Reviewed, UniProtKB/Swiss-Prot Q02773 (RPM2_YEAST)

Names and origin

Protein names

Recommended name:
    Ribonuclease P protein component, mitochondrial
      Short name(s)=RNase P
    EC=3.1.26.5

Gene names

Name:	RPM2
Ordered Locus Names:	YML091C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	1202 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Ribonuclease P generates mature tRNA molecules by cleaving their 5'-ends.
Catalytic activity	Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.
Subunit structure	Consists of a RNA moiety (RPM1) and the protein component (RPM2). Both are necessary for full enzymatic activity.
Subcellular location	Mitochondrion.
Miscellaneous	Present with 1160 molecules/cell in log phase SD medium.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Mitochondrion
Domain	Transit peptide
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	mRNA processing Inferred from genetic interaction. Source: SGD mitochondrion organization and biogenesis Inferred from genetic interaction. Source: SGD positive regulation of transcription from RNA polymerase II promoter Inferred from direct assay. Source: SGD tRNA 5'-leader removal Inferred from direct assay. Source: SGD translation Inferred from mutant phenotype. Source: SGD
Cellular component	mitochondrial ribonuclease P complex Inferred from direct assay. Source: SGD nucleus Inferred from direct assay. Source: SGD
Molecular function	transcription activator activity Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Transit peptide	1 – 122	122	Mitochondrion
	Chain	123 – 1202	1080	Ribonuclease P protein component, mitochondrial

Sequences

	Sequence		Length	Mass (Da)
	Q02773-1 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: B9DA65450E8F056A	FASTA	1,202	139,348
	10 20 30 40 50 60 MAFKSFIYSK GYHRSAAQKK TATSFFDSSY QYLRQNQGLV NSDPVLHASH LHPHPVVVAN 70 80 90 100 110 120 VNYNNVDDIL HPHDLDSSIN NTNNPLTHEE LLYNQNVSLR SLKQQQSTNY VNNNNNNQHR 130 140 150 160 170 180 YYSTGPTLPT NQYDPLNFSN RNFQDLSLKT SQPSVQQPQN EYSLLKDENA PVWKEDTEPC 190 200 210 220 230 240 LNKSTYLQTH IDEINRCYEQ KNYNKINSLY QSLKRNDIVP PLEIFTKVLD SLCKRPLDNN 250 260 270 280 290 300 DLDNKMYELL TCYQDMINNR LKPPDEIYNI VLLSLFKGSI LAYQFENPNG SDFYKIAIEL 310 320 330 340 350 360 FNTTTNDPKQ KSVVKFRNFS KDVLDYNLLA MNIYPGHITL SKAQQVIKSS PAFIKDSFYF 370 380 390 400 410 420 IACFSYAKLT NDKFAIKELY EDFRLSLSSG SPDQGLFDDQ FEIYSVILSS FIETGEVELA 430 440 450 460 470 480 TNLLDDLVSK IQSSNGLASN ISLLLSSFLI SMSKVDPSKA YEIWFKFHNL NWIPEFSYEF 490 500 510 520 530 540 YLVFMANSFQ DWNLTKKIYD YIFPMERNLS PLKKQKLSDY LLHPIGVDSI TTSLLDYSLQ 550 560 570 580 590 600 LKDNEVIMKI LEESIVKNFS FDIGIYPFVF NYLREIQCGE DYLMRFIESH AEFIKKSNSI 610 620 630 640 650 660 NKFQFLNMIV DNFQSQSLLN KISHAKFFKN FVEDFNLENC ELVSYNGLIS CINNFIKIPK 670 680 690 700 710 720 TIKDFPYILE IHAILVTKLF DFDTYPILQN GNNEVLLKFR DQIEHQFKML AQNFCRLNLD 730 740 750 760 770 780 PNLLAGVVSQ AMKMVNLDDT ANGQDLLNFF NHPGDWDKSY PLSLGSFIRN SPRGGIREFT 790 800 810 820 830 840 KLSKEGYCFD YDTYKELIIK RAINKQIIDK CLEVCPDSIE LKNIVNLMIS KIPGRNLTQL 850 860 870 880 890 900 IINNPKFSKV FVPNLRNDSM LKLIENCESL SNFIRICDFP EKFKSIAIQA ENKNAIELIY 910 920 930 940 950 960 ERLFDGGKYA DILRYNNIVP VLNLELLLKS CIRSGEFKKY ESLSKKFNDK ISESSKIDIQ 970 980 990 1000 1010 1020 LEYLINKNDL KGAFTLFEKT PRELRTPHKT MDLYTFALFL DSFNRNITYY ESPENTLQFA 1030 1040 1050 1060 1070 1080 NILSSQTSFI NLLSTYNLIA HSDHLMNFNV GGMAAKVKKE ILNQMLNNLY DSIRLLSPSI 1090 1100 1110 1120 1130 1140 ENDKSMKEKL REKVKNYCRF KAYLKSPELD MDELKTLVSV ESFLNPFTPS MLFNNLIETI 1150 1160 1170 1180 1190 1200 YINEHASSLV LQNGLIYSLQ QKGLNKILSY LEESFITSGN DANIEKVREF RSLLRKSKPL QA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A 105-kDa protein is required for yeast mitochondrial RNase P activity." Morales M.J., Dang Y.L., Lou Y.C., Sulo P., Martin N.C. Proc. Natl. Acad. Sci. U.S.A. 89:9875-9879(1992) [PubMed: 1409716] [Abstract] Cited for: NUCLEOTIDE SEQUENCE, PROTEIN SEQUENCE OF 123-142.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBLGenBankDDBJ	L06209 Unassigned DNA. Translation: AAA03168.1. Z46660 Genomic DNA. Translation: CAA86647.1.
PIR	A48773.
RefSeq	NP_013619.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:6320N.
IntAct	Q02773.
Proteomic databases
PeptideAtlas	Q02773.
Genome annotation databases
Ensembl	YML091C. Saccharomyces cerevisiae. [Contig view]
GeneID	854883.
GenomeReviews	Gene locus YML091C in contig Z71257_GR.
KEGG	sce:YML091C.
NMPDR	fig|4932.3.peg.4656.
Organism-specific databases
CYGD	YML091c.
SGD	S000004556. RPM2.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	Q02773.
Gene expression databases
ArrayExpress	Q02773.
GermOnline	YML091C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013888. RNase_P_Rpm2_mt. [Graphical view]
Pfam	PF08579. RPM2. 1 hit. [Graphical view]
ProDom	Q02773. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
LinkHub	Q02773.
ProtoNet	Search...

Entry information

Entry name

RPM2_YEAST

Accession

Primary (citable) accession number: Q02773

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	July 22, 2008
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)