Reviewed, UniProtKB/Swiss-Prot P68301 (MT2_BOVIN)

Names and origin

Protein names

Recommended name:
    Metallothionein-2
      Short name=MT-2
Alternative name(s):
    Metallothionein-II
      Short name=MT-II

Gene names

Name:	MT2
Synonyms:	MT2A

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	61 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids.
Domain	Class I metallothioneins contain 2 metal-binding domains: four divalent ions are chelated within cluster A of the alpha domain and are coordinated via cysteinyl thiolate bridges to 11 cysteine ligands. Cluster B, the corresponding region within the beta domain, can ligate three divalent ions to 9 cysteines.
Sequence similarities	Belongs to the metallothionein superfamily. Type 1 family.

Ontologies

Keywords
Ligand	Metal-binding Metal-thiolate cluster
PTM	Acetylation
Technical term	Direct protein sequencing
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 61	61	Metallothionein-2
Regions
	Region	1 – 29	29	Beta
	Region	30 – 61	32	Alpha
Sites
	Metal binding	5	1	Cluster B
	Metal binding	7	1	Cluster B
	Metal binding	13	1	Cluster B
	Metal binding	15	1	Cluster B
	Metal binding	19	1	Cluster B
	Metal binding	21	1	Cluster B
	Metal binding	24	1	Cluster B
	Metal binding	26	1	Cluster B
	Metal binding	29	1	Cluster B
	Metal binding	33	1	Cluster A
	Metal binding	34	1	Cluster A
	Metal binding	36	1	Cluster A
	Metal binding	37	1	Cluster A
	Metal binding	41	1	Cluster A
	Metal binding	44	1	Cluster A
	Metal binding	48	1	Cluster A
	Metal binding	50	1	Cluster A
	Metal binding	57	1	Cluster A
	Metal binding	59	1	Cluster A
	Metal binding	60	1	Cluster A
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine
	Modified residue	51	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P68301-1 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 7B0A626F8121F1D3	FASTA	61	6,028
	10 20 30 40 50 60 MDPNCSCTAG ESCTCAGSCK CKDCKCASCK KSCCSCCPVG CAKCAQGCVC KGASDKCSCC A

References

[1]	"(Cu,Zn)-metallothioneins from fetal bovine liver. Chemical and spectroscopic properties." Muenger K., Germann U.A., Beltramini M., Niedermann D., Baitella-Eberle G., Kaegi J.H.R., Lerch K. J. Biol. Chem. 260:10032-10038(1985) [PubMed: 4019500] [Abstract] Cited for: PROTEIN SEQUENCE, ACETYLATION AT MET-1. Tissue: Liver.
[2]	"Bovine metallothioneins in Italian population." Salvini M., Conte G., Taddei S., Durante M. Submitted (JUN-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ489256 mRNA. Translation: CAD33256.1.
PIR	B23889.
RefSeq	XP_586929.3.
UniGene	Bt.2529
3D structure databases
HSSP	HSSP built from PDB template 1MHU based on UniProtKB P02795.
SMR	P68301. Positions 1-61.
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAG00000023659. Bos taurus. [Contig view]
GeneID	404070.
KEGG	bta:404070.
Phylogenomic databases
HOVERGEN	P68301.
Family and domain databases
InterPro	IPR002400. GF_cysknot. IPR003019. Metallothionein_sfam_euk. IPR000006. Metallothionein_vert. [Graphical view]
Gene3D	G3DSA:4.10.10.10. Metallothionein_vert. 1 hit.
PANTHER	PTHR23299. Metallothionein_vert. 1 hit.
Pfam	PF00131. Metallothio. 1 hit. [Graphical view]
PRINTS	PR00438. GFCYSKNOT. PR00860. MTVERTEBRATE.
PROSITE	PS00203. METALLOTHIONEIN_VRT. 1 hit. [Graphical view]
ProDom	P68301. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

MT2_BOVIN

Accession

Primary (citable) accession number: P68301
Secondary accession number(s): P09579, Q546G1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2004
Last sequence update:	October 25, 2004
Last modified:	July 22, 2008
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)