Reviewed, UniProtKB/Swiss-Prot P70268 (PKN1_MOUSE)

Names and origin

Protein names

Recommended name:
    Serine/threonine-protein kinase N1
    EC=2.7.11.13
Alternative name(s):
    Protein kinase C-like 1
    Protein-kinase C-related kinase 1
    Protein kinase C-like PKN
    Serine-threonine protein kinase N

Gene names

Name:	Pkn1
Synonyms:	Pkn, Prk1, Prkcl1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	946 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Can phosphorylate ribosomal protein S6. Mediates GTPase Rho dependent intracellular signaling By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation	Activated by lipids, particularly cardiolipin and to a lesser extent by other acidic phospholipids. Two specific sites, Thr-778 (activation loop of the kinase domain) and Ser-920 (turn motif), need to be phosphorylated for its full activation By similarity.
Subunit structure	Interacts with RhoA and Rac1 By similarity. Interacts with ZA20D3.
Subcellular location	CytoplasmBy similarity.
Domain	The C1 domain does not bind the diacylglycerol (DAG).
Post-translational modification	Autophosphorylated; preferably on serine By similarity. Activated by limited proteolysis with trypsin By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 C2 domain. Contains 1 protein kinase domain. Contains 3 REM (Hr1) repeats.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	hyperosmotic response Inferred from direct assay. Source: MGI
Cellular component	cytoplasmic membrane-bounded vesicle Inferred from direct assay. Source: MGI
Molecular function	protein kinase activity Inferred from mutant phenotype. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 946	946	Serine/threonine-protein kinase N1
Regions
	Repeat	34 – 110	77	REM 1
	Repeat	123 – 212	90	REM 2
	Repeat	213 – 294	82	REM 3
	Domain	328 – 464	137	C2
	Domain	619 – 878	260	Protein kinase
	Domain	879 – 946	68	AGC-kinase C-terminal
	Nucleotide binding	625 – 633	9	ATP By similarity
Sites
	Active site	744	1	Proton acceptor By similarity
	Binding site	648	1	ATP By similarity
Amino acid modifications
	Modified residue	379	1	Phosphoserine By similarity
	Modified residue	565	1	Phosphoserine By similarity
	Modified residue	778	1	Phosphothreonine Probable
	Modified residue	782	1	Phosphothreonine By similarity
	Modified residue	920	1	Phosphoserine

