Reviewed, UniProtKB/Swiss-Prot Q04199 (CAC2_YEAST)

Names and origin

Protein names

Recommended name:
    Chromatin assembly factor 1 subunit p60
Alternative name(s):
    CAF-1 60 kDa subunit

Gene names

Name:	CAC2
Ordered Locus Names:	YML102W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	468 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Acts as a component of chromatin assembly factor 1 (CAF-1), which assembles histone octamers onto replicating DNA in vitro. It performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. p50 and p60 form complexes with newly synthesized histones H3 and acetylated H4 in cell extracts By similarity.
Subunit structure	Component of chromatin assembly factor 1 (CAF-1), which is composed of MSI1/p50, CAC2/p60 and CAC1/p90.
Subcellular location	Nucleus.
Miscellaneous	Present with 2130 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the WD repeat HIR1 family. Contains 5 WD repeats.

Ontologies

Keywords
Cellular component	Nucleus
Domain	Repeat WD repeat
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	DNA repair Ref.2 Inferred from mutant phenotype. Source: SGD chromatin silencing Inferred from mutant phenotype. Source: SGD nucleosome assembly Ref.2 Inferred from mutant phenotype. Source: SGD
Cellular component	CAF-1 complex Ref.2 Inferred from direct assay. Source: SGD chromosome, centromeric region Inferred from direct assay. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct transcription regulator activity Inferred from mutant phenotype. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CAC1	Q12495	1	EBI-3920,EBI-3913
MSI1	P13712	1	EBI-3920,EBI-11391

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 468	468	Chromatin assembly factor 1 subunit p60
Regions
	Repeat	11 – 52	42	WD 1
	Repeat	69 – 108	40	WD 2
	Repeat	143 – 182	40	WD 3
	Repeat	185 – 224	40	WD 4
	Repeat	371 – 413	43	WD 5

Sequences

	Sequence		Length	Mass (Da)
	Q04199-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: F24853C4AA4D97C2	FASTA	468	51,252
	10 20 30 40 50 60 MEASHLQIYW HDSQPVYSLT FQKNSANDKL FTAGGDNKVR IWKLNRDENG QNGGVRKIES 70 80 90 100 110 120 LDFLGSLTHH EQAINVIRFN SKGDVLASAG DDGQVLLWKQ EDPNTQQESV VRPFGMDAET 130 140 150 160 170 180 SEADENKEKW VVWKRLRGGS GATAAAEIYD LAWSPDNRNI VVACMDNSIR LFDVGAGMLV 190 200 210 220 230 240 CGQSDHGHYV QGVAWDPLNQ FILSQSADRS LHVYGVILSS AGVVTGLKLR SKIAKAELPC 250 260 270 280 290 300 PGDVLRTNYL FHNETLPSFF RRCSISPCGG LVVIPSGVYK VAGDEVANCV YVYTRSGILN 310 320 330 340 350 360 SAGGVKNRPA IRIPSLKKPA LMAAFSPVFY ETCQKSVLKL PYKLVFAIAT TNEVLVYDTD 370 380 390 400 410 420 VLEPLCVVGN IHYSPITDLA WSEDGSTLLI SSTDGFCSYV SIDTETQFGS RIEPPAMHAE 430 440 450 460 PLDTDESAVA AKNQREAGGI VNMLPVKKIP CNSSDSKKRR IHPTPVDL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Ultraviolet radiation sensitivity and reduction of telomeric silencing in Saccharomyces cerevisiae cells lacking chromatin assembly factor-I." Kaufman P.D., Kobayashi R., Stillman B. Genes Dev. 11:345-357(1997) [PubMed: 9030687] [Abstract] Cited for: PROTEIN SEQUENCE OF 58-83 AND 459-468, CHARACTERIZATION.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X80835 Genomic DNA. Translation: CAA56795.1.
PIR	S47447.
RefSeq	NP_013605.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:811N.
IntAct	Q04199.
Proteomic databases
PeptideAtlas	Q04199.
Genome annotation databases
Ensembl	YML102W. Saccharomyces cerevisiae. [Contig view]
GeneID	854869.
GenomeReviews	Gene locus YML102W in contig Z71257_GR.
KEGG	sce:YML102W.
NMPDR	fig|4932.3.peg.4641.
Organism-specific databases
CYGD	YML102w.
SGD	S000004570. CAC2.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	Q04199.
Gene expression databases
ArrayExpress	Q04199.
GermOnline	YML102W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR015943. WD40/YVTN_repeat-like. IPR001680. WD40_repeat. [Graphical view]
Gene3D	G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit.
Pfam	PF00400. WD40. 5 hits. [Graphical view]
PRINTS	PR00320. GPROTEINBRPT.
ProDom	PD000018. WD40. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00320. WD40. 5 hits. [Graphical view]
PROSITE	PS00678. WD_REPEATS_1. False negative. PS50082. WD_REPEATS_2. 2 hits. PS50294. WD_REPEATS_REGION. 1 hit. [Graphical view]
BLOCKS	Search...
Other Resources
LinkHub	Q04199.
ProtoNet	Search...

Entry information

Entry name

CAC2_YEAST

Accession

Primary (citable) accession number: Q04199

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	July 22, 2008
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)