Reviewed, UniProtKB/Swiss-Prot P21900 (PURA_DICDI)

Names and origin

Protein names

Recommended name:
    Adenylosuccinate synthetase
      Short name(s)=AdSS
    EC=6.3.4.4
Alternative name(s):
    IMP--aspartate ligase
    AMPSase

Gene names

Name:	purA
ORF Names:	DDB_0201565

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	427 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Plays an important role in the de novo pathway of purine nucleotide biosynthesis.
Catalytic activity	GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP.
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2.
Subunit structure	Homodimer By similarity.
Subcellular location	CytoplasmBy similarity.
Developmental stage	Seen during growth but not during development.
Sequence similarities	Belongs to the adenylosuccinate synthetase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 427	427	Adenylosuccinate synthetase
Regions
	Nucleotide binding	11 – 17	7	GTP Potential
Sites
	Active site	143	1	By similarity
	Active site	150	1	By similarity
	Metal binding	12	1	Magnesium By similarity
	Metal binding	39	1	Magnesium (via carbonyl oxygen) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P21900-1 [UniParc]. Last modified May 1, 1991. Version 1. Checksum: D716A28D5D465767	FASTA	427	47,285
	10 20 30 40 50 60 MASIIIGSQW GDEGKGKLVD ILSQQFDVVA RCQGGANAGH TIVVDGKKIA LHLIPSGILN 70 80 90 100 110 120 EKASCILGNG MVIHLPTFFK EVQGLQDKGI NYKGRLFVSD RAHLVFDLHQ MIDAMKEAEL 130 140 150 160 170 180 SNGTSNDSIG TTKRGIGPCY SSKASRGGLR VCDLYSPEHF RKTFTRLVEN KHKRFGSFEY 190 200 210 220 230 240 DVEAELKRYQ EFAEMLKPFV IDSVYYLNQA FKDGKKVLIE GAQSTMLDLD FGCYPYVTSS 250 260 270 280 290 300 ASSVGGACTG LGISPNKVVT QIGVVKAYTT KVGSGPFPTE QNDHVGDSLR KAGSEFGTTT 310 320 330 340 350 360 GRPRRIGWLD AVVLRYTSMI NDFTRLNLTK LDVLSDFEEI KIGVDYKYKG ETIKSFPASL 370 380 390 400 410 420 ETLAQCEVVY ESFPGWKCDL SHVTEYDQLP IQAKNYIKRI EELVGVPIVY IGVGVERKNL IERKELI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Purification and cDNA-derived sequence of adenylosuccinate synthetase from Dictyostelium discoideum." Wiesmueller L., Wittbrodt J., Noegel A.A., Schleicher M. J. Biol. Chem. 266:2480-2485(1991) [PubMed: 1989999] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[3]	"Proteomics fingerprinting of phagosome maturation and evidence for the role of a Galpha during uptake." Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M., Soldati T. Mol. Cell. Proteomics 5:2228-2243(2006) [PubMed: 16926386] [Abstract] Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M58471 mRNA. Translation: AAA33167.1. AAFI02000079 Genomic DNA. Translation: EAL64552.1.
PIR	AJDODS. A38617.
RefSeq	XP_638113.1.
3D structure databases
HSSP	HSSP built from PDB template 1DJ3 based on UniProtKB O24396.
ModBase	Search...
Genome annotation databases
GeneID	3389800.
KEGG	ddi:DDB_0201565.
Organism-specific databases
dictyBase	DDB0201565. purA.
Family and domain databases
InterPro	IPR001114. AdlSucc_Synth. [Graphical view]
PANTHER	PTHR11846. Asucc_synthtase. 1 hit.
Pfam	PF00709. Adenylsucc_synt. 1 hit. [Graphical view]
ProDom	PD001188. Asucc_synthtase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00788. Adenylsucc_synt. 1 hit. [Graphical view]
TIGRFAMs	TIGR00184. purA. 1 hit.
PROSITE	PS01266. ADENYLOSUCCIN_SYN_1. 1 hit. PS00513. ADENYLOSUCCIN_SYN_2. 1 hit. [Graphical view]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

PURA_DICDI

Accession

Primary (citable) accession number: P21900
Secondary accession number(s): Q54N30

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	May 1, 1991
Last modified:	July 22, 2008
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)