cGMP-dependent protein kinase 2 - Homo sapiens (Human)

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Reviewed, UniProtKB/Swiss-Prot Q13237 (KGP2_HUMAN)

Last modified July 22, 2008. Version 73. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    cGMP-dependent protein kinase 2
      Short name=CGK 2
    EC=2.7.11.12
Alternative name(s):
    Type II cGMP-dependent protein kinase
    cGKII

Gene names

Name:	PRKG2
Synonyms:	PRKGR2

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length	762 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation	Binding of cGMP results in enzyme activation.
Tissue specificity	Highly concentrated in brain, lung and intestinal mucosa.
Sequence similarities	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cGMP subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 2 cyclic nucleotide-binding domains. Contains 1 protein kinase domain.

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Ontologies

Keywords
Coding sequence diversity	Polymorphism
Ligand	ATP-binding Nucleotide-binding cGMP cGMP-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Nitration
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Ref.1 Traceable author statement. Source: ProtInc signal transduction Ref.1 Traceable author statement. Source: ProtInc
Molecular function	protein kinase activity Ref.1 Traceable author statement. Source: ProtInc
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Molecule processing

Chain

1 – 762

762

cGMP-dependent protein kinase 2

Regions

Domain

453 – 711

259

Protein kinase

Domain

712 – 762

AGC-kinase C-terminal

Nucleotide binding

168 – 285

118

cGMP 1

Nucleotide binding

286 – 417

132

cGMP 2

Nucleotide binding

459 – 467

ATP By similarity

Sites

Active site

576

Proton acceptor By similarity

Binding site

482

ATP By similarity

Amino acid modifications

Modified residue

354

Nitrated tyrosine

Natural variations

Natural variant

106

H → R

Natural variant

716

W → R in a colorectal adenocarcinoma sample; somatic mutation.

Experimental info

Sequence conflict

57 – 58

QL → HG in BAA18934. Ref.2

Sequence conflict

154

S → I in CAA76073. Ref.3

Sequence conflict

460

G → A in BAA18934. Ref.2

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Sequences

Sequence

Length

Mass (Da)

Tools

Q13237-1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: FA7BA149906B5996
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FASTA

762

87,432

        10         20         30         40         50         60 
MGNGSVKPKH SKHPDGHSGN LTTDALRNKV TELERELRRK DAEIQEREYH LKELREQLSK 

        70         80         90        100        110        120 
QTVAIAELTE ELQNKCIQLN KLQDVVHMQG GSPLQASPDK VPLEVHRKTS GLVSLHSRRG 

       130        140        150        160        170        180 
AKAGVSAEPT TRTYDLNKPP EFSFEKARVR KDSSEKKLIT DALNKNQFLK RLDPQQIKDM 

       190        200        210        220        230        240 
VECMYGRNYQ QGSYIIKQGE PGNHIFVLAE GRLEVFQGEK LLSSIPMWTT FGELAILYNC 

       250        260        270        280        290        300 
TRTASVKAIT NVKTWALDRE VFQNIMRRTA QARDEQYRNF LRSVSLLKNL PEDKLTKIID 

       310        320        330        340        350        360 
CLEVEYYDKG DYIIREGEEG STFFILAKGK VKVTQSTEGH DQPQLIKTLQ KGEYFGEKAL 

       370        380        390        400        410        420 
ISDDVRSANI IAEENDVACL VIDRETFNQT VGTFEELQKY LEGYVANLNR DDEKRHAKRS 

       430        440        450        460        470        480 
MSNWKLSKAL SLEMIQLKEK VARFSSSSPF QNLEIIATLG VGGFGRVELV KVKNENVAFA 

       490        500        510        520        530        540 
MKCIRKKHIV DTKQQEHVYS EKRILEELCS PFIVKLYRTF KDNKYVYMLL EACLGGELWS 

       550        560        570        580        590        600 
ILRDRGSFDE PTSKFCVACV TEAFDYLHRL GIIYRDLKPE NLILDAEGYL KLVDFGFAKK 

       610        620        630        640        650        660 
IGSGQKTWTF CGTPEYVAPE VILNKGHDFS VDFWSLGILV YELLTGNPPF SGVDQMMTYN 

       670        680        690        700        710        720 
LILKGIEKMD FPRKITRRPE DLIRRLCRQN PTERLGNLKN GINDIKKHRW LNGFNWEGLK 

