Reviewed, UniProtKB/Swiss-Prot Q01294 (CARP_NEUCR)

Names and origin

Protein names

Recommended name:
    Vacuolar protease A
    EC=3.4.23.-

Gene names

Name:	pep-4
ORF Names:	B13D24.090, NCU02273

Organism

Neurospora crassa [Complete proteome]

Taxonomic identifier

5141 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Neurospora

Protein attributes

Sequence length	396 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Subcellular location	Vacuole. Note= Lysosome-like vacuoles Potential.
Sequence similarities	Belongs to the peptidase A1 family.

Ontologies

Keywords
Cellular component	Vacuole
Domain	Signal
Molecular function	Aspartyl protease Hydrolase Protease
PTM	Glycoprotein Zymogen
Technical term	Complete proteome
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Propeptide	18 – 70	53	Activation peptide
	Chain	71 – 396	326	Vacuolar protease A
Sites
	Active site	103	1	By similarity
	Active site	284	1	By similarity
Amino acid modifications
	Glycosylation	138	1	N-linked (GlcNAc...) Potential
	Glycosylation	335	1	N-linked (GlcNAc...) Potential
	Disulfide bond	116 ↔ 121		By similarity
	Disulfide bond	318 ↔ 351		By similarity
Experimental info
	Sequence conflict	31 – 32	2	EQ → DE in AAA79878. Ref.1
	Sequence conflict	199	1	I → L in AAA79878. Ref.1
	Sequence conflict	202	1	N → P in AAA79878. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q01294-1 [UniParc]. Last modified February 15, 2005. Version 2. Checksum: 3498032A1E703195	FASTA	396	42,933
	10 20 30 40 50 60 MKGALLTAAM LLGSAQAGVH TMKLKKVPLA EQLESVPIDV QVQHLGQKYT GLRTESHTQA 70 80 90 100 110 120 MFKATDAQVS GNHPVPITNF MNAQYFSEIT IGTPPQTFKV VLDTGSSNLW VPSSQCGSIA 130 140 150 160 170 180 CYLHNKYESS ESSTYKKNGT SFKIEYGSGS LSGFVSQDRM TIGDITINDQ LFAEATSEPG 190 200 210 220 230 240 LAFAFGRFDG ILGLGYDRIA VNGITPPFYK MVEQKLVDEP VFSFYLADQD GESEVVFGGV 250 260 270 280 290 300 NKDRYTGKIT TIPLRRKAYW EVDFDAIGYG KDFAELEGHG VILDTGTSLI ALPSQLAEML 310 320 330 340 350 360 NAQIGAKKSW NGQFTIDCGK KSSLEDVTFT LAGYNFTLGP EDYILEASGS CLSTFMGMDM 370 380 390 PAPVGPLAIL GDAFLRKYYS IYDLGADTVG IATAKR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of a vacuolar protease in Neurospora crassa and the use of gene RIPing to generate protease-deficient strains." Vazquez-Laslop N., Tenney K., Bowman B.J. J. Biol. Chem. 271:21944-21949(1996) [PubMed: 8702999] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"What's in the genome of a filamentous fungus? Analysis of the Neurospora genome sequence." Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U. Nucleic Acids Res. 31:1944-1954(2003) [PubMed: 12655011] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[3]	"The genome sequence of the filamentous fungus Neurospora crassa." Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W. Nature 422:859-868(2003) [PubMed: 12712197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U36471 Genomic DNA. Translation: AAA79878.1. BX908789 Genomic DNA. Translation: CAF05874.1. AABX02000010 Genomic DNA. Translation: EAA30681.1.
PIR	T47207.
RefSeq	XP_959917.1.
3D structure databases
HSSP	HSSP built from PDB template 2REN based on UniProtKB P00797.
ModBase	Search...
Protein family/group databases
MEROPS	A01.018.
Genome annotation databases
GeneID	3876039.
KEGG	ncr:NCU02273.
NMPDR	fig|5141.1.peg.8982.
Family and domain databases
InterPro	IPR001969. Pept_Asp_AS. IPR009007. Pept_Aspartc_cat. IPR001461. Peptidase_A1. [Graphical view]
Gene3D	G3DSA:2.40.70.10. Pept_Aspartc_cat. 1 hit.
PANTHER	PTHR13683. Peptidase_A1. 1 hit.
Pfam	PF00026. Asp. 1 hit. [Graphical view]
PRINTS	PR00792. PEPSIN.
PROSITE	PS00141. ASP_PROTEASE. 2 hits. [Graphical view]
ProDom	Q01294. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

CARP_NEUCR

Accession

Primary (citable) accession number: Q01294
Secondary accession number(s): Q7RVB0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 15, 2005
Last modified:	September 2, 2008
This is version 58 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)