Reviewed, UniProtKB/Swiss-Prot Q03835 (GLRX3_YEAST)

Names and origin

Protein names

Recommended name:
    Monothiol glutaredoxin-3

Gene names

Name:	GRX3
Ordered Locus Names:	YDR098C
ORF Names:	YD8557.05C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	285 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Miscellaneous	Present with 11000 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the glutaredoxin family. Monothiol subfamily. Contains 1 glutaredoxin domain. Contains 1 thioredoxin domain.

Ontologies

Keywords
Biological process	Electron transport Transport
Domain	Redox-active center
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular iron ion homeostasis Inferred from genetic interaction. Source: SGD response to oxidative stress Inferred from mutant phenotype. Source: SGD
Cellular component	cytosol Inferred from direct assay. Source: SGD nucleus Inferred from direct assay. Source: SGD
Molecular function	disulfide oxidoreductase activity Inferred from genetic interaction. Source: SGD protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	P53082	1	EBI-22178,EBI-24159
AFT1	P22149	3	EBI-22178,EBI-2332

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 285	285	Monothiol glutaredoxin-3		PRO_0000102249
Regions
	Domain	30 – 145	116	Thioredoxin
	Domain	186 – 285	100	Glutaredoxin
Sites
	Active site	211	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q03835-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 5C97FFC38192E904	FASTA	285	32,481
	10 20 30 40 50 60 MCSFQVPSAF SFNYTSYCYK RHQARYYTAA KLFQEMPVIE INDQEQFTYL TTTAAGDKLI 70 80 90 100 110 120 VLYFHTSWAE PCKALKQVFE AISNEPSNSN VSFLSIDADE NSEISELFEI SAVPYFIIIH 130 140 150 160 170 180 KGTILKELSG ADPKEYVSLL EDCKNSVNSG SSQTHTMENA NVNEGSHNDE DDDDEEEEEE 190 200 210 220 230 240 TEEQINARLT KLVNAAPVML FMKGSPSEPK CGFSRQLVGI LREHQVRFGF FDILRDESVR 250 260 270 280 QNLKKFSEWP TFPQLYINGE FQGGLDIIKE SLEEDPDFLQ HALQS

References

[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed: 9169867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z47746 Genomic DNA. Translation: CAA87672.1.
PIR	S51247.
RefSeq	NP_010383.1.
3D structure databases
HSSP	HSSP built from PDB template 1TRV based on UniProtKB P10599.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:1350N.
IntAct	Q03835.
Proteomic databases
PeptideAtlas	Q03835.
Genome annotation databases
Ensembl	YDR098C. Saccharomyces cerevisiae. [Contig view]
GeneID	851672.
GenomeReviews	Gene locus YDR098C in contig Z71256_GR.
KEGG	sce:YDR098C.
NMPDR	fig|4932.3.peg.1129.
Organism-specific databases
CYGD	YDR098c.
SGD	S000002505. GRX3.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	Q03835.
Gene expression databases
GermOnline	YDR098C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR002109. Glutaredoxin. IPR004480. Glutredox-rel. IPR006662. Thioredoxin-like. IPR013766. Thioredoxin_dom. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 2 hits.
PANTHER	PTHR10293. Glutredox-rel. 1 hit.
Pfam	PF00462. Glutaredoxin. 1 hit. PF00085. Thioredoxin. 1 hit. [Graphical view]
PRINTS	PR00421. THIOREDOXIN.
TIGRFAMs	TIGR00365. Glutredox-rel. 1 hit.
PROSITE	PS51354. GLUTAREDOXIN_2. 1 hit. PS51352. THIOREDOXIN_2. 1 hit. [Graphical view]
BLOCKS	Search...
ProtoNet	Search...
Other Resources
LinkHub	Q03835.
NextBio	969295.

Entry information

Entry name

GLRX3_YEAST

Accession

Primary (citable) accession number: Q03835

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	November 4, 2008
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)