Reviewed, UniProtKB/Swiss-Prot P67008 (SYA_BRUME)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	BMEI0789

Organism

Brucella melitensis [Complete proteome] [HAMAP]

Taxonomic identifier

29459 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	885 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala).
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Domain	Zinc-finger
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 885	885	Alanyl-tRNA synthetase
Regions
	Zinc finger	169 – 182	14	C2H2-type

Sequences

	Sequence		Length	Mass (Da)
	P67008-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 299DCF5FAEC1EEC4	FASTA	885	95,586
	10 20 30 40 50 60 MAGVNEIRST FLDYFRKNGH EVVPSSPLVP RNDPTLMFTN AGMVQFKNVF TGLEHRSYNR 70 80 90 100 110 120 ATTSQKCVRA GGKHNDLDNV GYTARHHTFF EMLGNFSFGD YFKEDAISFA WNLITREFGL 130 140 150 160 170 180 PKDKLLVTVY HTDDDAANFW KKIAGLSDDR IIRIPTSDNF WAMGDTGPCG PCSEIFYDHG 190 200 210 220 230 240 DHIWGGPPGS PEEDGDRFIE IWNLVFMQFE QQTPELRIDL PRPSIDTGMG LERIAAVLQG 250 260 270 280 290 300 VHDNYDIDLF KALIRASEEA TGVKAEGDFR ASHRVIADHL RASSFLIADG VLPSNEGRGY 310 320 330 340 350 360 VLRRIMRRAM RHAQLLGAKE PLMWRLLPAL IREMGQAYPE LIRAESLISE TLKLEETRFR 370 380 390 400 410 420 KTLERGLGLL SDASENLAEG DRLDGETAFK LYDTYGFPLD LTQDALRQRG IAVDTEGFNV 430 440 450 460 470 480 AMERQKAEAR ANWTGSGEAA TETIWFGIKD KVGATEFLGY ETESAEGVIA SLVRDGVEVP 490 500 510 520 530 540 SVREGETISV VVNQTPFYGE SGGQQGDTGT ISGEGFVIAV KDTQKKGEGV FVHIGEVTEG 550 560 570 580 590 600 TAKAGDVVEL KVDSARRTRI RSNHSATHLL HEALRETLGT HVAQKGSLVA PDRLRFDFSH 610 620 630 640 650 660 PKPISAEELE AVENLANEII LQNAPVTTRL MAVDDAIAEG AMALFGEKYG DEVRVVSMGT 670 680 690 700 710 720 AKHGSKAGKA YSVELCGGTH VRQTGDIGLV RIISEGGVAA GVRRLEALTG EAARLYLEEQ 730 740 750 760 770 780 DERVKAIASA LKTTSADVLD RVNALIDERK KLERELADAR KKLALGGGSS DGGSAVEAVN 790 800 810 820 830 840 GVNFLGKIVT GVSPRDLKPL ADEGKKQVGS GVVLFIGVGE DGKASAVAAV TEDMVGRFSA 850 860 870 880 VDLVRAASAA LGGAGGGGRP DMAQAGGPDG AKAADAIAAV KALIA

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094 / Biotype 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE009520 Genomic DNA. Translation: AAL51970.1.
PIR	AG3350.
RefSeq	NP_539706.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1196500.
GenomeReviews	Gene locus BMEI0789 in contig AE008917_GR.
KEGG	bme:BMEI0789.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P67008.
Enzyme and pathway databases
BioCyc	BMEL224914:BMEI0789-MON.
Family and domain databases
HAMAP	MF_00036. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR003156. Pesterase_DHHA1. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProDom	P67008. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

SYA_BRUME

Accession

Primary (citable) accession number: P67008
Secondary accession number(s): Q8YHK8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	July 22, 2008
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)