Actin - Saccharomyces cerevisiae (Baker's yeast)

Names and origin

Protein names

Recommended name:
Actin

Gene names

Name:	ACT1
Synonyms:	ABY1, END7
Ordered Locus Names:	YFL039C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length	375 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Subunit structure	Component of the INO80 complex. Component of the SWR1 complex. Component of the NuA4 complex.
Subcellular location	Cytoplasm › cytoskeleton.
Sequence similarities	Belongs to the actin family.

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Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton
Ligand	ATP-binding Nucleotide-binding
Molecular function	Structural protein
PTM	Acetylation Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	DNA repair Inferred from direct assay. Source: SGD budding cell isotropic bud growth Traceable author statement. Source: SGD cell wall organization and biogenesis Traceable author statement. Source: SGD chronological cell aging Inferred from mutant phenotype. Source: SGD contractile ring contraction involved in cytokinesis Inferred from direct assay. Source: SGD endocytosis Traceable author statement. Source: SGD establishment of mitotic spindle orientation Traceable author statement. Source: SGD exocytosis Traceable author statement. Source: SGD histone acetylation Inferred from direct assay. Source: SGD mitochondrion inheritance Traceable author statement. Source: SGD regulation of transcription from RNA polymerase II promoter Inferred from direct assay. Source: SGD response to osmotic stress Traceable author statement. Source: SGD response to oxidative stress Inferred from genetic interaction. Source: SGD vacuole inheritance Inferred from genetic interaction. Source: SGD vesicle transport along actin filament Traceable author statement. Source: SGD
Cellular component	INO80 complex Inferred from physical interaction. Source: SGD NuA4 histone acetyltransferase complex Inferred from physical interaction. Source: SGD SWR1 complex Inferred from direct assay. Source: SGD actin cable Traceable author statement. Source: SGD actin cortical patch Traceable author statement. Source: SGD cellular bud neck contractile ring Traceable author statement. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct
FMP27	Q06179	1	EBI-2169,EBI-32551
LAS17	Q12446	2	EBI-2169,EBI-10022
MGS1	P40151	1	EBI-2169,EBI-29152
RVS167	P39743	2	EBI-2169,EBI-14500
SAC6	P32599	1	EBI-2169,EBI-6931
SWR1	Q05471	1	EBI-2169,EBI-22102

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Molecule processing

Chain

1 – 375

375

Actin

Amino acid modifications

Modified residue

1

N-acetylmethionine

Modified residue

199

1

Phosphoserine

Modified residue

201

1

Phosphoserine

Modified residue

323

1

Phosphoserine

Modified residue

324

1

Phosphoserine

Natural variations

Natural variant

143

1

Y → F in strain: CBS 1907.

Experimental info

Sequence conflict

178

1

I → L in CAA24598. Ref.2

Sequence conflict

308

1

G → S in CAA24598. Ref.2

Secondary structure

1

.

375

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P60010-1 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: 87AC19B0B0BC9E71
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FASTA

375

41,690

        10         20         30         40         50         60 
MDSEVAALVI DNGSGMCKAG FAGDDAPRAV FPSIVGRPRH QGIMVGMGQK DSYVGDEAQS 

        70         80         90        100        110        120 
KRGILTLRYP IEHGIVTNWD DMEKIWHHTF YNELRVAPEE HPVLLTEAPM NPKSNREKMT 

       130        140        150        160        170        180 
QIMFETFNVP AFYVSIQAVL SLYSSGRTTG IVLDSGDGVT HVVPIYAGFS LPHAILRIDL 

       190        200        210        220        230        240 
AGRDLTDYLM KILSERGYSF STTAEREIVR DIKEKLCYVA LDFEQEMQTA AQSSSIEKSY 

       250        260        270        280        290        300 
ELPDGQVITI GNERFRAPEA LFHPSVLGLE SAGIDQTTYN SIMKCDVDVR KELYGNIVMS 

       310        320        330        340        350        360 
GGTTMFPGIA ERMQKEITAL APSSMKVKII APPERKYSVW IGGSILASLT TFQQMWISKQ 

