Reviewed, UniProtKB/Swiss-Prot P61697 (SYA_BACC1)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	BCE_4471

Organism

Bacillus cereus (strain ATCC 10987) [Complete proteome] [HAMAP]

Taxonomic identifier

222523 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	880 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala).
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 880	880	Alanyl-tRNA synthetase

Sequences

	Sequence		Length	Mass (Da)
	P61697-1 [UniParc]. Last modified June 7, 2004. Version 1. Checksum: 8DF9F2B9531DB18F	FASTA	880	97,414
	10 20 30 40 50 60 MKQLTGAQIR QMFLDFFQEK GHAVEPSASL VPHEDPSLLW INSGVATLKK YFDGRVIPQN 70 80 90 100 110 120 PRITNAQKSI RTNDIENVGK TARHHTFFEM LGNFSIGDYF KEEAITWAWE FLTSDKWIGF 130 140 150 160 170 180 DKELLSVTIH PEDEEAFTIW NEKMGVPKER IIRLEENFWD IGEGPSGPNT EIFYDRGEAY 190 200 210 220 230 240 GNDFSDPELY PGGENERYLE VWNLVFSQFN HNPDGSYTPL PKKNIDTGMG LERMTSIVQD 250 260 270 280 290 300 VPTNFDTDLF MPMIGATETI SGEKYRNGDL EKDMAFKVIA DHIRTVTFAV GDGALPSNEG 310 320 330 340 350 360 RGYVLRRLLR RAVRYSKKLN INRPFMFELV PVVGEVMKDF YPEVLEKKDF IAKVVKNEEE 370 380 390 400 410 420 RFHETLHDGE AILAEVIAKA KEEKTTVISG VDAFRLYDTY GFPIELTEEY AEEAGMTVDH 430 440 450 460 470 480 EGFENEMEKQ RERARAARQD VDSMQVQGGV LGEVKVASEF VGYGTVATES NVVALVKNGE 490 500 510 520 530 540 YADSLQAGEE GQLMLDVTPF YAESGGQIAD RGYLLADGVK VLVKDVQKAP NGQNLHKVVV 550 560 570 580 590 600 EEGTLTKDAA VKAIIDTKNR SSVVKNHTAT HLLHQALKDV LGTHVNQAGS LVTSERLRFD 610 620 630 640 650 660 FSHFGQVQAD ELEKIERIVN EKIWESIDVE ISQKAIEEAK EMGAMALFGE KYGDVVRVVQ 670 680 690 700 710 720 VGDYSLELCG GCHVDNTASI GIFKIVAESG IGAGTRRIEA VTGKSAYELM NDQVGLLKEA 730 740 750 760 770 780 AGKMKTNPKD ILTRVDGLFA EVKQLQKENE SLAAKLSNIE AGNLTDSVMT VDGVNVLAAK 790 800 810 820 830 840 VNVADMNNLR TMMDDLKNKL QSAVVVLASV NDDKVNILAG VTKDLISQGY HAGKLVKEVA 850 860 870 880 SRCGGGGGGR PDMAQAGGKN PAQVEEALAF VQEYVKSVSK

References

[1]

"The genome sequence of Bacillus cereus ATCC 10987 reveals metabolic adaptations and a large plasmid related to Bacillus anthracis pXO1." Rasko D.A., Ravel J., Oekstad O.A., Helgason E., Cer R.Z., Jiang L., Shores K.A., Fouts D.E., Tourasse N.J., Angiuoli S.V., Kolonay J.F., Nelson W.C., Kolstoe A.-B., Fraser C.M., Read T.D. Nucleic Acids Res. 32:977-988(2004) [PubMed: 14960714] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017194 Genomic DNA. Translation: AAS43372.1.
RefSeq	NP_980764.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2752228.
GenomeReviews	Gene locus BCE_4471 in contig AE017194_GR.
KEGG	bca:BCE_4471.
NMPDR	fig|222523.1.peg.4436.
TIGR	BCE_4471.
Phylogenomic databases
HOGENOM	P61697.
Family and domain databases
HAMAP	MF_00036. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR003156. Pesterase_DHHA1. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProDom	P61697. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

SYA_BACC1

Accession

Primary (citable) accession number: P61697

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2004
Last sequence update:	June 7, 2004
Last modified:	July 22, 2008
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)