Reviewed, UniProtKB/Swiss-Prot Q02956 (KPCZ_MOUSE)

Names and origin

Protein names

Recommended name:
    Protein kinase C zeta type
    EC=2.7.11.13
Alternative name(s):
    nPKC-zeta

Gene names

Name:	Prkcz
Synonyms:	Pkcz

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	592 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Phosphorylates KCNAB2. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Subunit of a quaternary complex that plays a central role in epithelial cell polarization.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation	Phosphatidylinositol 3,4,5-trisphosphate might be a physiological activator. Two specific sites, Thr-410 (activation loop of the kinase domain) and Thr-560 (turn motif), need to be phosphorylated for its full activation.
Subunit structure	Interacts directly with SQSTM1. Forms a ternary complex with SQSTM1 and KCNAB2. Forms another ternary complex with SQSTM1 and GABRR3. Forms a complex with SQSTM1 and MAP2K5 By similarity. Interacts with PARD6A, PARD6B and PARD6G. Part of a complex with PARD3, PARD6A or PARD6B or PARD6G and CDC42 or RAC1. Interacts with CENTA1.
Subcellular location	CytoplasmBy similarity. EndosomeBy similarity. Note= In the retina, localizes in the terminals of the rod bipolar cells. Associated with endosomes By similarity.
Domain	The C1 domain does not bind the diacylglycerol (DAG). The OPR domain mediates mutually exclusive interactions with SQSTM1 and PARD6B By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 OPR domain. Contains 1 phorbol-ester/DAG-type zinc finger. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Cytoplasm Endosome
Domain	Phorbol-ester binding Zinc-finger
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	microtubule cytoskeleton organization and biogenesis Inferred from mutant phenotype. Source: MGI protein amino acid phosphorylation Inferred from direct assay. Source: MGI
Cellular component	apical cortex Inferred from direct assay. Source: MGI tight junction Inferred from direct assay. Source: MGI
Molecular function	protein binding Inferred from physical interaction. Source: IntAct protein kinase activity Inferred from direct assay. Source: MGI
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
C1qbp	O35658	2	EBI-642057,EBI-642072

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 592	592	Protein kinase C zeta type
Regions
	Domain	15 – 98	84	OPR
	Domain	252 – 518	267	Protein kinase
	Domain	519 – 590	72	AGC-kinase C-terminal
	Zinc finger	130 – 180	51	Phorbol-ester/DAG-type
	Nucleotide binding	258 – 266	9	ATP By similarity
	Region	79 – 145	67	Interaction with SQSTM1 By similarity
Sites
	Active site	376	1	Proton acceptor By similarity
	Binding site	281	1	ATP By similarity
Amino acid modifications
	Modified residue	410	1	Phosphothreonine; by PDPK1 By similarity
	Modified residue	560	1	Phosphothreonine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q02956-1 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: F9B4C58D7DD6B181	FASTA	592	67,624
	10 20 30 40 50 60 MPSRTDPKMD RSGGRVRLKA HYGGDILITS VDAMTTFKDL CEEVRDMCGL HQQHPLTLKW 70 80 90 100 110 120 VDSEGDPCTV SSQMELEEAF RLVCQGRDEV LIIHVFPSIP EQPGMPCPGE DKSIYRRGAR 130 140 150 160 170 180 RWRKLYRANG HLFQAKRFNR GAYCGQCSER IWGLSRQGYR CINCKLLVHK RCHVLVPLTC 190 200 210 220 230 240 RRHMDSVMPS QEPPVDGKND GVDLPSEETD GIAYISSSRK HDNIKDDSED LKPVIDGVDG 250 260 270 280 290 300 IKISQGLGLQ DFDLIRVIGR GSYAKVLLVR LKKNDQIYAM KVVKKELVHD DEDIDWVQTE 310 320 330 340 350 360 KHVFEQASSN PFLVGLHSCF QTTSRLFLVI EYVNGGDLMF HMQRQRKLPE EHARFYAAEI 370 380 390 400 410 420 CIALNFLHER GIIYRDLKLD NVLLDADGHI KLTDYGMCKE GLGPGDTTST FCGTPNYIAP 430 440 450 460 470 480 EILRGEEYGF SVDWWALGVL MFEMMAGRSP FDIITDNPDM NTEDYLFQVI LEKPIRIPRF 490 500 510 520 530 540 LSVKASHVLK GFLNKDPKER LGCRPQTGFS DIKSHAFFRS IDWDLLEKKQ TLPPFQPQIT 550 560 570 580 590 DDYGLDNFDT QFTSEPVQLT PDDEDVIKRI DQSEFEGFEY INPLLLSAEE SV

References

[1]	"The cDNA sequence, expression pattern and protein characteristics of mouse protein kinase C-zeta." Goodnight J., Kazanietz M.G., Blumberg P.M., Mushinski F.J., Mischak H. Gene 122:305-311(1992) [PubMed: 1487145] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The cell-polarity protein Par6 links Par3 and atypical protein kinase C to Cdc42." Joberty G., Petersen C., Gao L., Macara I.G. Nat. Cell Biol. 2:531-539(2000) [PubMed: 10934474] [Abstract] Cited for: INTERACTION WITH PARD6B. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M94632 mRNA. Translation: AAA39983.1.
PIR	JC1480.
RefSeq	NP_032886.2.
UniGene	Mm.28561
3D structure databases
HSSP	HSSP built from PDB template 1GZK based on UniProtKB P31751.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q02956.
PTM databases
PhosphoSite	Q02956.
Genome annotation databases
Ensembl	ENSMUSG00000029053. Mus musculus. [Contig view]
GeneID	18762.
Organism-specific databases
MGI	MGI:97602. Prkcz.
Phylogenomic databases
HOGENOM	Q02956.
HOVERGEN	Q02956.
Gene expression databases
ArrayExpress	Q02956.
CleanEx	MM_PRKCZ.
GermOnline	ENSMUSG00000029053. Mus musculus.
Family and domain databases
InterPro	IPR002219. DAG_PE_bd. IPR000270. OPR_PB1. IPR015745. PKC. IPR012233. PKC_zeta. IPR000961. Pkinase_C. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_bd_CS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
PANTHER	PTHR22985:SF86. PKC. 1 hit.
Pfam	PF00130. C1_1. 1 hit. PF00564. PB1. 1 hit. PF00069. Pkinase. 1 hit. PF00433. Pkinase_C. 1 hit. [Graphical view]
PIRSF	PIRSF000554. PKC_zeta. 1 hit.
PRINTS	PR00008. DAGPEDOMAIN.
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00109. C1. 1 hit. SM00666. PB1. 1 hit. SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS51285. AGC_KINASE_CTER. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. PS00479. ZF_DAG_PE_1. 1 hit. PS50081. ZF_DAG_PE_2. 1 hit. [Graphical view]
BLOCKS	Search...
Other Resources
SOURCE	Search...
ProtoNet	Search...

Entry information

Entry name

KPCZ_MOUSE

Accession

Primary (citable) accession number: Q02956

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	July 22, 2008
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)