Reviewed, UniProtKB/Swiss-Prot Q09388 (ACM2_CAEEL)

Names and origin

Protein names

Recommended name:
    Probable muscarinic acetylcholine receptor gar-2
Alternative name(s):
    G-protein-linked acetylcholine receptor 2

Gene names

Name:	gar-2
ORF Names:	F47D12.1/F47D12.2

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	627 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Expressed in head region of the larva. In adults, expression is seen in putative sensory neurons, many cells of the ventral cord and in the hermaphrodite-specific neuron (HSN) motor neurons.
Developmental stage	Expressed throughout development from embryonic to adult stage.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane
Molecular function	G-protein coupled receptor Receptor Transducer
Technical term	Complete proteome
Gene Ontology (GO)
None. [Check GOA]

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform a (identifier: Q09388-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform b (identifier: Q09388-2)

Also known as: c;

The sequence of this isoform differs from the canonical sequence as follows:
     520-532: Missing.

Notes: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 627	627	Probable muscarinic acetylcholine receptor gar-2
Regions
	Topological domain	1 – 9	9	Extracellular Potential
	Transmembrane	10 – 30	21	1 Potential
	Topological domain	31 – 41	11	Cytoplasmic Potential
	Transmembrane	42 – 62	21	2 Potential
	Topological domain	63 – 81	19	Extracellular Potential
	Transmembrane	82 – 102	21	3 Potential
	Topological domain	103 – 122	20	Cytoplasmic Potential
	Transmembrane	123 – 143	21	4 Potential
	Topological domain	144 – 172	29	Extracellular Potential
	Transmembrane	173 – 193	21	5 Potential
	Topological domain	194 – 549	356	Cytoplasmic Potential
	Transmembrane	550 – 570	21	6 Potential
	Topological domain	571 – 586	16	Extracellular Potential
	Transmembrane	587 – 609	23	7 Potential
	Topological domain	610 – 627	18	Cytoplasmic Potential
Amino acid modifications
	Disulfide bond	79 ↔ 160		By similarity
Natural variations
	Alternative sequence	520 – 532	13	Missing in isoform b.