       370 
EYDESGPSIV HHKCF

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure of a split yeast gene: complete nucleotide sequence of the actin gene in Saccharomyces cerevisiae." Gallwitz D., Sures I. Proc. Natl. Acad. Sci. U.S.A. 77:2546-2550(1980) [PubMed: 6994099] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Isolation and sequence of the gene for actin in Saccharomyces cerevisiae." Ng R., Abelson J. Proc. Natl. Acad. Sci. U.S.A. 77:3912-3916(1980) [PubMed: 7001447] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Lariat structures are in vivo intermediates in yeast pre-mRNA splicing." Domdey H., Apostol B., Lin R.J., Newman A., Brody E., Abelson J. Cell 39:611-621(1984) [PubMed: 6096014] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 4-58.
[4]	"Molecular cloning of the actin gene from yeast Saccharomyces cerevisiae." Gallwitz D., Seidel R. Nucleic Acids Res. 8:1043-1059(1980) [PubMed: 7003553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 5-57.
[5]	"The nucleotide sequences of the actin genes from Saccharomyces carlsbergensis and Saccharomyces cerevisiae are identical except for their introns." Nellen W., Donath C., Moos M., Gallwitz D. J. Mol. Appl. Genet. 1:239-244(1981) [PubMed: 6286825] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Carlsbergensis.
[6]	"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae." Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T. Nat. Genet. 10:261-268(1995) [PubMed: 7670463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[7]	"Partial sequence analysis of the actin gene and its potential for studying the phylogeny of Candida species and their teleomorphs." Daniel H.-M., Sorrell T.C., Meyer W. Int. J. Syst. Evol. Microbiol. 51:1593-1606(2001) [PubMed: 11491363] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 33-358. Strain: ATCC 18824 / CBS 1171 / DSM 70449 / IFO 10217 / NRRL Y-12632 and CBS 1907.
[8]	"Unusual metabolism of the yeast actin amino terminus." Cook R.K., Sheff D.R., Rubenstein P.A. J. Biol. Chem. 266:16825-16833(1991) [PubMed: 1885608] [Abstract] Cited for: ACETYLATION AT MET-1, PROTEIN SEQUENCE OF N-TERMINUS.
[9]	"Protein expression during exponential growth in 0.7 M NaCl medium of Saccharomyces cerevisiae." Norbeck J., Blomberg A. FEMS Microbiol. Lett. 137:1-8(1996) [PubMed: 8935650] [Abstract] Cited for: PROTEIN SEQUENCE OF 19-25. Strain: ATCC 38531 / Y41.
[10]	"Site-directed mutagenesis of the yeast actin gene: a test for actin function in vivo." Johannes F.-Z., Gallwitz D. EMBO J. 10:3951-3958(1991) [PubMed: 1935913] [Abstract] Cited for: MUTAGENESIS.
[11]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-199; SER-201; SER-323 AND SER-324, MASS SPECTROMETRY.
[12]	"The structure of nonvertebrate actin: implications for the ATP hydrolytic mechanism." Vorobiev S., Strokopytov B., Drubin D.G., Frieden C., Ono S., Condeelis J., Rubenstein P.A., Almo S.C. Proc. Natl. Acad. Sci. U.S.A. 100:5760-5765(2003) [PubMed: 12732734] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).
+	Additional computationally mapped references.

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Cross-references

Sequence databases

V01288 Genomic DNA. Translation: CAA24597.1.
V01289 Genomic DNA. Translation: CAA24598.1. Sequence problems.
V01290 Genomic DNA. Translation: CAA24599.1.
L00026 Genomic DNA. Translation: AAA34391.1.
D50617 Genomic DNA. Translation: BAA21512.1.
AJ389075 Genomic DNA. Translation: CAC00716.1.
AJ389076 Genomic DNA. Translation: CAC00717.1.

PIR

ATBY. A03005.
JS0702.

RefSeq

NP_116614.1.