B. IPR013273. Peptidase_M12B_ADAM-TS. IPR002870. Peptidase_M12B_N. IPR000884. TSP1. [Graphical view]
Pfam	PF05986. ADAM_spacer1. 1 hit. PF08685. GON. 1 hit. PF01562. Pep_M12B_propep. 1 hit. PF01421. Reprolysin. 1 hit. PF00090. TSP_1. 10 hits. [Graphical view]
PRINTS