Reviewed, UniProtKB/Swiss-Prot P94523 (ARAA_BACSU)

Names and origin

Protein names

Recommended name:
    L-arabinose isomerase
    EC=5.3.1.4

Gene names

Name:	araA
Ordered Locus Names:	BSU28800

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	496 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the conversion of L-arabinose to L-ribulose By similarity.
Catalytic activity	L-arabinose = L-ribulose.
Cofactor	Binds 1 manganese ion per subunit By similarity.
Pathway	Carbohydrate degradation; L-arabinose degradation via L-ribulose; D-xylulose 5-phosphate from L-arabinose (bacteria route): step 1/3.
Induction	Transcription is repressed by glucose and by the binding of araR to the operon promoter. L-arabinose acts as an inducer by inhibiting the binding of araR to the DNA, thus allowing expression of the gene.
Sequence similarities	Belongs to the arabinose isomerase family.

Ontologies

Keywords
Biological process	Arabinose catabolism Carbohydrate metabolism
Ligand	Manganese Metal-binding
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arabinose catabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	L-arabinose isomerase activity Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 496	496	L-arabinose isomerase		PRO_0000198382
Sites
	Metal binding	305	1	Manganese By similarity
	Metal binding	330	1	Manganese By similarity
	Metal binding	347	1	Manganese By similarity
	Metal binding	446	1	Manganese By similarity
Experimental info
	Sequence conflict	85 – 94	10	AKMWIEGLSS → SQKLWKRRPFPP Ref.2 Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P94523-1 [UniParc]. Last modified January 23, 2002. Version 2. Checksum: E0DCF8B736364F18	FASTA	496	56,122
	10 20 30 40 50 60 MLQTKDYEFW FVTGSQHLYG EETLELVDQH AKSICEGLSG ISSRYKITHK PVVTSPETIR 70 80 90 100 110 120 ELLREAEYSE TCAGIITWMH TFSPAKMWIE GLSSYQKPLM HLHTQYNRDI PWGTIDMDFM 130 140 150 160 170 180 NSNQSAHGDR EYGYINSRMG LSRKVIAGYW DDEEVKKEMS QWMDTAAALN ESRHIKVARF 190 200 210 220 230 240 GDNMRHVAVT DGDKVGAHIQ FGWQVDGYGI GDLVEVMDRI TDDEVDTLYA EYDRLYVISE 250 260 270 280 290 300 ETKRDEAKVA SIKEQAKIEL GLTAFLEQGG YTAFTTSFEV LHGMKQLPGL AVQRLMEKGY 310 320 330 340 350 360 GFAGEGDWKT AALVRMMKIM AKGKRTSFME DYTYHFEPGN EMILGSHMLE VCPTVALDQP 370 380 390 400 410 420 KIEVHSLSIG GKEDPARLVF NGISGSAIQA SIVDIGGRFR LVLNEVNGQE IEKDMPNLPV 430 440 450 460 470 480 ARVLWKPEPS LKTAAEAWIL AGGAHHTCLS YELTAEQMLD WAEMAGIESV LISRDTTIHK 490 LKHELKWNEA LYRLQK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Bacillus subtilis L-arabinose (ara) operon: nucleotide sequence, genetic organization and expression." Sa-Nogueira I.M.G., Nogueira T.V., Soares S., de Lencastre H. Microbiology 143:957-969(1997) [PubMed: 9084180] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[2]	"The dnaB-pheA (256 degrees-240 degrees) region of the Bacillus subtilis chromosome containing genes responsible for stress responses, the utilization of plant cell walls and primary metabolism." Wipat A., Carter N., Brignell C.S., Guy J.B., Piper K., Sanders J., Emmerson P.T., Harwood C.R. Microbiology 142:3067-3078(1996) [PubMed: 8969504] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[3]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[4]	"Mode of action of AraR, the key regulator of L-arabinose metabolism in Bacillus subtilis." Mota L.J., Tavares P., Sa-Nogueira I.M.G. Mol. Microbiol. 33:476-489(1999) [PubMed: 10417639] [Abstract] Cited for: TRANSCRIPTIONAL REGULATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X89408 Genomic DNA. Translation: CAA61585.1. Z75208 Genomic DNA. Translation: CAA99587.1. Z99118 Genomic DNA. Translation: CAB14840.1.
PIR	C69587.
RefSeq	NP_390758.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	936764.
GenomeReviews	Gene locus BSU28800 in contig AL009126_GR.
KEGG	bsu:BSU28800.
Organism-specific databases
SubtiList	BG11904. araA. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	P94523.
Enzyme and pathway databases
BioCyc	BSUB224308:BSU2876-MON.
Family and domain databases
HAMAP	MF_00519. [Tree]
InterPro	IPR003762. Lara_isomerase. [Graphical view]
Pfam	PF02610. Arabinose_Isome. 1 hit. [Graphical view]
PIRSF	PIRSF001478. L-ara_isomerase. 1 hit.
ProDom	PD018364. Lara_isomerase. 1 hit. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
ProtoNet	Search...

Entry information

Entry name

ARAA_BACSU

Accession

Primary (citable) accession number: P94523
Secondary accession number(s): O05184

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	January 23, 2002
Last modified:	November 4, 2008
This is version 59 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)