Reviewed, UniProtKB/Swiss-Prot P68497 (MT_CHICK)

Names and origin

Protein names	Recommended name:     Metallothionein       Short name(s)=MT
Organism	Gallus gallus (Chicken)
Taxonomic identifier	9031 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	63 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Metallothioneins have a high content of cysteine residues that bind various heavy metals.
Domain	Class I metallothioneins contain 2 metal-binding domains: four divalent ions are chelated within cluster A of the alpha domain and are coordinated via cysteinyl thiolate bridges to 11 cysteine ligands. Cluster B, the corresponding region within the beta domain, can ligate three divalent ions to 9 cysteines.
Sequence similarities	Belongs to the metallothionein superfamily. Type 1 family.

Ontologies

Keywords
Ligand	Metal-binding Metal-thiolate cluster Zinc
Technical term	Direct protein sequencing
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 63	63	Metallothionein
Regions
	Region	1 – 30	30	Beta
	Region	31 – 63	33	Alpha
Sites
	Metal binding	6	1	Cluster B
	Metal binding	8	1	Cluster B
	Metal binding	14	1	Cluster B
	Metal binding	16	1	Cluster B
	Metal binding	20	1	Cluster B
	Metal binding	22	1	Cluster B
	Metal binding	25	1	Cluster B
	Metal binding	27	1	Cluster B
	Metal binding	30	1	Cluster B
	Metal binding	34	1	Cluster A
	Metal binding	35	1	Cluster A
	Metal binding	37	1	Cluster A
	Metal binding	38	1	Cluster A
	Metal binding	42	1	Cluster A
	Metal binding	45	1	Cluster A
	Metal binding	49	1	Cluster A
	Metal binding	51	1	Cluster A
	Metal binding	59	1	Cluster A
	Metal binding	61	1	Cluster A
	Metal binding	62	1	Cluster A

Sequences

	Sequence		Length	Mass (Da)
	P68497-1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 0062422501B9CC84	FASTA	63	6,462
	10 20 30 40 50 60 MDPQDCTCAA GDSCSCAGSC KCKNCRCRSC RKSCCSCCPA GCNNCAKGCV CKEPASSKCS CCH

References

[1]	"Amino acid sequence and comparative antigenicity of chicken metallothionein." McCormick C.C., Fullmer C.S., Garvey J.S. Proc. Natl. Acad. Sci. U.S.A. 85:309-313(1988) [PubMed: 2448773] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Liver.
[2]	"Molecular cloning of chicken metallothionein. Deduction of the complete amino acid sequence and analysis of expression using cloned cDNA." Wei D., Andrews G.K. Nucleic Acids Res. 16:537-553(1988) [PubMed: 3340548] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: White leghorn.
[3]	"Structure and expression of chicken metallothionein." Fernando L.P., Wei D., Andrews G.K. J. Nutr. 119:309-318(1989) [PubMed: 2645390] [Abstract] Cited for: PROTEIN SEQUENCE.
[4]	"Cloning and expression of an avian metallothionein-encoding gene." Fernando L.P., Andrews G.K. Gene 81:177-183(1989) [PubMed: 2806910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X06749 mRNA. Translation: CAA29924.1. X13545 Genomic DNA. No translation available.
PIR	A34958.
RefSeq	NP_990606.1.
UniGene	Gga.4210
3D structure databases
HSSP	HSSP built from PDB template 2MHU based on UniProtKB P02795.
ModBase	Search...
Genome annotation databases
GeneID	396212.
KEGG	gga:396212.
Phylogenomic databases
HOVERGEN	P68497.
Family and domain databases
InterPro	IPR015874. 4-disulphide_core. IPR002400. GF_cysknot. IPR003019. Metallothionein_sfam_euk. IPR000006. Metallothionein_vert. IPR001008. Metallthion_2. [Graphical view]
Gene3D	G3DSA:4.10.10.10. Metallothionein_vert. 1 hit.
PANTHER	PTHR23299. Metallothionein_vert. 1 hit.
Pfam	PF00131. Metallothio. 1 hit. [Graphical view]
PRINTS	PR00003. 4DISULPHCORE. PR00438. GFCYSKNOT. PR00875. MTMOLLUSC. PR00860. MTVERTEBRATE.
PROSITE	PS00203. METALLOTHIONEIN_VRT. 1 hit. [Graphical view]
ProDom	P68497. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

MT_CHICK

Accession

Primary (citable) accession number: P68497
Secondary accession number(s): P09576

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 23, 2004
Last sequence update:	November 23, 2004
Last modified:	July 22, 2008
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)