Reviewed, UniProtKB/Swiss-Prot P62739 (ACTA_BOVIN)

Names and origin

Protein names

Recommended name:
    Actin, aortic smooth muscle
Alternative name(s):
    Alpha-actin-2

Gene names

Name:	ACTA2
Synonyms:	ACTSA, ACTVS

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	377 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Subunit structure	Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others.
Subcellular location	Cytoplasm › cytoskeleton.
Miscellaneous	In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.
Sequence similarities	Belongs to the actin family.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton
Ligand	ATP-binding Nucleotide-binding
Molecular function	Muscle protein Structural protein
PTM	Acetylation Methylation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	muscle thin filament assembly Inferred from sequence or structural similarity. Source: AgBase
Cellular component	actin filament Inferred from sequence or structural similarity. Source: AgBase cytosol Inferred from sequence or structural similarity. Source: AgBase soluble fraction Inferred from sequence or structural similarity. Source: AgBase stress fiber Inferred from sequence or structural similarity. Source: AgBase striated muscle thin filament Inferred from sequence or structural similarity. Source: AgBase
Molecular function	protein binding Inferred from sequence or structural similarity. Source: AgBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Propeptide	1 – 2	2	Removed in mature form
	Chain	3 – 377	375	Actin, aortic smooth muscle
Amino acid modifications
	Modified residue	3	1	N-acetylglutamate
	Modified residue	75	1	Tele-methylhistidine

Sequences

	Sequence		Length	Mass (Da)
	P62739-1 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: 2D0543262DB35CA5	FASTA	377	42,009
	10 20 30 40 50 60 MCEEEDSTAL VCDNGSGLCK AGFAGDDAPR AVFPSIVGRP RHQGVMVGMG QKDSYVGDEA 70 80 90 100 110 120 QSKRGILTLK YPIEHGIITN WDDMEKIWHH SFYNELRVAP EEHPTLLTEA PLNPKANREK 130 140 150 160 170 180 MTQIMFETFN VPAMYVAIQA VLSLYASGRT TGIVLDSGDG VTHNVPIYEG YALPHAIMRL 190 200 210 220 230 240 DLAGRDLTDY LMKILTERGY SFVTTAEREI VRDIKEKLCY VALDFENEMA TAASSSSLEK 250 260 270 280 290 300 SYELPDGQVI TIGNERFRCP ETLFQPSFIG MESAGIHETT YNSIMKCDID IRKDLYANNV 310 320 330 340 350 360 LSGGTTMYPG IADRMQKEIT ALAPSTMKIK IIAPPERKYS VWIGGSILAS LSTFQQMWIS 370 KQEYDEAGPS IVHRKCF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Ileum.
[2]	"The complete amino acid sequence of actins from bovine aorta, bovine heart, bovine fast skeletal muscle, and rabbit slow skeletal muscle. A protein-chemical analysis of muscle actin differentiation." Vandekerckhove J., Weber K. Differentiation 14:123-133(1979) [PubMed: 499690] [Abstract] Cited for: PROTEIN SEQUENCE OF 3-377, METHYLATION AT HIS-75.
[3]	"Characterization of gene expression profiles in early bovine pregnancy using a custom cDNA microarray." Ishiwata H., Katsuma S., Kizaki K., Patel O.V., Nakano H., Takahashi T., Imai K., Hirasawa A., Shiojima S., Ikawa H., Suzuki Y., Tsujimoto G., Izaike Y., Todoroki J., Hashizume K. Mol. Reprod. Dev. 65:9-18(2003) [PubMed: 12658628] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 260-377.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC102699 mRNA. Translation: AAI02700.1. AB098797 mRNA. Translation: BAC56333.1.
PIR	ATBOSM. A02997.
RefSeq	NP_001029674.1.
UniGene	Bt.37349
3D structure databases
SMR	P62739. Positions 6-373.
ModBase	Search...
Protein-protein interaction databases
IntAct	P62739.
Genome annotation databases
Ensembl	ENSBTAG00000014614. Bos taurus. [Contig view]
GeneID	515610.
KEGG	bta:515610.
Phylogenomic databases
HOVERGEN	P62739.
Family and domain databases
InterPro	IPR004001. Actin_CS. IPR004000. Actin_like. [Graphical view]
PANTHER	PTHR11937. Actin_like. 1 hit.
Pfam	PF00022. Actin. 1 hit. [Graphical view]
PRINTS	PR00190. ACTIN.
SMART	SM00268. ACTIN. 1 hit. [Graphical view]
PROSITE	PS00406. ACTINS_1. 1 hit. PS00432. ACTINS_2. 1 hit. PS01132. ACTINS_ACT_LIKE. 1 hit. [Graphical view]
ProDom	P62739. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

ACTA_BOVIN

Accession

Primary (citable) accession number: P62739
Secondary accession number(s): P03996 , P04108, Q3ZCA0, Q862W5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 23, 1986
Last sequence update:	August 16, 2004
Last modified:	July 22, 2008
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)