Reviewed, UniProtKB/Swiss-Prot P61699 (SYA_CORDI)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	DIP1350

Organism

Corynebacterium diphtheriae [Complete proteome] [HAMAP]

Taxonomic identifier

1717 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	888 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala).
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 888	888	Alanyl-tRNA synthetase

Sequences

	Sequence		Length	Mass (Da)
	P61699-1 [UniParc]. Last modified June 7, 2004. Version 1. Checksum: 4AB1F6B6FCB8FD0D	FASTA	888	96,354
	10 20 30 40 50 60 MQTHEIRERF TNHFVKAGHE AVPSASLILD DPNLLFVNAG MVPFKPYFLG QQNPPFPNGT 70 80 90 100 110 120 ATSIQKCVRT LDIEEVGITT RHNTFFQMAG NFSFGQYFKE GAITHAWQLL TGTVAEGGLG 130 140 150 160 170 180 LDPERLWVTV YLDDDEAAEI WNKKIGVPSE RIQRLGMADN YWSMGIPGPC GPCSEIYYDR 190 200 210 220 230 240 GPEYGAEGGP IADDNRYMEI WNLVFMENER GEGIGKDNFE IVGPLPKKNI DTGMGIERVA 250 260 270 280 290 300 CILQGVDNVY ETDLLRPVIN VAEELTGATY GTNDDDNIRF RVIADHSRTG MMLILDGVTP 310 320 330 340 350 360 GNEGRGYILR RLLRRIIRSA HLLGAKGKTL EKFMNTIMDT MTPSYPEIAD NRERILRVAI 370 380 390 400 410 420 TEEKAFLKTL ASGTELFEDK ADQVKNSGNK VLDGAAAFKL HDTYGFPIDL TLEMAAEAGL 430 440 450 460 470 480 NVDIEGFNSL MAEQRARAKA DNRAKKHGHA DLSIYREWVD EHPTVFTGYD ELTTDSKVLG 490 500 510 520 530 540 LLSAGNKVNE IKAGEEVEVI LEASPLYAEA GGQLGDRGQI LMGDTVLKVN DVQKIGKKLW 550 560 570 580 590 600 VHKATVAQGG LSVGDVVTAQ VDSDWRHAAR QAHTGTHLIH AALRQVLGPT AVQAGSMNKP 610 620 630 640 650 660 GYLRFDFNYT ESLTPRQLEE IQNITNQAVD SDWDVNTIET SLEEAKAMGA LALFGENYGS 670 680 690 700 710 720 EVRVIEVGGP FSIELCGGTH VTHSSQIGPV AILGESSVGS GVRRIEAYTG LDSFKYLAKE 730 740 750 760 770 780 QALVENIASS MKVKPEELPD RIAALTQKLK DAEKAIADMR SAQLMAQATG LVAGAIEVNG 790 800 810 820 830 840 VKLVAQRVSD VENMGDLRSL AADIKQRLDD VPAVVALVGE DASGKAPFVV GATKAAVSAG 850 860 870 880 YKANAYAKIL GQYIEGNGGG KPDMAQGSAR NSSGFDQGIA AVKAEMSA

References

[1]

"The complete genome sequence and analysis of Corynebacterium diphtheriae NCTC13129." Cerdeno-Tarraga A.-M., Efstratiou A., Dover L.G., Holden M.T.G., Pallen M.J., Bentley S.D., Besra G.S., Churcher C.M., James K.D., De Zoysa A., Chillingworth T., Cronin A., Dowd L., Feltwell T., Hamlin N., Holroyd S., Jagels K., Moule S. , Quail M.A., Rabbinowitsch E., Rutherford K.M., Thomson N.R., Unwin L., Whitehead S., Barrell B.G., Parkhill J. Nucleic Acids Res. 31:6516-6523(2003) [PubMed: 14602910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700971 / NCTC 13129 / Biotype gravis.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX248357 Genomic DNA. Translation: CAE49878.1.
RefSeq	NP_939703.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2648793.
GenomeReviews	Gene locus DIP1350 in contig BX248353_GR.
KEGG	cdi:DIP1350.
NMPDR	fig|257309.1.peg.1294.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P61699.
Enzyme and pathway databases
BioCyc	CDIP257309:DIP1350-MON.
Family and domain databases
HAMAP	MF_00036. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR003156. Pesterase_DHHA1. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProDom	P61699. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

SYA_CORDI

Accession

Primary (citable) accession number: P61699

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2004
Last sequence update:	June 7, 2004
Last modified:	July 22, 2008
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)