Reviewed, UniProtKB/Swiss-Prot Q03210 (NGL3_YEAST)

Names and origin

Protein names

Recommended name:
    Probable RNA exonuclease NGL3
    EC=3.1.-.-

Gene names

Name:	NGL3
Ordered Locus Names:	YML118W
ORF Names:	YM7056.08, YM8339.01

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	505 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Miscellaneous	Present with 414 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the CCR4/nocturin family.

Ontologies

Keywords
Molecular function	Exonuclease Hydrolase Nuclease
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PRP40	P33203	1	EBI-27960,EBI-701

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 505	505	Probable RNA exonuclease NGL3
Amino acid modifications
	Modified residue	62	1	Phosphoserine

Sequences

	Sequence		Length	Mass (Da)
	Q03210-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 2B6D6389A93B7D72	FASTA	505	57,742
	10 20 30 40 50 60 MDSQVEGKIS PSQKESSSTS GLVSPSEDGP AHQKIHRDQL SVDQIKKIRE ERAQKRQVRR 70 80 90 100 110 120 NSLISQGKDP DFPTPDLQFI ERPFLPINHD NSKGLTPATI QVTQDSLDVK IMTYNTLAQT 130 140 150 160 170 180 LIRRDFFPES GPALKWHKRS KVLVHELKKY RPDVVSLQEV DYNELNFWQE NFHKLGFDVI 190 200 210 220 230 240 FKRHEGKTHG LLVAWNNKKF QLDNDWMLDY DNILAGNVIS ARTRTKNIAL IISLYFKGIT 250 260 270 280 290 300 DSSSRGIIVA NTHLFWHPFG VFERLRQSYL VLQKIQEIKA CSKYNGWHSL LMGDFNTEPE 310 320 330 340 350 360 EPPYLAITKR PLILKGPIRA MVECSLAYRY SKKRNGEESD QDDEECDEKS RGEGHSDQPQ 370 380 390 400 410 420 NPKPESFTAT KEEKALVNQL VALHNSLHVK GVSLYGIGYG KVHPENANGS HGEPGLSNWA 430 440 450 460 470 480 NTWCGLLDYI FYIEGDHNQD TRQKEPLNAF EGNNNVKIIG YLRMPCAQEM PKHSQPFEGE 490 500 YASDHISLMC QIRLFFGGEK VHSLK

References

[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[3]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-62, MASS SPECTROMETRY.
[4]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-62, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z49218 Genomic DNA. Translation: CAA89162.1. Z49210 Genomic DNA. Translation: CAA89100.1.
PIR	S54066.
RefSeq	NP_013588.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q03210.
Genome annotation databases
Ensembl	YML118W. Saccharomyces cerevisiae. [Contig view]
GeneID	854921.
GenomeReviews	Gene locus YML118W in contig Z71257_GR.
KEGG	sce:YML118W.
NMPDR	fig|4932.3.peg.4624.
Organism-specific databases
CYGD	YML118w.
SGD	S000004587. NGL3.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	Q03210.
Gene expression databases
GermOnline	YML118W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR005135. Endo/exonuclease/phosphatase. [Graphical view]
Pfam	PF03372. Exo_endo_phos. 1 hit. [Graphical view]
BLOCKS	Search...
Other Resources
LinkHub	Q03210.
ProtoNet	Search...

Entry information

Entry name

NGL3_YEAST

Accession

Primary (citable) accession number: Q03210
Secondary accession number(s): Q03733

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	September 2, 2008
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)