       730        740        750        760 
ARSLPSPLQR ELKGPIDHSY FDKYPPEKGM PPDELSGWDK DF

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning, cDNA structure, and chromosomal localization of the human type II cGMP-dependent protein kinase." Orstavik S., Solberg R., Taskn K., Nordahl M., Altherr M.R., Hansson V., Jahnsen T., Sandberg M. Biochem. Biophys. Res. Commun. 220:759-765(1996) [PubMed: 8607838] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Expression of the human cGMP-dependent protein kinase II gene is lost upon introduction of SV40 T antigen or immortalization in human cells." Fujii M., Ogata T., Takahashi E., Yamada K., Nakabayashi K., Oishi M., Ayusawa D. FEBS Lett. 375:263-267(1995) [PubMed: 7498513] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Characterization of the gene encoding the human type II cGMP-dependent protein kinase." Witczak O., Orstavik S., Natarajan V., Frengen E., Jahnsen T., Sandberg M. Biochem. Biophys. Res. Commun. 245:113-119(1998) [PubMed: 9535793] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-734.
[4]	"The human pituitary nitroproteome: detection of nitrotyrosyl-proteins with two-dimensional Western blotting, and amino acid sequence determination with mass spectrometry." Zhan X., Desiderio D.M. Biochem. Biophys. Res. Commun. 325:1180-1186(2004) [PubMed: 15555551] [Abstract] Cited for: NITRATION [LARGE SCALE ANALYSIS] AT TYR-354, MASS SPECTROMETRY. Tissue: Pituitary.
[5]	"Patterns of somatic mutation in human cancer genomes." Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. Stratton M.R. Nature 446:153-158(2007) [PubMed: 17344846] [Abstract] Cited for: VARIANTS [LARGE SCALE ANALYSIS] ARG-106 AND ARG-716.

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Cross-references

Sequence databases
	X94612 mRNA. Translation: CAA64318.1. D70899 mRNA. Translation: BAA18934.1. Y16106 Y16123 Genomic DNA. Translation: CAA76073.1.
PIR	S68217.
RefSeq	NP_006250.1.
UniGene	Hs.570833
3D structure databases
HSSP	HSSP built from PDB template 1CTP based on UniProtKB P36887.
ModBase	Search...
PTM databases
PhosphoSite	Q13237.
Genome annotation databases
Ensembl	ENSG00000138669. Homo sapiens. [Contig view]
GeneID	5593.
KEGG	hsa:5593.
Organism-specific databases
H-InvDB	HIX0031503.
HGNC	HGNC:9416. PRKG2.
HPA	HPA007386.
MIM	601591. gene.
PharmGKB	PA33778.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOGENOM	Q13237.
HOVERGEN	Q13237.
Gene expression databases
ArrayExpress	Q13237.
CleanEx	HS_PRKG2.
GermOnline	ENSG00000138669. Homo sapiens.
Family and domain databases
InterPro	IPR016232. cGMP-dependent_protein_kinase. IPR002374. cGMP_kinase_core. IPR000595. cNMP_bd. IPR000961. Pkinase_C. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_bd_CS. IPR014710. RmlC-like_jellyroll. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Gene3D	G3DSA:2.60.120.10. RmlC-like_jellyroll. 2 hits.
Pfam	PF00027. cNMP_binding. 2 hits. PF00069. Pkinase. 1 hit. [Graphical view]
PIRSF	PIRSF000559. cGMP-dep_kinase. 1 hit.
PRINTS	PR00104. CGMPKINASE.
ProDom	PD000001. Prot_kinase. 2 hits. [Graphical view] [Entries sharing at least one domain]
SMART	SM00100. cNMP. 2 hits. SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS51285. AGC_KINASE_CTER. 1 hit. PS00888. CNMP_BINDING_1. 2 hits. PS00889. CNMP_BINDING_2. 2 hits. PS50042. CNMP_BINDING_3. 2 hits. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
BLOCKS	Search...
Other Resources
SOURCE	Search...
ProtoNet	Search...

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Entry information

Entry name

KGP2_HUMAN

Accession

Primary (citable) accession number: Q13237
Secondary accession number(s): O00125, O60916

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1996
Last modified:	July 22, 2008
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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