Rho GTPase-activating protein 4 - Homo sapiens (Human)

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Reviewed, UniProtKB/Swiss-Prot P98171 (RHG04_HUMAN)

Last modified July 22, 2008. Version 81. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Rho GTPase-activating protein 4
Alternative name(s):
    Rho-type GTPase-activating protein 4
    Rho-GAP hematopoietic protein C1
    p115

Gene names

Name:	ARHGAP4
Synonyms:	KIAA0131, RGC1, RHOGAP4

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length	946 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Inhibitory effect on stress fiber organization. May down-regulate Rho-like GTPase in hematopoietic cells.
Subcellular location	Cytoplasm. Note= Just below the plasma membrane.
Tissue specificity	Predominantly in hematopoietic cells (spleen, thymus and leukocytes); low levels in placenta, lung and various fetal tissues.
Sequence similarities	Contains 1 FCH domain. Contains 1 Rho-GAP domain. Contains 1 SH3 domain.

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Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Polymorphism
Domain	Coiled coil SH3 domain
Molecular function	GTPase activation
PTM	Phosphoprotein
Technical term	3D-structure
Gene Ontology (GO)
Biological process	Rho protein signal transduction Ref.1 Traceable author statement. Source: ProtInc cytoskeleton organization and biogenesis Ref.1 Traceable author statement. Source: ProtInc
Cellular component	cytoplasm Ref.1 Traceable author statement. Source: ProtInc
Molecular function	Rho GTPase activator activity Ref.1 Traceable author statement. Source: ProtInc SH3/SH2 adaptor activity Ref.1 Traceable author statement. Source: ProtInc
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Molecule processing

Chain

1 – 946

946

Rho GTPase-activating protein 4

Regions

Domain

15 – 88

FCH

Domain

507 – 695

189

Rho-GAP

Domain

746 – 805

SH3

Coiled coil

128 – 195

Potential

Amino acid modifications

Modified residue

171

Phosphotyrosine

Natural variations

Natural variant

104

A → V: dbSNP rs5987182.

Experimental info

Sequence conflict

609

A → D in CAA55394. Ref.1

Sequence conflict

731

E → D in CAA55394. Ref.1

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Sequences

Sequence

Length

Mass (Da)

Tools

P98171-1 [UniParc].

Last modified October 17, 2006. Version 2.
Checksum: ED3D48C5DAA24969
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FASTA

946

105,026

        10         20         30         40         50         60 
MAAHGKLRRE RGLQAEYETQ VKEMRWQLSE QLRCLELQGE LRRELLQELA EFMRRRAEVE 

        70         80         90        100        110        120 
LEYSRGLEKL AERFSSRGGR LGSSREHQSF RKEPSLLSPL HCWAVLLQHT RQQSRESAAL 

       130        140        150        160        170        180 
SEVLAGPLAQ RLSHIAEDVG RLVKKSRDLE QQLQDELLEV VSELQTAKKT YQAYHMESVN 

       190        200        210        220        230        240 
AEAKLREAER QEEKRAGRSV PTTTAGATEA GPLRKSSLKK GGRLVEKRQA KFMEHKLKCT 

       250        260        270        280        290        300 
KARNEYLLSL ASVNAAVSNY YLHDVLDLMD CCDTGFHLAL GQVLRSYTAA ESRTQASQVQ 

       310        320        330        340        350        360 
GLGSLEEAVE ALDPPGDKAK VLEVHATVFC PPLRFDYHPH DGDEVAEICV EMELRDEILP 

       370        380        390        400        410        420 
RAQNIQSRLD RQTIETEEVN KTLKATLQAL LEVVASDDGD VLDSFQTSPS TESLKSTSSD 

       430        440        450        460        470        480 
PGSRQAGRRR GQQQETETFY LTKLQEYLSG RSILAKLQAK HEKLQEALQR GDKEEQEVSW 

       490        500        510        520        530        540 
TQYTQRKFQK SRQPRPSSQY NQRLFGGDME KFIQSSGQPV PLVVESCIRF INLNGLQHEG 

       550        560        570        580        590        600 
IFRVSGAQLR VSEIRDAFER GEDPLVEGCT AHDLDSVAGV LKLYFRSLEP PLFPPDLFGE 

       610        620        630        640        650        660 
LLASSELEAT AERVEHVSRL LWRLPAPVLV VLRYLFTFLN HLAQYSDENM MDPYNLAVCF 

       670        680        690        700        710        720 
GPTLLPVPAG QDPVALQGRV NQLVQTLIVQ PDRVFPPLTS LPGPVYEKCM APPSASCLGD 

