Reviewed, UniProtKB/Swiss-Prot Q27563 (PSA3_DICDI)

Names and origin

Protein names

Recommended name:
    Proteasome subunit alpha type-3
    EC=3.4.25.1

Gene names

Name:	psmA3
Synonyms:	prtD
ORF Names:	DDB_0191264

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	248 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.
Catalytic activity	Cleavage of peptide bonds with very broad specificity.
Subunit structure	The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel By similarity.
Subcellular location	CytoplasmBy similarity. NucleusBy similarity.
Sequence similarities	Belongs to the peptidase T1A family.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus Proteasome
Molecular function	Hydrolase Protease Threonine protease
Technical term	Complete proteome
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 248	248	Proteasome subunit alpha type-3
Experimental info
	Sequence conflict	178 – 180	3	KIK → EIR in AAB03671. Ref.1
	Sequence conflict	221	1	N → D in AAB03671. Ref.1
	Sequence conflict	235	1	A → G in AAB03671. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q27563-1 [UniParc]. Last modified December 4, 2007. Version 2. Checksum: F6BAAE2666948CBB	FASTA	248	27,238
	10 20 30 40 50 60 MSSVGSGYDL YVSTYSPDGK LFQVDYANKA VENSGTLVAI KAKDGVVLGV EKLVPSKMLC 70 80 90 100 110 120 SGSNRRVHHI DTHVGVAIAG FLADARQLIT RARSEAKNYK STYGQPIPIK VLSQRIASYN 130 140 150 160 170 180 HMHTLYGSVR PFGCSIAITG IDQYGPQLFL VEPSGSCVGY FGASLGKGKQ AAKNELEKIK 190 200 210 220 230 240 FSEMTCREAI KEVSRIIYSV HDEVKDKDFE LELGWISTET NNVHQIVPKE LHDEAEAYAK QSLEDANM

References

[1]

"Ordered yeast artificial chromosome clones representing the Dictyostelium discoideum genome." Kuspa A., Loomis W.F. Proc. Natl. Acad. Sci. U.S.A. 93:5562-5566(1996) [PubMed: 8643615] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: AX4.

[2]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U61988 mRNA. Translation: AAB03671.1. AAFI02000003 Genomic DNA. Translation: EAL73156.1.
RefSeq	XP_647103.1.
3D structure databases
HSSP	HSSP built from PDB template 1RYP based on UniProtKB P21242.
ModBase	Search...
Protein family/group databases
MEROPS	T01.977.
Genome annotation databases
GeneID	3398811.
KEGG	ddi:DDB_0191264.
Organism-specific databases
dictyBase	DDB0191264. psmA3.
Family and domain databases
InterPro	IPR001353. Proteasome_A_B_su. IPR000426. Proteasome_alpha_CS. [Graphical view]
Pfam	PF00227. Proteasome. 1 hit. [Graphical view]
PROSITE	PS00388. PROTEASOME_A. 1 hit. [Graphical view]
ProDom	Q27563. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

PSA3_DICDI

Accession

Primary (citable) accession number: Q27563
Secondary accession number(s): Q55GT1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	December 4, 2007
Last modified:	September 23, 2008
This is version 55 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)