Sequences

	Sequence		Length	Mass (Da)
	Isoform a [UniParc]. Last modified June 6, 2002. Version 3. Checksum: CB319E3BA8230AE5	FASTA	627	71,483
	10 20 30 40 50 60 MAVASVLLAL FMLFLSIVTV IGNLAVLLSY YLDKNIRQPT NYFIFSLAIS DLLIGLEGIP 70 80 90 100 110 120 VYTAFYLNNN EWIWGDVLCD LWLSIDYIVC LASIYTVLGI TVDRYYSVKK PATYRNWRTP 130 140 150 160 170 180 GRVVLIIIFI WLVPSILFSV SIFGYGTFTG TGRILKETEC YVQFMTNPYL NMGMYISYYW 190 200 210 220 230 240 TTLFVMLYLY WGIYRAAKKL ALKSDQKTKR LALLTEMRRP EVSVRTSDAG NSSSDSPNDT 250 260 270 280 290 300 SNSSKCFRTA PPTTTVQTTQ TNVGTPPPVF RNHMTLHNNN MDFTKDNEIV RPPTPPDDNT 310 320 330 340 350 360 YSNPNFSMIS EQLTNGFSRQ EPSSVIERES TAPCVSPEPS HASLENEFNE NHHAHFKPEL 370 380 390 400 410 420 SLPFIDADSV SSMVGHDDLR RAMSIRISRS VSMQGTARAT PVIEIVENLE EALKICENLE 430 440 450 460 470 480 ELREDENKNE EEKQKNGLEN GGMNHVIIAN DEQQPSTSKE SEQKEEMTPE NHDPNEVKVP 490 500 510 520 530 540 LIAVSRVESV KSTAGGKVRR LITQMRSHSI RSKRKANKNK SVLSALNFFQ RKKEYKSRSE 550 560 570 580 590 600 NRARKALRTI TFILGSFIIL WTPFYVLATI YGFCETCKAS PSFNTLYTIS YYLCYMNSPL 610 620 NPFCYAMANQ QFKKTLTRIF KGDFRRV
	Isoform b (c) [UniParc]. Checksum: E83DBA510B142DD5		614	69,963
	10 20 30 40 50 60 MAVASVLLAL FMLFLSIVTV IGNLAVLLSY YLDKNIRQPT NYFIFSLAIS DLLIGLEGIP 70 80 90 100 110 120 VYTAFYLNNN EWIWGDVLCD LWLSIDYIVC LASIYTVLGI TVDRYYSVKK PATYRNWRTP 130 140 150 160 170 180 GRVVLIIIFI WLVPSILFSV SIFGYGTFTG TGRILKETEC YVQFMTNPYL NMGMYISYYW 190 200 210 220 230 240 TTLFVMLYLY WGIYRAAKKL ALKSDQKTKR LALLTEMRRP EVSVRTSDAG NSSSDSPNDT 250 260 270 280 290 300 SNSSKCFRTA PPTTTVQTTQ TNVGTPPPVF RNHMTLHNNN MDFTKDNEIV RPPTPPDDNT 310 320 330 340 350 360 YSNPNFSMIS EQLTNGFSRQ EPSSVIERES TAPCVSPEPS HASLENEFNE NHHAHFKPEL 370 380 390 400 410 420 SLPFIDADSV SSMVGHDDLR RAMSIRISRS VSMQGTARAT PVIEIVENLE EALKICENLE 430 440 450 460 470 480 ELREDENKNE EEKQKNGLEN GGMNHVIIAN DEQQPSTSKE SEQKEEMTPE NHDPNEVKVP 490 500 510 520 530 540 LIAVSRVESV KSTAGGKVRR LITQMRSHSI RSKRKANKNK EYKSRSENRA RKALRTITFI 550 560 570 580 590 600 LGSFIILWTP FYVLATIYGF CETCKASPSF NTLYTISYYL CYMNSPLNPF CYAMANQQFK 610 KTLTRIFKGD FRRV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of GAR-2, a novel G protein-linked acetylcholine receptor from Caenorhabditis elegans." Lee Y.-S., Park Y.-S., Nam S., Suh S.J., Lee J., Kaang B.-K., Cho N.J. J. Neurochem. 75:1800-1809(2000) [PubMed: 11032868] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B), TISSUE SPECIFICITY. Strain: Bristol N2.
[2]	"Three functional isoforms of GAR-2, a Caenorhabditis elegans G-protein-linked acetylcholine receptor, are produced by alternative splicing." Suh S.J., Park Y.-S., Lee Y.-S., Cho T.J., Kaang B.-K., Cho N.J. Biochem. Biophys. Res. Commun. 288:1238-1243(2001) [PubMed: 11700045] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING.
[3]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF272738 mRNA. Translation: AAK94896.1. AY053365 mRNA. Translation: AAL15153.1. U22831 Genomic DNA. Translation: AAL65783.1. U22831 Genomic DNA. Translation: AAL65784.1.
RefSeq	NP_001022593.1.
UniGene	Cel.8076
3D structure databases
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
Genome annotation databases
Ensembl	F47D12.1. Caenorhabditis elegans. [Contig view]
GeneID	175893.
KEGG	cel:F47D12.1.
NMPDR	fig|6239.3.peg.10146.
Organism-specific databases
WormBase	WBGene00001518. gar-2.
WormPep	F47D12.1a. CE30134. [WorfDB] F47D12.1b. CE30135. [WorfDB]
Gene expression databases
ArrayExpress	Q09388.
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_supfam. IPR000995. Musac_rcpt. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR00243. MUSCARINICR.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProDom	Q09388. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

ACM2_CAEEL

Accession

Primary (citable) accession number: Q09388
Secondary accession number(s): Q09561, Q95WX7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	June 6, 2002
Last modified:	September 2, 2008
This is version 67 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project