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Reviewed, UniProtKB/Swiss-Prot Q02793 (SKI8_YEAST)

Last modified July 22, 2008. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Antiviral protein SKI8
Alternative name(s):
    Superkiller protein 8
Gene names
Name: SKI8
Synonyms: REC103
Ordered Locus Names: YGL213C
OrganismSaccharomyces cerevisiae (Baker's yeast) [Complete proteome]
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length397 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Involved in double-strand break (DSB) formation during meiotic recombination through stabilization of SPO11 association with meiotic chromosome and helping SPO11 to recruit other DSB proteins like REC102 and REC104 to meiotic chromosomes. Also component of the SKI complex involved in 3'-mRNA degradation pathway. Represses dsRNA virus propagation by specifically blocking translation of viral mRNAs, perhaps recognizing the absence of CAP or poly(A). Essential for controlling the propagation of M double-stranded RNA (dsRNA) and thus for preventing virus-induced cytopathology.

Subunit structure

Component of the SKI complex composed of at least SKI2, SKI3 and SKI8. The SKI complex interacts with SKI7, which makes the link between the SKI complex and the exosome in order to perform mRNA degradation.

Subcellular location

Cytoplasm. Nucleus. Note= Relocalizes from the cytoplasm to the nucleus and is recruited to chromosomes by SPO11 during meiotic prophase.

Miscellaneous

Present with 1710 molecules/cell in log phase SD medium.

Sequence similarities

Contains 7 WD repeats.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical view

Molecule processing

Chain1 – 397397Antiviral protein SKI8

Regions

Repeat21 – 4222WD 1
Repeat67 – 10135WD 2
Repeat126 – 15732WD 3
Repeat191 – 21323WD 4
Repeat238 – 26427WD 5
Repeat296 – 31924WD 6
Repeat359 – 39234WD 7

Secondary structure

1 ......................................................................... 397
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
Q02793-1 [UniParc].

Last modified July 1, 1993. Version 1.
Checksum: C5E7B9D2268F9727

FASTA39744,232
        10         20         30         40         50         60 
MSKVFIATAN AGKAHDADIF SVSACNSFTV SCSGDGYLKV WDNKLLDNEN PKDKSYSHFV 

        70         80         90        100        110        120 
HKSGLHHVDV LQAIERDAFE LCLVATTSFS GDLLFYRITR EDETKKVIFE KLDLLDSDMK 

       130        140        150        160        170        180 
KHSFWALKWG ASNDRLLSHR LVATDVKGTT YIWKFHPFAD ESNSLTLNWS PTLELQGTVE 

       190        200        210        220        230        240 
SPMTPSQFAT SVDISERGLI ATGFNNGTVQ ISELSTLRPL YNFESQHSMI NNSNSIRSVK 

       250        260        270        280        290        300 
FSPQGSLLAI AHDSNSFGCI TLYETEFGER IGSLSVPTHS SQASLGEFAH SSWVMSLSFN 

       310        320        330        340        350        360 
DSGETLCSAG WDGKLRFWDV KTKERITTLN MHCDDIEIEE DILAVDEHGD SLAEPGVFDV 

