Reviewed, UniProtKB/Swiss-Prot Q03168 (ASPP_AEDAE)

Names and origin

Protein names

Recommended name:
    Lysosomal aspartic protease
    EC=3.4.23.-

Gene names

ORF Names:

AAEL006169

Organism

Aedes aegypti (Yellowfever mosquito)

Taxonomic identifier

7159 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Nematocera › Culicoidea › Culicidae › Culicinae › Culicini › Aedes › Stegomyia

Protein attributes

Sequence length	387 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May degrade organelles involved in the biosynthesis and secretion of vitellogenin.
Subunit structure	Homodimer.
Subcellular location	Lysosome.
Sequence similarities	Belongs to the peptidase A1 family.

Ontologies

Keywords
Cellular component	Lysosome
Domain	Signal
Molecular function	Aspartyl protease Hydrolase Protease
PTM	Glycoprotein Zymogen
Technical term	Direct protein sequencing
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Signal peptide	1 – 18	18
	Propeptide	19 – 53	35	Removed in mature form
	Chain	54 – 387	334	Lysosomal aspartic protease
Sites
	Active site	86	1	By similarity
	Active site	272	1	By similarity
Amino acid modifications
	Glycosylation	123	1	N-linked (GlcNAc...) Potential
	Disulfide bond	99 ↔ 106		By similarity
	Disulfide bond	263 ↔ 267		By similarity
	Disulfide bond	306 ↔ 343		By similarity
Experimental info
	Sequence conflict	16	1	A → S in AAA29350. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q03168-1 [UniParc]. Last modified July 22, 2008. Version 2. Checksum: B9AD73E444167EEB	FASTA	387	41,790
	10 20 30 40 50 60 MLIKSIIALV CLAVLAQADF VRVQLHKTES ARQHFRNVDT EIKQLRLKYN AVSGPVPEPL 70 80 90 100 110 120 SNYLDAQYYG AITIGTPPQS FKVVFDTGSS NLWVPSKECS FTNIACLMHN KYNAKKSSTF 130 140 150 160 170 180 EKNGTAFHIQ YGSGSLSGYL STDTVGLGGV SVTKQTFAEA INEPGLVFVA AKFDGILGLG 190 200 210 220 230 240 YSSISVDGVV PVFYNMFNQG LIDAPVFSFY LNRDPSAAEG GEIIFGGSDS NKYTGDFTYL 250 260 270 280 290 300 SVDRKAYWQF KMDSVKVGDT EFCNNGCEAI ADTGTSLIAG PVSEVTAINK AIGGTPIMNG 310 320 330 340 350 360 EYMVDCSLIP KLPKISFVLG GKSFDLEGAD YVLRVAQMGK TICLSGFMGI DIPPPNGPLW 370 380 ILGDVFIGKY YTEFDMGNDR VGFATAV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of cDNA for mosquito lysosomal aspartic protease. Sequence analysis of an insect lysosomal enzyme similar to cathepsins D and E." Cho W.L., Raikhel A.S. J. Biol. Chem. 267:21823-21829(1992) [PubMed: 1400492] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 54-71.
[2]	"Genome sequence of Aedes aegypti, a major arbovirus vector." Nene V., Wortman J.R., Lawson D., Haas B.J., Kodira C.D., Tu Z.J., Loftus B.J., Xi Z., Megy K., Grabherr M., Ren Q., Zdobnov E.M., Lobo N.F., Campbell K.S., Brown S.E., Bonaldo M.F., Zhu J., Sinkins S.P. , Hogenkamp D.G., Amedeo P., Arensburger P., Atkinson P.W., Bidwell S.L., Biedler J., Birney E., Bruggner R.V., Costas J., Coy M.R., Crabtree J., Crawford M., DeBruyn B., DeCaprio D., Eiglmeier K., Eisenstadt E., El-Dorry H., Gelbart W.M., Gomes S.L., Hammond M., Hannick L.I., Hogan J.R., Holmes M.H., Jaffe D., Johnston S.J., Kennedy R.C., Koo H., Kravitz S., Kriventseva E.V., Kulp D., Labutti K., Lee E., Li S., Lovin D.D., Mao C., Mauceli E., Menck C.F., Miller J.R., Montgomery P., Mori A., Nascimento A.L., Naveira H.F., Nusbaum C., O'Leary S.B., Orvis J., Pertea M., Quesneville H., Reidenbach K.R., Rogers Y.-H.C., Roth C.W., Schneider J.R., Schatz M., Shumway M., Stanke M., Stinson E.O., Tubio J.M.C., Vanzee J.P., Verjovski-Almeida S., Werner D., White O.R., Wyder S., Zeng Q., Zhao Q., Zhao Y., Hill C.A., Raikhel A.S., Soares M.B., Knudson D.L., Lee N.H., Galagan J., Salzberg S.L., Paulsen I.T., Dimopoulos G., Collins F.H., Bruce B., Fraser-Liggett C.M., Severson D.W. Science 316:1718-1723(2007) [PubMed: 17510324] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Liverpool.
[3]	"Purification and characterization of a lysosomal aspartic protease with cathepsin D activity from the mosquito." Cho W.-L., Dhadialla T.S., Raikhel A.S. Insect Biochem. 21:165-176(1991) Cited for: FUNCTION, SUBCELLULAR LOCATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M95187 mRNA. Translation: AAA29350.1. CH477377 Genomic DNA. Translation: EAT42285.1.
PIR	A45117.
RefSeq	XP_001657556.1.
UniGene	Aae.18587
3D structure databases
HSSP	HSSP built from PDB template 2REN based on UniProtKB P00797.
ModBase	Search...
Protein family/group databases
MEROPS	A01.009.
2-D gel databases
Siena-2DPAGE	Q03168.
Genome annotation databases
Ensembl	AAEL006169. Aedes aegypti. [Contig view]
GeneID	5567565.
VectorBase	AAEL006169. Aedes aegypti.
Family and domain databases
InterPro	IPR001969. Pept_Asp_AS. IPR009007. Pept_Aspartc_cat. IPR001461. Peptidase_A1. IPR012848. Propep_A1. [Graphical view]
Gene3D	G3DSA:2.40.70.10. Pept_Aspartc_cat. 2 hits.
PANTHER	PTHR13683. Peptidase_A1. 1 hit.
Pfam	PF07966. A1_Propeptide. 1 hit. PF00026. Asp. 1 hit. [Graphical view]
PRINTS	PR00792. PEPSIN.
PROSITE	PS00141. ASP_PROTEASE. 2 hits. [Graphical view]
ProDom	Q03168. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

ASPP_AEDAE

Accession

Primary (citable) accession number: Q03168
Secondary accession number(s): Q177E0

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	July 22, 2008
Last modified:	September 2, 2008
This is version 50 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)