Reviewed, UniProtKB/Swiss-Prot Q0JR25 (IBBR_ORYSJ)

Names and origin

Protein names

Recommended name:
    Bowman-Birk type bran trypsin inhibitor
Alternative name(s):
    Protein RBBI3-3
    RBTI
    OSE727A

Gene names

Name:	RBBI3.3
Ordered Locus Names:	Os01g0124400, LOC_Os01g03360
ORF Names:	P0037C04.19, P0044F08.1

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	254 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Sequence similarities	Belongs to the Bowman-Birk serine protease inhibitor family.
Sequence caution	AAD10378.1 sequence differs from that shown. Reason: Miscellaneous discrepancy. Chimeric cDNA. Its N-terminal part is derived from the gene LOC_Os01g03390, which encoded for an RBBI2.3 protein. BAD52869.1 sequence differs from that shown. Reason: Erroneous gene model prediction. BAF03803.1 sequence differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Domain	Repeat Signal
Molecular function	Protease inhibitor Serine protease inhibitor
Technical term	Direct protein sequencing
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Propeptide	23 – 118	96
	Chain	118 – 254	137	Bowman-Birk type bran trypsin inhibitor
	Propeptide	252 – 254	3
Regions
	Repeat	46 – 120	75
	Repeat	121 – 187	67
	Repeat	188 – 251	64
Sites
	Site	135 – 136	2	Reactive bond for trypsin By similarity
	Site	201 – 202	2	Reactive bond for trypsin By similarity
Amino acid modifications
	Disulfide bond	125 ↔ 185		By similarity
	Disulfide bond	126 ↔ 143		By similarity
	Disulfide bond	152 ↔ 159		By similarity
	Disulfide bond	156 ↔ 172		By similarity
	Disulfide bond	193 ↔ 248		By similarity
	Disulfide bond	194 ↔ 209		By similarity
	Disulfide bond	199 ↔ 207		By similarity
	Disulfide bond	216 ↔ 223		By similarity
	Disulfide bond	220 ↔ 236		By similarity
Experimental info
	Sequence conflict	121	1	R → K in AAB68026 and AAD10378. Ref.5
	Sequence conflict	130	1	K → E in AAB68026 and AAD10378. Ref.5
	Sequence conflict	136 – 137	2	PD → TN in AAB68026 and AAD10378. Ref.5
	Sequence conflict	189	1	P → T in AAB68026 and AAD10378. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q0JR25-1 [UniParc]. Last modified July 10, 2007. Version 2. Checksum: 32630D7DE579C1E8	FASTA	254	27,790
	10 20 30 40 50 60 MSNTTMATST ILLFLLAGLA AAHGDGDTTI RLPSDGAKAS RPRAAKPWDC CDNIEISRLM 70 80 90 100 110 120 IYPPLYRCND EVKQCAAACK ECVEAPGGDF NGGAFVCSDW FSTVDPGPKC TAALDGLSME 130 140 150 160 170 180 RPWKCCDNIK RLPTKPDPPQ WRCNDELEPS QCTAACKSCR EAPGPFPGKL ICEDIYWGAD 190 200 210 220 230 240 PGPFCTPRPW GDCCDKAFCN KMNPPTCRCM DEVKECADAC KDCQRVESSE PPRYVCKDRF 250 TGHPGPVCKP RAEN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, expression and localization pattern of a trypsin inhibitor gene from rice." Masumura T., Fujioka M., Matsui Y., Kumazawa Y., Tashiro M., Morita S., Tanaka K. Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Nipponbare. Tissue: Embryo.
[2]	"The genome sequence and structure of rice chromosome 1." Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T. Nature 420:312-316(2002) [PubMed: 12447438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[4]	"Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana." The rice annotation project (RAP) Genome Res. 17:175-183(2007) [PubMed: 17210932] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[5]	"Nucleotide sequence of a cDNA encoding rice Bowman-Birk proteinase inhibitor." Chen P.-W., Chow S.-H., Chen L.-J. (er) Plant Gene Register PGR97-015 Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 7-254. Strain: cv. Lomello. Tissue: Embryo.
[6]	"The complete amino acid sequence of rice bran trypsin inhibitor." Tashiro M., Hashino K., Shiozaki M., Ibuki F., Maki Z. J. Biochem. 102:297-306(1987) [PubMed: 3667571] [Abstract] Cited for: PROTEIN SEQUENCE OF 119-251. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB098712 mRNA. Translation: BAC78439.1. AP002909 Genomic DNA. Translation: BAB21173.1. AP003233 Genomic DNA. Translation: BAD52869.1. Sequence problems. AP008207 Genomic DNA. Translation: BAF03803.1. Sequence problems. U57640 mRNA. Translation: AAD10378.1. Sequence problems. U76004 mRNA. Translation: AAB68026.1. Different initiation.
PIR	TIRZBR. A27193. T04161.
RefSeq	NP_001041889.1.
UniGene	Os.74608
3D structure databases
HSSP	HSSP built from PDB template 1C2A based on UniProtKB P12940.
ModBase	Search...
Protein family/group databases
MEROPS	I12.007.
Genome annotation databases
GeneID	4326002.
KEGG	osa:4326002.
NMPDR	fig|39947.1.peg.1022.
Organism-specific databases
Gramene	P07084.
Family and domain databases
InterPro	IPR000877. Prot_inh_BBI. [Graphical view]
Gene3D	G3DSA:2.10.69.10. Prot_inh_BBI. 3 hits.
Pfam	PF00228. Bowman-Birk_leg. 6 hits. [Graphical view]
SMART	SM00269. BowB. 3 hits. [Graphical view]
PROSITE	PS00281. BOWMAN_BIRK. 2 hits. [Graphical view]
ProDom	Q0JR25. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

IBBR_ORYSJ

Accession

Primary (citable) accession number: Q0JR25
Secondary accession number(s): P07084 , P93432, Q5ZCA7, Q7XZC9, Q9AWV9, Q9M3W2, Q9SAS2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	July 10, 2007
Last modified:	July 22, 2008
This is version 16 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)