Endo-N-acetylneuraminidase - Enterobacteria phage K1F

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Reviewed, UniProtKB/Swiss-Prot Q04830 (ENAN_BPK1F)

Last modified November 4, 2008. Version 58. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein names	Recommended name: Endo-N-acetylneuraminidase Short name=Endo-N EC=3.2.1.129 Alternative name(s): Endosialidase G102
Organism	Enterobacteria phage K1F (Bacteriophage K1F)
Taxonomic identifier	344021 [NCBI]
Taxonomic lineage	Viruses › dsDNA viruses, no RNA stage › Caudovirales › Podoviridae › Autographivirinae › T7-like viruses
Virus host	Escherichia coli [TaxID: 562]

Protein attributes

Sequence length	920 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Responsible for initial absorption of the phage to the host bacterium. Degradation of the alpha-2,8-linked polysialic acid K1 capsule.
Catalytic activity	Endohydrolysis of (2->8)-alpha-sialosyl linkages in oligo- or poly(sialic) acids.
Subunit structure	Homotrimer.
Sequence similarities	Belongs to the glycosyl hydrolase 58 family. Contains 3 BNR repeats.

Ontologies

Keywords
Domain	Repeat
Molecular function	Glycosidase Hydrolase
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	endo-alpha-sialidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

Removed; by host

Chain

919

Endo-N-acetylneuraminidase

PRO_0000057709

Regions

Repeat

12

BNR 1

Repeat

8

BNR 2

Repeat

12

BNR 3

Compositional bias

19

Gly-rich (hinge)

Secondary structure

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920

Helix Strand Turn

Sequences

Sequence

Length

Mass (Da)

Tools

Q04830-1 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 62B77613F55884B3
Show »

920

102,013

        10         20         30         40         50         60 
MSTITQFPSG NTQYRIEFDY LARTFVVVTL VNSSNPTLNR VLEVGRDYRF LNPTMIEMLV 

        70         80         90        100        110        120 
DQSGFDIVRI HRQTGTDLVV DFRNGSVLTA SDLTTAELQA IHIAEEGRDQ TVDLAKEYAD 

       130        140        150        160        170        180 
AAGSSAGNAK DSEDEARRIA ESIRAAGLIG YMTRRSFEKG YNVTTWSEVL LWEEDGDYYR 

       190        200        210        220        230        240 
WDGTLPKNVP AGSTPETSGG IGLGAWVSVG DAALRSQISN PEGAILYPEL HRARWLDEKD 

       250        260        270        280        290        300 
ARGWGAKGDG VTDDTAALTS ALNDTPVGQK INGNGKTYKV TSLPDISRFI NTRFVYERIP 

       310        320        330        340        350        360 
GQPLYYASEE FVQGELFKIT DTPYYNAWPQ DKAFVYENVI YAPYMGSDRH GVSRLHVSWV 

       370        380        390        400        410        420 
KSGDDGQTWS TPEWLTDLHP DYPTVNYHCM SMGVCRNRLF AMIETRTLAK NALTNCALWD 

       430        440        450        460        470        480 
RPMSRSLHLT GGITKAANQR YATIHVPDHG LFVGDFVNFS NSAVTGVSGD MTVATVIDKD 

       490        500        510        520        530        540 
NFTVLTPNQQ TSDLNNAGKN WHMGTSFHKS PWRKTDLGLI PSVTEVHSFA TIDNNGFAMG 

       550        560        570        580        590        600 
YHQGDVAPRE VGLFYFPDAF NSPSNYVRRQ IPSEYEPDAS EPCIKYYDGV LYLITRGTRG 

       610        620        630        640        650        660 
DRLGSSLHRS RDIGQTWESL RFPHNVHRTT LPFAKVGDDL IMFGSERAEN EWEAGAPDDR 

       670        680        690        700        710        720 
YKASYPRTFY ARLNVNNWNA DDIEWVNITD QIYQGGIVNS GVGVGSVVVK DNYIYYMFGG 

       730        740        750        760        770        780 
EDHFNPWTYG DNSAKDPFKS DGHPSDLYCY KMKIGPDNRV SRDFRYGAVP NRAVPVFFDT 

       790        800        810        820        830        840 
NGVRTVPAPM EFTGDLGLGH VTIRASTSSN IRSEVLMEGE YGFIGKSIPT DNPAGQRIIF 

       850        860        870        880        890        900 
CGGEGTSSTT GAQITLYGAN NTDSRRIVYN GDEHLFQSAD VKPYNDNVTA LGGPSNRFTT 

       910        920 
AYLGSNPIVT SNGGGGKQSR

References

[1]	"Complete nucleotide sequence of the bacteriophage K1F tail gene encoding endo-N-acylneuraminidase (endo-N) and comparison to an endo-N homolog in bacteriophage PK1E." Petter J.G., Vimr E.R. J. Bacteriol. 175:4354-4363(1993) [PubMed: 8331067] [Abstract] Cited for: NUCLEOTIDE SEQUENCE, PROTEIN SEQUENCE OF 2-31 AND 917-920.
+	Additional computationally mapped references.

Cross-references

Sequence databases

M63657 Unassigned DNA. Translation: AAC37340.1.

PIR

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1V0E	X-ray	1.90	A/B/C/D/E/F	246-910	[»]
1V0F	X-ray	2.55	A/B/C/D/E/F	246-910	[»]

SMR

Q04830. Positions 246-910.

ModBase

Family and domain databases

InterPro

IPR002860. BNR.
IPR001724. Glyco_hydro_58.
IPR005604. T7_tail_fibre.
[Graphical view]