       730        740        750        760        770        780 
AQLESLGADN EPELEAEMPA QEDDLEGVVE AVACFAYTGR TAQELSFRRG DVLRLHERAS 

       790        800        810        820        830        840 
SDWWRGEHNG MRGLIPHKYI TLPAGTEKQV VGAGLQTAGE SGSSPEGLLA SELVHRPEPC 

       850        860        870        880        890        900 
TSPEAMGPSG HRRRCLVPAS PEQHVEVDKA VAQNMDSVFK ELLGKTSVRQ GLGPASTTSP 

       910        920        930        940 
SPGPRSPKAP PSSRLGRNKG FSRGPGAPAS PSASHPQGLD TTPKPH

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"An X chromosome-linked gene encoding a protein with characteristics of a rhoGAP predominantly expressed in hematopoietic cells." Tribioli C., Droetto S., Bione S., Cesareni G., Torrisi M.R., Lotti L.V., Lanfrancone L., Toniolo D., Pelicci P.-G. Proc. Natl. Acad. Sci. U.S.A. 93:695-699(1996) [PubMed: 8570618] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Embryo.
[2]	"Prediction of the coding sequences of unidentified human genes. IV. The coding sequences of 40 new genes (KIAA0121-KIAA0160) deduced by analysis of cDNA clones from human cell line KG-1." Nagase T., Seki N., Tanaka A., Ishikawa K., Nomura N. DNA Res. 2:167-174(1995) [PubMed: 8590280] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 5-946. Tissue: Bone marrow.
[3]	"Isolation of new genes in distal Xq28: transcriptional map and identification of a human homologue of the ARD1 N-acetyl transferase of Saccharomyces cerevisiae." Tribioli C., Mancini M., Plassart E., Bione S., Rivella S., Sala C., Torri G., Toniolo D. Hum. Mol. Genet. 3:1061-1068(1994) [PubMed: 7981673] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-103. Tissue: Embryo.
[4]	"Tyrosine phosphorylated Par3 regulates epithelial tight junction assembly promoted by EGFR signaling." Wang Y., Du D., Fang L., Yang G., Zhang C., Zeng R., Ullrich A., Lottspeich F., Chen Z. EMBO J. 25:5058-5070(2006) [PubMed: 17053785] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-171, MASS SPECTROMETRY.
[5]	"Solution structure of SH3 domain in Rho-GTPase-activating protein 4." RIKEN structural genomics initiative (RSGI) Submitted (OCT-2007) to the PDB data bank Cited for: STRUCTURE BY NMR OF 746-814.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

X78817 mRNA. Translation: CAA55394.1.
D50921 mRNA. Translation: BAA09480.1.

PIR

I38100.

RefSeq

NP_001657.3.

UniGene

Hs.433291
Hs.522818

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2EPD	NMR	-	A	746-814	[»]

ModBase

Search...

PTM databases

PhosphoSite

P98171.

Genome annotation databases

Ensembl

ENSG00000089820. Homo sapiens. [Contig view]

GeneID

393.

KEGG

hsa:393.

Organism-specific databases

H-InvDB

HIX0017142.

HGNC

HGNC:674. ARHGAP4.

HPA

HPA001012.
HPA001083.

MIM

300023. gene.

PharmGKB

PA24958.

HUGE

Search...

GenAtlas

Search...

GeneCards

Search...

Phylogenomic databases

HOVERGEN

P98171.

Enzyme and pathway databases

Reactome

REACT_11044. Signaling by Rho GTPases.

Gene expression databases

CleanEx

HS_ARHGAP4.

GermOnline

ENSG00000089820. Homo sapiens.

Family and domain databases

InterPro

IPR001060. Cdc15_Fes_CIP4.
IPR000198. RhoGAP.
IPR001452. SH3.
[Graphical view]

Gene3D

G3DSA:1.10.555.10. RhoGAP. 1 hit.

Pfam

PF00611. FCH. 1 hit.
PF00620. RhoGAP. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]

ProDom

PD000066. SH3. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00055. FCH. 1 hit.
SM00324. RhoGAP. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]

PROSITE

PS50133. FCH. 1 hit.
PS50238. RHOGAP. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

BLOCKS

Search...

Other Resources

SOURCE

Search...

ProtoNet

Search...

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Entry information

Entry name

RHG04_HUMAN

Accession

Primary (citable) accession number: P98171
Secondary accession number(s): Q14144

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 17, 2006
Last modified:	July 22, 2008
This is version 81 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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