Reviewed, UniProtKB/Swiss-Prot Q09066 (UREG_HELPY)

Names and origin

Protein names

Recommended name:
    Urease accessory protein ureG

Gene names

Name:	ureG
Ordered Locus Names:	HP_0068

Organism

Helicobacter pylori (Campylobacter pylori) [Complete proteome] [HAMAP]

Taxonomic identifier

210 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	199 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Facilitates the functional incorporation of the urease nickel metallocenter. This process requires GTP hydrolysis, probably effectuated by ureG By similarity.
Subunit structure	Homodimer. UreH, ureF and ureG form a complex that acts as a GTP-hydrolysis-dependent molecular chaperone, activating the urease apoprotein by helping to assemble the nickel containing metallocenter of ureC. The ureE protein probably delivers the nickel By similarity.
Subcellular location	CytoplasmBy similarity.
Miscellaneous	Strains lacking this gene do not express urease. In the K14A mutant normal amounts of apourease and ureG protein are produced.
Sequence similarities	Belongs to the ureG family.

Ontologies

Keywords
Biological process	Nickel insertion Virulence
Cellular component	Cytoplasm
Ligand	GTP-binding Nucleotide-binding
Molecular function	Chaperone
Technical term	Complete proteome
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 199	199	Urease accessory protein ureG
Regions
	Nucleotide binding	8 – 15	8	GTP By similarity
Experimental info
	Mutagenesis	14	1	K → A: No urease activity, no effect on hydrogenase activity
	Sequence conflict	102	1	D → S in AAA25025. Ref.1
	Sequence conflict	167 – 176	10	MRGEKPFIFT → IAAKSPLFLP in AAA25025. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q09066-1 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: A23E448EB6A208B1	FASTA	199	21,955
	10 20 30 40 50 60 MVKIGVCGPV GSGKTALIEA LTRHMSKDYD MAVITNDIYT KEDAEFMCKN SVMPRERIIG 70 80 90 100 110 120 VETGGCPHTA IREDASMNLE AVEEMHGRFP NLELLLIESG GDNLSATFNP ELADFTIFVI 130 140 150 160 170 180 DVAEGDKIPR KGGPGITRSD LLVINKIDLA PYVGADLKVM ERDSKKMRGE KPFIFTNIRA 190 KEGLDDVIAW IKRNALLED

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Expression of Helicobacter pylori urease genes in Escherichia coli grown under nitrogen-limiting conditions." Cussac V., Ferrero R.L., Labigne A. J. Bacteriol. 174:2466-2473(1992) [PubMed: 1313413] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], KNOCKOUT. Strain: 85P.
[2]	"The complete genome sequence of the gastric pathogen Helicobacter pylori." Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J. , Khalak H.G., Glodek A., McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E., Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D., Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S., Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C. Nature 388:539-547(1997) [PubMed: 9252185] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700392 / 26695.
[3]	"Roles of conserved nucleotide-binding domains in accessory proteins, HypB and UreG, in the maturation of nickel-enzymes required for efficient Helicobacter pylori colonization." Mehta N., Benoit S., Maier R.J. Microb. Pathog. 35:229-234(2003) [PubMed: 14521881] [Abstract] Cited for: MUTAGENESIS OF LYS-14. Strain: ATCC 43504 / NCTC 11637 / JCM 7653 / RPH 13487.
[4]	"Bacterial factors that mediate colonization of the stomach and virulence of Helicobacter pylori." Clyne M., Dolan B., Reeves E.P. FEMS Microbiol. Lett. 268:135-143(2007) [PubMed: 17313591] [Abstract] Cited for: REVIEW ON VIRULENCE OF H.PYLORI.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M84338 Genomic DNA. Translation: AAA25025.1. AE000511 Genomic DNA. Translation: AAD07131.1.
PIR	D64528.
RefSeq	NP_206868.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:3131N.
Genome annotation databases
GeneID	899102.
GenomeReviews	Gene locus HP_0068 in contig AE000511_GR.
KEGG	hpy:HP0068.
NMPDR	fig|85962.1.peg.66.
TIGR	HP_0068.
Phylogenomic databases
HOGENOM	Q09066.
Family and domain databases
HAMAP	MF_01389. [Tree]
InterPro	IPR003495. Cbl_biosynth_CobW-like. IPR012202. NiFe_hyd/urase_mat_GTPase. IPR004400. UreG. [Graphical view]
Pfam	PF02492. cobW. 1 hit. [Graphical view]
PIRSF	PIRSF005624. Ni-bind_GTPase. 1 hit.
TIGRFAMs	TIGR00101. ureG. 1 hit.
ProDom	Q09066. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
LinkHub	Q09066.
ProtoNet	Search...

Entry information

Entry name

UREG_HELPY

Accession

Primary (citable) accession number: Q09066

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	November 1, 1997
Last modified:	September 2, 2008
This is version 54 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)