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Reviewed, UniProtKB/Swiss-Prot Q9GPM4 (PGK_DICDI)

Last modified July 22, 2008. Version 38. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Phosphoglycerate kinase
    EC=2.7.2.3
Gene names
Name: pgkA
ORF Names: DDB_0191349
OrganismDictyostelium discoideum (Slime mold) [Complete proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryota › Mycetozoa › Dictyosteliida › Dictyostelium

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Protein attributes

Sequence length420 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Catalytic activity

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5.

Subunit structure

Monomer. Interacts with calmodulin in the presence of Ca(2+).

Subcellular location

CytoplasmBy similarity.

Developmental stage

Expressed during development. Expression levels decrease steadily from the initiation of development until culmination. Levels increase after 18 hours of development and peak at 22 hours, after which they decrease again.

Sequence similarities

Belongs to the phosphoglycerate kinase family.

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Ontologies

Keywords

   Biological processGlycolysis
   Cellular componentCytoplasm
   LigandATP-binding
Calmodulin-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   PTMPhosphoprotein
   Technical termComplete proteome

Gene Ontology (GO)

None. [Check GOA]

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical view

Molecule processing

Chain1 – 420420Phosphoglycerate kinase

Regions

Nucleotide binding376 – 3794ATP By similarity
Region27 – 293Substrate binding By similarity
Region67 – 704Substrate binding By similarity
Region209 – 22820Calmodulin binding

Sites

Binding site431Substrate By similarity
Binding site1261Substrate By similarity
Binding site1741Substrate By similarity
Binding site2231ATP By similarity
Binding site3161ATP; via carbonyl oxygen By similarity
Binding site3471ATP By similarity

Amino acid modifications

Modified residue1991Phosphotyrosine By similarity
Modified residue2061Phosphoserine By similarity
Modified residue3931Phosphoserine By similarity

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Sequences

Sequence LengthMass (Da)Tools
Q9GPM4-1 [UniParc].

Last modified October 1, 2002. Version 2.
Checksum: 6FEA000CF9B5F83E

FASTA42045,709
        10         20         30         40         50         60 
MSSNKIGNKL SLDKVDVKGK RVLIRVDYNV PLDKNNNITS TQRIDASIPT LEYCLKNGAK 

        70         80         90        100        110        120 
SIVLMSHLGR PDGLVKPEYS LKPVVKVLED QLKRPIKFLS DCVGEQVEKE CANPEEGTVI 

       130        140        150        160        170        180 
LLENLRFHIE EEGSGVDAEG KKVKANPEKV KEFRESLTKL GDVYVNDAFG TAHRAHSSMV 

       190        200        210        220        230        240 
GINLPQKAAG FLMKKELEYF AKALESPSKP FLAILGGAKV SDKIKLIENL LYKVDEMIIG 

       250        260        270        280        290        300 
GGMAFTFKKF IDNKEIGSSL FEKTAEQITK DIIAKAAKNN VKLHFPVDYV IADKFDNDAN 

       310        320        330        340        350        360 
IKTVTQDQGI PEGWMGLDCG PETIKENRDT ISRAKTIVWN GPMGVFEKSN FEAGTKAAMD 

       370        380        390        400        410        420 
DVVNATTNGA ITIIGGGDTA TCAAKYNTED KVSHVSTGGG ASLELLEGKE LPGVTALSDL

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References

« Hide 'large scale' references
[1]"Calmodulin binds to and inhibits the activity of phosphoglycerate kinase."
Myre M.A., O'Day D.H.
Biochim. Biophys. Acta 1693:177-183(2004) [PubMed: 15363631] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH CALMODULIN.
[2]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed: 15875012] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[3]"A transcriptional profile of multicellular development in Dictyostelium discoideum."
Van Driessche N., Shaw C., Katoh M., Morio T., Sucgang R., Ibarra M., Kuwayama H., Saito T., Urushihara H., Maeda M., Takeuchi I., Ochiai H., Eaton W., Tollett J., Halter J., Kuspa A., Tanaka Y., Shaulsky G.
Development 129:1543-1552(2002) [PubMed: 11923193] [Abstract]
Cited for: DEVELOPMENTAL STAGE.
[4]"Genome-wide expression analyses of gene regulation during early development of Dictyostelium discoideum."
Iranfar N., Fuller D., Loomis W.F.
Eukaryot. Cell 2:664-670(2003) [PubMed: 12912885] [Abstract]
Cited for: DEVELOPMENTAL STAGE.
[5]"Proteomics fingerprinting of phagosome maturation and evidence for the role of a Galpha during uptake."
Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M., Soldati T.
Mol. Cell. Proteomics 5:2228-2243(2006) [PubMed: 16926386] [Abstract]
Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.

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Cross-references

Sequence databases

AF316577 mRNA. Translation: AAG34561.2.
AAFI02000103 Genomic DNA. Translation: EAL63606.1.
RefSeqXP_637130.1.

3D structure databases

HSSPHSSP built from PDB template 1FW8 based on UniProtKB P00560.
ModBaseSearch...

Genome annotation databases

GeneID3388699.
KEGGddi:DDB_0191349.

Organism-specific databases

dictyBaseDDB0191349. pgkA.

Family and domain databases

InterProIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
Gene3DG3DSA:3.40.50.1270. Phosphoglycerate_kinase_C. 1 hit.
G3DSA:3.40.50.1260. Phosphoglycerate_kinase_N. 1 hit.
PANTHERPTHR11406. PGK. 1 hit.
PfamPF00162. PGK. 1 hit.
[Graphical view]
PRINTSPR00477. PHGLYCKINASE.
PROSITEPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]
ProDomQ9GPM4.
[Graphical view] [Entries sharing at least one domain]
BLOCKSSearch...

Other Resources

ProtoNetSearch...

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Entry information

Entry namePGK_DICDI
AccessionPrimary (citable) accession number: Q9GPM4
Secondary accession number(s): Q54K34
Entry history
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: October 1, 2002
Last modified: July 22, 2008
This is version 38 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

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Relevant documents

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase

PATHWAY comments

Index of metabolic and biosynthesis pathways

UniProtKB secondary accession numbers

Index of UniProtKB secondary accession numbers

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents