Reviewed, UniProtKB/Swiss-Prot P70269 (CATE_MOUSE)

Names and origin

Protein names

Recommended name:
    Cathepsin E
    EC=3.4.23.34

Gene names

Name:

Ctse

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	397 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May have a role in immune function. Probably involved in the processing of antigenic peptides during MHC class II-mediated antigen presentation. May play a role in activation-induced lymphocyte depletion in the thymus, and in neuronal degeneration and glial cell activation in the brain.
Catalytic activity	Similar to cathepsin D, but slightly broader specificity.
Subunit structure	Homodimer; disulfide-linked By similarity.
Subcellular location	Endosome. Note= The proenzyme is localized to the endoplasmic reticulum and Golgi apparatus, while the mature enzyme is localized to the endosome.
Tissue specificity	Expressed abundantly in the stomach, Clara cells and alveolar macrophages of the lung, brain microglia, spleen and activated B-lymphocytes. Not expressed in resting B-lymphocytes.
Sequence similarities	Belongs to the peptidase A1 family.

Ontologies

Keywords
Cellular component	Endosome
Domain	Signal
Molecular function	Aspartyl protease Hydrolase Protease
PTM	Glycoprotein Pyrrolidone carboxylic acid Zymogen
Gene Ontology (GO)
Biological process	antigen processing and presentation of exogenous peptide antigen via MHC class II Ref.6 Inferred from direct assay. Source: UniProtKB
Cellular component	endosome Ref.5 Inferred from direct assay. Source: UniProtKB
Molecular function	cathepsin E activity Ref.1 Inferred from direct assay. Source: UniProtKB protein homodimerization activity Inferred from direct assay. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Signal peptide	1 – 18	18	By similarity
	Propeptide	19 – 59	41	Activation peptide By similarity
	Chain	60 – 397	338	Cathepsin E
Sites
	Active site	97	1	By similarity
	Active site	282	1	By similarity
Amino acid modifications
	Modified residue	19	1	Pyrrolidone carboxylic acid By similarity
	Glycosylation	91	1	N-linked (GlcNAc...) Potential
	Glycosylation	323	1	N-linked (GlcNAc...) Potential
	Disulfide bond	61		Interchain Probable
	Disulfide bond	110 ↔ 115		By similarity
	Disulfide bond	273 ↔ 277		By similarity
Experimental info
	Sequence conflict	297	1	H → Q Ref.2 Ref.3
	Sequence conflict	347	1	D → E in CAA08880. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P70269-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 83993FFE3AB36105	FASTA	397	42,933
	10 20 30 40 50 60 MKPLLVLLLL LLLDLAQAQG ALHRVPLRRH QSLRKKLRAQ GQLSEFWRSH NLDMTRLSES 70 80 90 100 110 120 CNVYSSVNEP LINYLDMEYF GTISIGTPPQ NFTVIFDTGS SNLWVPSVYC TSPACKAHPV 130 140 150 160 170 180 FHPSQSDTYT EVGNHFSIQY GTGSLTGIIG ADQVSVEGLT VDGQQFGESV KEPGQTFVNA 190 200 210 220 230 240 EFDGILGLGY PSLAAGGVTP VFDNMMAQNL VALPMFSVYL SSDPQGGSGS ELTFGGYDPS 250 260 270 280 290 300 HFSGSLNWIP VTKQAYWQIA LDGIQVGDTV MFCSEGCQAI VDTGTSLITG PPDKIKHLQE 310 320 330 340 350 360 AIGATPIDGE YAVDCATLDT MPNVTFLINE VSYTLNPTDY ILPDLVDGMQ FCGSGFQGLD 370 380 390 IPPPAGPLWI LGDVFIRQFY SVFDRGNNQV GLAPAVP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, expression and characterisation of murine procathepsin E." Tatnell P.J., Lees W.E., Kay J. FEBS Lett. 408:62-66(1997) [PubMed: 9180269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY. Strain: BALB/c. Tissue: Spleen.
[2]	Tatnell P.J., Roth W., Duessing J., Kay J., Peters C. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: 129/SvJ.
[3]	"Cathepsin E gene in mouse." Yonezawa S., Masaki S., Hanai A., Ono T., Sonta S., Hirai H., Ichinose M., Miki K., Takahashi K., Kageyama T. Biomed. Res. 19:327-334(1998) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY. Strain: 129/SvJ. Tissue: Gastric mucosa.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Mammary gland.
[5]	"Antigen processing for presentation by class II major histocompatibility complex requires cleavage by cathepsin E." Bennett K., Levine T., Ellis J.S., Peanasky R.J., Samloff I.M., Kay J., Chain B.M. Eur. J. Immunol. 22:1519-1524(1992) [PubMed: 1601038] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[6]	"Involvement of cathepsin E in exogenous antigen processing in primary cultured murine microglia." Nishioku T., Hashimoto K., Yamashita K., Liou S.-Y., Kagamiishi Y., Maegawa H., Katsube N., Peters C., von Figura K., Saftig P., Katunuma N., Yamamoto K., Nakanishi H. J. Biol. Chem. 277:4816-4822(2002) [PubMed: 11719510] [Abstract] Cited for: FUNCTION.
[7]	"Regulation of human and mouse procathepsin E gene expression." Cook M., Caswell R.C., Richards R.J., Kay J., Tatnell P.J. Eur. J. Biochem. 268:2658-2668(2001) [PubMed: 11322887] [Abstract] Cited for: TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X97399 mRNA. Translation: CAA66056.1. Y10928 Genomic DNA. Translation: CAA71859.1. AJ009840 , AJ009841, AJ009842, AJ009843, AJ009844, AJ009845, AJ009846, AJ009847, AJ009848 Genomic DNA. Translation: CAA08880.2. BC005432 mRNA. Translation: AAH05432.1.
UniGene	Mm.230249
3D structure databases
HSSP	HSSP built from PDB template 4CMS based on UniProtKB P00794.
SMR	P70269. Positions 69-396.
ModBase	Search...
Protein family/group databases
MEROPS	A01.010.
Genome annotation databases
Ensembl	ENSMUSG00000004552. Mus musculus. [Contig view]
Organism-specific databases
MGI	MGI:107361. Ctse.
Phylogenomic databases
HOGENOM	P70269.
HOVERGEN	P70269.
Gene expression databases
ArrayExpress	P70269.
CleanEx	MM_CTSE.
GermOnline	ENSMUSG00000004552. Mus musculus.
Family and domain databases
InterPro	IPR001969. Pept_Asp_AS. IPR009007. Pept_Aspartc_cat. IPR001461. Peptidase_A1. IPR012848. Propep_A1. [Graphical view]
Gene3D	G3DSA:2.40.70.10. Pept_Aspartc_cat. 1 hit.
PANTHER	PTHR13683. Peptidase_A1. 1 hit.
Pfam	PF07966. A1_Propeptide. 1 hit. PF00026. Asp. 1 hit. [Graphical view]
PRINTS	PR00792. PEPSIN.
PROSITE	PS00141. ASP_PROTEASE. 2 hits. [Graphical view]
ProDom	P70269. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
SOURCE	Search...
ProtoNet	Search...

Entry information

Entry name

CATE_MOUSE

Accession

Primary (citable) accession number: P70269
Secondary accession number(s): O35647

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	July 22, 2008
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)