Sequences

	Sequence		Length	Mass (Da)
	P70268-1 [UniParc]. Last modified November 23, 2004. Version 3. Checksum: FCB4E61AD27C1271	FASTA	946	104,411
	10 20 30 40 50 60 MAGDAVQSEP RSWSLLEQLG LAGADLAAPG VQQQLELERE RLKREIRKEL KLKEGAENLR 70 80 90 100 110 120 RATTDLGRSL APVELLLRGS ARRLDLLHQQ LQELHAHVVL PDPAAGSDAT QSLAEGSPIC 130 140 150 160 170 180 SSTNLSRVAG LEKQLAIELK VKQGAENMIQ TYSNGSSKDR KLLLTAQQML QDSKTKIDII 190 200 210 220 230 240 RMQLRRALQA LQAGELESQA APDEAQGDPE LGAVELRIEE LRHHFRVEHA VAEGAKNVLR 250 260 270 280 290 300 LLSGAKAPDR KAVSEAQEKL TESNQKLGLL RESLERRLGE LPADHPKGRL LREELTAASS 310 320 330 340 350 360 SAFSAILPGP FPATHYSTLS KPAPLTGTLE VRVVGCKNLP ETIPWSPPPS VGASGTPESR 370 380 390 400 410 420 TPFLSRPARG LYSRSGSLSG RSSLRGEAEN ATEVSTVLKL DNTVVGQTAW KPCGPNAWDQ 430 440 450 460 470 480 SFTLELERAR ELELAVFWRD QRGLCALKFL KLEDFLDNER HEVQLDMEPQ GCLVAEVTFR 490 500 510 520 530 540 NPIIERIPRL QRQKKIFSKQ QGKAFQRARQ MNIDVATWVR LLRRLIPSAV ATGTFSPNAS 550 560 570 580 590 600 PGAEIRHTGD ISMEKLNLGA DSDSSSQKSP PGLPSTSCSL SSPTHESTTS PELPSETQET 610 620 630 640 650 660 PGPGLCSPLR KSPLTLEDFK FLAVLGRGHF GKVLLSEFRS SGELFAIKAL KKGDIVARDE 670 680 690 700 710 720 VESLMCEKRI LAAVTRAGHP FLVNLFGCFQ TPEHVCFVME YSAGGDLMLH IHSDVFSEPR 730 740 750 760 770 780 AVFYSACVVL GLQFLHEHKI VYRDLKLDNL LLDTEGYVKI ADFGLCKEGM GYGDRTSTFC 790 800 810 820 830 840 GTPEFLAPEV LTDTSYTRAV DWWGLGVLLY EMLVGESPFP GDDEEEVFDS IVNDEVRYPR 850 860 870 880 890 900 FLSAEAIGIM RRLLRRNPER RLGSTERDAE DVKKQPFFRS LGWDVLLARR LPPPFVPTLS 910 920 930 940 GRTDVSNFDE EFTGEAPTLS PPRDARPLTA AEQAAFRDFD FVAGGY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Olfactory epithelium.
[2]	"The mouse genes for the EP1 prostanoid receptor and the PKN protein kinase overlap." Batshake B., Sundelin S. Biochem. Biophys. Res. Commun. 227:70-76(1996) [PubMed: 8858105] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 664-946. Strain: 129.
[3]	"Cloning and characterization of AWP1, a novel protein that associates with serine/threonine kinase PRK1 in vivo." Duan W., Sun B., Li T.W., Tan B.J., Lee M.K., Teo T.S. Gene 256:113-121(2000) [PubMed: 11054541] [Abstract] Cited for: INTERACTION WITH ZA20D3. Tissue: Liver, Mammary gland and Uterus.
[4]	"Phosphoproteomic analysis of the developing mouse brain." Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P. Mol. Cell. Proteomics 3:1093-1101(2004) [PubMed: 15345747] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-920, MASS SPECTROMETRY. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC052923 mRNA. Translation: AAH52923.1. Y07611 Genomic DNA. Translation: CAA68883.1.
PIR	PC4220.
RefSeq	NP_796236.2.
UniGene	Mm.213000
3D structure databases
HSSP	HSSP built from PDB template 1GZK based on UniProtKB P31751.
SMR	P70268. Positions 13-98, 122-202.
ModBase	Search...
PTM databases
PhosphoSite	P70268.
Genome annotation databases
Ensembl	ENSMUSG00000057672. Mus musculus. [Contig view]
GeneID	320795.
KEGG	mmu:320795.
Organism-specific databases
MGI	MGI:108022. Pkn1.
Phylogenomic databases
HOVERGEN	P70268.
Gene expression databases
CleanEx	MM_PKN1.
GermOnline	ENSMUSG00000057672. Mus musculus.
Family and domain databases
InterPro	IPR000008. C2_Ca-dep. IPR000861. HR1-like_rho-bd. IPR011072. HR1_rho-bd. IPR000961. Pkinase_C. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_bd_CS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Gene3D	G3DSA:1.10.287.160. HR1_rho-bd. 3 hits.
Pfam	PF02185. HR1. 3 hits. PF00069. Pkinase. 1 hit. PF00433. Pkinase_C. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00239. C2. 1 hit. SM00742. Hr1. 3 hits. SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS51285. AGC_KINASE_CTER. 1 hit. PS50004. C2. False negative. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
BLOCKS	Search...
Other Resources
SOURCE	Search...
ProtoNet	Search...

Entry information

Entry name

PKN1_MOUSE

Accession

Primary (citable) accession number: P70268
Secondary accession number(s): Q7TST2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 23, 2004
Last modified:	July 22, 2008
This is version 84 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)