Reviewed, UniProtKB/Swiss-Prot Q04412 (AGE1_YEAST)

Names and origin

Protein names

Recommended name:
    ARF GAP effector protein 1
Alternative name(s):
    ADP-ribosylation factor GTPase-activating protein effector protein 1
    Suppressor of ARF1 thermosensitive mutant protein 1

Gene names

Name:	AGE1
Synonyms:	SAT1
Ordered Locus Names:	YDR524C
ORF Names:	D9719.28

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	482 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	GTPase-activating protein (GAP) for the ADP ribosylation factors ARF1 and ARF2. May be involved in the endocytic pathway.
Subcellular location	Cytoplasm.
Miscellaneous	Present with 396 molecules/cell in log phase SD medium.
Sequence similarities	Contains 1 Arf-GAP domain.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cytoplasm
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	GTPase activation
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ER to Golgi vesicle-mediated transport Ref.2 Inferred from genetic interaction. Source: SGD intra-Golgi vesicle-mediated transport Ref.2 Inferred from genetic interaction. Source: SGD
Cellular component	cytoplasm Ref.3 Inferred from direct assay. Source: SGD
Molecular function	ARF GTPase activator activity Ref.2 Ref.5 Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 482	482	ARF GAP effector protein 1		PRO_0000074222
Regions
	Domain	170 – 297	128	Arf-GAP
	Zinc finger	186 – 210	25	C4-type

Sequences

	Sequence		Length	Mass (Da)
	Q04412-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 3F7682F91EA21EDD	FASTA	482	54,494
	10 20 30 40 50 60 MDFYTTDINK NVVPLFSKGT VARTASKAQY PSWCNNALKL TNILLKSLRC KFQTNRCEDD 70 80 90 100 110 120 RGFEVYCVIL KSIALLMAAK ESLILLQIPP SLPSGFPFRS PQLSFTYLST RLSGSQHKST 130 140 150 160 170 180 HSHHINHQTH PIHSSSSNSN SNNRIPTKTD SSKQHTQHFS FANAGASNRD ELLSIVRKID 190 200 210 220 230 240 KSNLKCCDCG STATVEWVSI NLLCILCIKC SGVHRSLGSH ISKIRSLTLD NFTSLELMHL 250 260 270 280 290 300 LQNNVSNSNV NAIYESNLRN FPVKKITANS DDSERSKFII DKYQFKKFVI DSNQGREASL 310 320 330 340 350 360 KSLIKAIHLD SVFMMQRAIA QSKYSLRELT ASEKEQNDLN HSSIFQYSLK HYEIVDGTPI 370 380 390 400 410 420 FFITEFLLCN GIHIDNLPKI TTNWSPKVLE YWETKLEMYG TFQAVNTSRP RSGPHLNMHS 430 440 450 460 470 480 NVDSASSYNK KHDLRVNIPE RSASASKRWS LSSIPKSSQN LMSPTNLLTM HKSLKLAKKD KK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed: 9169867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"A family of Arf effectors defined as suppressors of the loss of Arf function in the yeast Saccharomyces cerevisiae." Zhang C.-J., Cavenagh M.M., Kahn R.A. J. Biol. Chem. 273:19792-19796(1998) [PubMed: 9677411] [Abstract] Cited for: FUNCTION.
[3]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Four ARF GAPs in Saccharomyces cerevisiae have both overlapping and distinct functions." Zhang C.-J., Bowzard J.B., Anido A., Kahn R.A. Yeast 20:315-330(2003) [PubMed: 12627398] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U33057 Genomic DNA. Translation: AAB64964.1.
PIR	S69580.
RefSeq	NP_010812.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:5273N.
IntAct	Q04412.
Proteomic databases
PeptideAtlas	Q04412.
Genome annotation databases
Ensembl	YDR524C. Saccharomyces cerevisiae. [Contig view]
GeneID	852136.
GenomeReviews	Gene locus YDR524C in contig Z71256_GR.
KEGG	sce:YDR524C.
NMPDR	fig|4932.3.peg.1586.
Organism-specific databases
CYGD	YDR524c.
SGD	S000002932. AGE1.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	Q04412.
Gene expression databases
GermOnline	YDR524C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001164. ArfGAP. [Graphical view]
Pfam	PF01412. ArfGap. 1 hit. [Graphical view]
PRINTS	PR00405. REVINTRACTNG.
SMART	SM00105. ArfGap. 1 hit. [Graphical view]
PROSITE	PS50115. ARFGAP. 1 hit. [Graphical view]
BLOCKS	Search...
ProtoNet	Search...
Other Resources
LinkHub	Q04412.
NextBio	970543.

Entry information

Entry name

AGE1_YEAST

Accession

Primary (citable) accession number: Q04412

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2005
Last sequence update:	November 1, 1996
Last modified:	November 4, 2008
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)