Reviewed, UniProtKB/Swiss-Prot Q08638 (BGLA_THEMA)

Names and origin

Protein names

Recommended name:
    Beta-glucosidase A
    EC=3.2.1.21
Alternative name(s):
    Gentiobiase
    Cellobiase
    Beta-D-glucoside glucohydrolase

Gene names

Name:

bglA

Organism

Thermotoga maritima

Taxonomic identifier

2336 [NCBI]

Taxonomic lineage

Bacteria › Thermotogae › Thermotogales › Thermotogaceae › Thermotoga

Protein attributes

Sequence length	446 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal, non-reducing beta-D-glucose residues with release of beta-D-glucose.
Pathway	Glycan metabolism; cellulose degradation.
Sequence similarities	Belongs to the glycosyl hydrolase 1 family.
Caution	As the DNA coding for this protein is not found in the complete genome of T.maritima. It could have originated from another bacterial species.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Molecular function	Glycosidase Hydrolase
Technical term	3D-structure
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Molecule processing

Chain

1 – 446

446

Beta-glucosidase A

Sites

Active site

166

1

Proton donor Potential

Active site

351

1

Nucleophile By similarity

Secondary structure

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446

Helix Strand Turn

Details...

Beta strand

11 – 15

5

Helix

18 – 21

4

Helix

27 – 29

3

Helix

34 – 39

6

Helix

46 – 48

3

Beta strand

51 – 53

3

Helix

57 – 70

14

Beta strand

75 – 79

5

Helix

82 – 85

4

Beta strand

89 – 91

3

Helix

95 – 110

16

Beta strand

114 – 122

9

Helix

126 – 129

4

Turn

130 – 132

3

Helix

133 – 135

3

Helix

139 – 154

16

Turn

155 – 157

3

Beta strand

160 – 165

6

Helix

167 – 175

9

Helix

187 – 211

25

Beta strand

216 – 229

14

Helix

238 – 246

9

Helix

249 – 257

9

Helix

262 – 268

7

Helix

269 – 271

3

Helix

276 – 279

4

Helix

280 – 283

4

Beta strand

288 – 302

15

Beta strand

310 – 313

4

Helix

329 – 342

14

Beta strand

347 – 352

6

Helix

369 – 387

19

Beta strand

392 – 398

7

Helix

406 – 411

6

Beta strand

416 – 419

4

Turn

421 – 423

3

Beta strand

426 – 428

3

Helix

430 – 440

11

Sequences

	Sequence		Length	Mass (Da)
	Q08638-1 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 2E5B06E72BF84C01	FASTA	446	51,548
	10 20 30 40 50 60 MNVKKFPEGF LWGVATASYQ IEGSPLADGA GMSIWHTFSH TPGNVKNGDT GDVACDHYNR 70 80 90 100 110 120 WKEDIEIIEK LGVKAYRFSI SWPRILPEGT GRVNQKGLDF YNRIIDTLLE KGITPFVTIY 130 140 150 160 170 180 HWDLPFALQL KGGWANREIA DWFAEYSRVL FENFGDRVKN WITLNEPWVV AIVGHLYGVH 190 200 210 220 230 240 APGMRDIYVA FRAVHNLLRA HARAVKVFRE TVKDGKIGIV FNNGYFEPAS EKEEDIRAVR 250 260 270 280 290 300 FMHQFNNYPL FLNPIYRGDY PELVLEFARE YLPENYKDDM SEIQEKIDFV GLNYYSGHLV 310 320 330 340 350 360 KFDPDAPAKV SFVERDLPKT AMGWEIVPEG IYWILKKVKE EYNPPEVYIT ENGAAFDDVV 370 380 390 400 410 420 SEDGRVHDQN RIDYLKAHIG QAWKAIQEGV PLKGYFVWSL LDNFEWAEGY SKRFGIVYVD 430 440 YSTQKRIVKD SGYWYSNVVK NNGLED

References

[1]

"Comparative amino acid sequence analysis of Thermotoga maritima beta-glucosidase (BglA) deduced from the nucleotide sequence of the gene indicates distant relationship between beta-glucosidases of the BGA family and other families of beta-1,4-glycosyl hydrolases." Liebl W., Gabelsberger J., Schleifer K.H. Mol. Gen. Genet. 242:111-115(1994) [PubMed: 8277941] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X74163 Genomic DNA. Translation: CAA52276.1.

PIR

S34570. S41561.

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1OD0	X-ray	2.11	A/B	2-446	[»]
1OIF	X-ray	2.12	A/B	2-446	[»]
1OIM	X-ray	2.15	A/B	2-446	[»]
1OIN	X-ray	2.15	A/B	2-446	[»]
1UZ1	X-ray	2.00	A/B	2-446	[»]
1W3J	X-ray	2.00	A/B	2-446	[»]
2CBU	X-ray	1.85	A/B	2-446	[»]
2CBV	X-ray	1.95	A/B	2-446	[»]
2CES	X-ray	2.15	A/B	2-446	[»]
2CET	X-ray	1.97	A/B	2-446	[»]
2J75	X-ray	1.85	A/B	2-446	[»]
2J77	X-ray	2.10	A/B	2-446	[»]
2J78	X-ray	1.65	A/B	2-446	[»]
2J79	X-ray	1.94	A/B	2-446	[»]
2J7B	X-ray	1.87	A/B	2-446	[»]
2J7C	X-ray	2.09	A/B	2-446	[»]
2J7D	X-ray	2.24	A/B	2-446	[»]
2J7E	X-ray	2.19	A/B	2-446	[»]
2J7F	X-ray	2.28	A/B	2-446	[»]
2J7G	X-ray	1.91	A/B	2-446	[»]
2J7H	X-ray	1.95	A/B	2-446	[»]
2JAL	X-ray	1.90	A/B	2-446	[»]

ModBase

Search...

Family and domain databases

InterPro

IPR001360. Glyco_hydro_1.
IPR017736. Glyco_hydro_1_beta-glucosidase.
IPR013781. Glyco_hydro_sub_cat.
[Graphical view]

Gene3D

G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.

PANTHER

PTHR10353. Glyco_hydro_1. 1 hit.

Pfam

PF00232. Glyco_hydro_1. 1 hit.
[Graphical view]

PRINTS

PR00131. GLHYDRLASE1.

PROSITE

PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]

ProDom

Q08638.
[Graphical view] [Entries sharing at least one domain]

BLOCKS

Search...

Other Resources

ProtoNet

Search...

Entry information

Entry name

BGLA_THEMA

Accession

Primary (citable) accession number: Q08638

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	September 2, 2008
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)