       370        380        390 
KFLKKGWRSG MGADLNESLC CVCLDRSIRW FREAGGK

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References

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[1]"A yeast antiviral protein, SKI8, shares a repeated amino acid sequence pattern with beta-subunits of G proteins and several other proteins."
Matsumoto Y., Sarkar G., Sommer S.S., Wickner R.B.
Yeast 9:43-51(1993) [PubMed: 8442386] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Analysis of 21.7 kb DNA sequence from the left arm of chromosome VII reveals 11 open reading frames: two correspond to new genes."
Feuermann M., Simeonava L., Souciet J.-L., Potier S.
Yeast 13:475-477(1997) [PubMed: 9153757] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed: 9169869] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[4]"Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII."
Rieger M., Brueckner M., Schaefer M., Mueller-Auer S.
Yeast 13:1077-1090(1997) [PubMed: 9290212] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-245.
Strain: ATCC 204508 / S288c.
[5]"Superkiller mutations in Saccharomyces cerevisiae suppress exclusion of M2 double-stranded RNA by L-A-HN and confer cold sensitivity in the presence of M and L-A-HN."
Ridley S.P., Sommer S.S., Wickner R.B.
Mol. Cell. Biol. 4:761-770(1984) [PubMed: 6371496] [Abstract]
Cited for: FUNCTION.
[6]"Gene disruption indicates that the only essential function of the SKI8 chromosomal gene is to protect Saccharomyces cerevisiae from viral cytopathology."
Sommer S.S., Wickner R.B.
Virology 157:252-256(1987) [PubMed: 3029964] [Abstract]
Cited for: FUNCTION.
[7]"Decoying the cap- mRNA degradation system by a double-stranded RNA virus and poly(A)- mRNA surveillance by a yeast antiviral system."
Masison D.C., Blanc A., Ribas J.C., Carroll K., Sonenberg N., Wickner R.B.
Mol. Cell. Biol. 15:2763-2771(1995) [PubMed: 7739557] [Abstract]
Cited for: FUNCTION.
[8]"Molecular and genetic analysis of REC103, an early meiotic recombination gene in yeast."
Gardiner J.M., Bullard S.A., Chrome C., Malone R.E.
Genetics 146:1265-1274(1997) [PubMed: 9258672] [Abstract]
Cited for: FUNCTION.
[9]"The 3' to 5' degradation of yeast mRNAs is a general mechanism for mRNA turnover that requires the SKI2 DEVH box protein and 3' to 5' exonucleases of the exosome complex."
Anderson J.S.J., Parker R.
EMBO J. 17:1497-1506(1998) [PubMed: 9482746] [Abstract]
Cited for: FUNCTION.
[10]"The yeast antiviral proteins Ski2p, Ski3p, and Ski8p exist as a complex in vivo."
Brown J.T., Bai X., Johnson A.W.
RNA 6:449-457(2000) [PubMed: 10744028] [Abstract]
Cited for: FUNCTION, IDENTIFICATION IN THE SKI COMPLEX.
[11]"Ski7p G protein interacts with the exosome and the Ski complex for 3'-to-5' mRNA decay in yeast."
Araki Y., Takahashi S., Kobayashi T., Kajiho H., Hoshino S., Katada T.
EMBO J. 20:4684-4693(2001) [PubMed: 11532933] [Abstract]
Cited for: INTERACTION WITH SKI7, FUNCTION OF THE SKI COMPLEX.
[12]"A cis-acting element known to block 3' mRNA degradation enhances expression of polyA-minus mRNA in wild-type yeast cells and phenocopies a ski mutant."
Brown J.T., Johnson A.W.
RNA 7:1566-1577(2001) [PubMed: 11720286] [Abstract]
Cited for: FUNCTION OF THE SKI COMPLEX.
[13]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed: 14562095] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[14]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[15]"Systematic, genome-wide identification of host genes affecting replication of a positive-strand RNA virus."
Kushner D.B., Lindenbach B.D., Grdzelishvili V.Z., Noueiry A.O., Paul S.M., Ahlquist P.
Proc. Natl. Acad. Sci. U.S.A. 100:15764-15769(2003) [PubMed: 14671320] [Abstract]
Cited for: FUNCTION.
[16]"Spatial organization and dynamics of the association of Rec102 and Rec104 with meiotic chromosomes."
Kee K., Protacio R.U., Arora C., Keeney S.
EMBO J. 23:1815-1824(2004) [PubMed: 15044957] [Abstract]
Cited for: FUNCTION OF THE SKI8-SPO11 COMPLEX.
[17]"Antiviral protein Ski8 is a direct partner of Spo11 in meiotic DNA break formation, independent of its cytoplasmic role in RNA metabolism."
Arora C., Kee K., Maleki S., Keeney S.
Mol. Cell 13:549-559(2004) [PubMed: 14992724] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH SPO11.
[18]"Structure-based assembly of protein complexes in yeast."
Aloy P., Boettcher B., Ceulemans H., Leutwein C., Mellwig C., Fischer S., Gavin A.-C., Bork P., Superti-Furga G., Serrano L., Russell R.B.
Science 303:2026-2029(2004) [PubMed: 15044803] [Abstract]
Cited for: MODELING OF THE SKI COMPLEX 3D-STRUCTURE.
[19]"Both conserved and non-conserved regions of Spo11 are essential for meiotic recombination initiation in yeast."
Nag D.K., Pata J.D., Sironi M., Flood D.R., Hart A.M.
Mol. Genet. Genomics 276:313-321(2006) [PubMed: 16816949] [Abstract]
Cited for: FUNCTION, INTERACTION WITH SPO11.
[20]"The structure of Ski8p, a protein regulating mRNA degradation: Implications for WD protein structure."
Madrona A.Y., Wilson D.K.
Protein Sci. 13:1557-1565(2004) [PubMed: 15152089] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).
[21]"Crystal structure of Ski8p, a WD-repeat protein with dual roles in mRNA metabolism and meiotic recombination."
Cheng Z., Liu Y., Wang C., Parker R., Song H.
Protein Sci. 13:2673-2684(2004) [PubMed: 15340168] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).

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Cross-references

Sequence databases

M96058 mRNA. Translation: AAA35050.1.
Z72736 Genomic DNA. Translation: CAA96930.1.
PIR