Reviewed, UniProtKB/Swiss-Prot Q13490 (BIRC2_HUMAN)

Names and origin

Protein names

Recommended name:
    Baculoviral IAP repeat-containing protein 2
Alternative name(s):
    Inhibitor of apoptosis protein 2
      Short name(s)=HIAP-2, HIAP2
    C-IAP1
    TNFR2-TRAF-signaling complex protein 2
    IAP homolog B
    RING finger protein 48

Gene names

Name:	BIRC2
Synonyms:	API1, IAP2, MIHB, RNF48

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	618 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Apoptotic suppressor. The BIR motifs region interacts with TNF receptor associated factors 1 and 2 (TRAF1 and TRAF2) to form an heteromeric complex, which is then recruited to the tumor necrosis factor receptor 2 (TNFR2).
Subunit structure	Interacts with SMAC and with PRSS25; these interactions inhibit apoptotic suppressor activity.
Subcellular location	CytoplasmPotential.
Tissue specificity	Present in many fetal and adult tissues. Mainly expressed in adult skeletal muscle, thymus, testis, ovary, and pancreas, low or absent in brain and peripheral blood leukocytes.
Sequence similarities	Belongs to the IAP family. Contains 3 BIR repeats. Contains 1 CARD domain. Contains 1 RING-type zinc finger.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Cytoplasm
Coding sequence diversity	Polymorphism
Domain	Repeat Zinc-finger
Ligand	Metal-binding Zinc
Technical term	3D-structure
Gene Ontology (GO)
Biological process	cell surface receptor linked signal transduction Ref.1 Traceable author statement. Source: ProtInc positive regulation of I-kappaB kinase/NF-kappaB cascade Inferred from expression pattern. Source: UniProtKB
Cellular component	cytosol Inferred from Experiment. Source: Reactome
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CASP3	P42574	1	EBI-514538,EBI-524064
CASP7	P55210	2	EBI-514538,EBI-523958
CASP9	P55211	1	EBI-514538,EBI-516799
DIABLO	Q9NR28	2	EBI-514538,EBI-517508
MAP3K14	Q99558	1	EBI-514538,EBI-358011
TRAF2	Q12933	3	EBI-514538,EBI-355744

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Molecule processing

Chain

1 – 618

618

Baculoviral IAP repeat-containing protein 2

Regions

Repeat

46 – 113

68

BIR 1

Repeat

184 – 250

67

BIR 2

Repeat

269 – 336

68

BIR 3

Domain

453 – 543

91

CARD

Zinc finger

571 – 606

36

RING-type

Natural variations

Natural variant

453

1

M → V

Natural variant

506

1

A → V

Natural variant

549

1

P → S

Experimental info

Sequence conflict

157

1

S → P in AAC50372. Ref.2

Sequence conflict

308

1

C → G in AAC50372. Ref.2

Sequence conflict

414

1

Q → L in AAC50372. Ref.2

Sequence conflict

514

1

L → W in AAC50372. Ref.2

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

618

Helix Strand Turn

Details...

Beta strand

266 – 268

3

Helix

271 – 274

4

Beta strand

275 – 277

3

Beta strand

279 – 282

4

Helix

287 – 293

7

Turn

301 – 304

4

Beta strand

307 – 311

5

Helix

322 – 325

4

Turn

334 – 343

10

Helix

344 – 351

8

Sequences

	Sequence		Length	Mass (Da)
	Q13490-1 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: C1778D328063586D	FASTA	618	69,900
	10 20 30 40 50 60 MHKTASQRLF PGPSYQNIKS IMEDSTILSD WTNSNKQKMK YDFSCELYRM STYSTFPAGV 70 80 90 100 110 120 PVSERSLARA GFYYTGVNDK VKCFCCGLML DNWKLGDSPI QKHKQLYPSC SFIQNLVSAS 130 140 150 160 170 180 LGSTSKNTSP MRNSFAHSLS PTLEHSSLFS GSYSSLSPNP LNSRAVEDIS SSRTNPYSYA 190 200 210 220 230 240 MSTEEARFLT YHMWPLTFLS PSELARAGFY YIGPGDRVAC FACGGKLSNW EPKDDAMSEH 250 260 270 280 290 300 RRHFPNCPFL ENSLETLRFS ISNLSMQTHA ARMRTFMYWP SSVPVQPEQL ASAGFYYVGR 310 320 330 340 350 360 NDDVKCFCCD GGLRCWESGD DPWVEHAKWF PRCEFLIRMK GQEFVDEIQG RYPHLLEQLL 370 380 390 400 410 420 STSDTTGEEN ADPPIIHFGP GESSSEDAVM MNTPVVKSAL EMGFNRDLVK QTVQSKILTT 430 440 450 460 470 480 GENYKTVNDI VSALLNAEDE KREEEKEKQA EEMASDDLSL IRKNRMALFQ QLTCVLPILD 490 500 510 520 530 540 NLLKANVINK QEHDIIKQKT QIPLQARELI DTILVKGNAA ANIFKNCLKE IDSTLYKNLF 550 560 570 580 590 600 VDKNMKYIPT EDVSGLSLEE QLRRLQEERT CKVCMDKEVS VVFIPCGHLV VCQECAPSLR 610 KCPICRGIIK GTVRTFLS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The TNFR2-TRAF signaling complex contains two novel proteins related to baculoviral inhibitor of apoptosis proteins." Rothe M., Pan M.-G., Henzel W.J., Ayres T.M., Goeddel D.V. Cell 83:1243-1252(1995) [PubMed: 8548810] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Suppression of apoptosis in mammalian cells by NAIP and a related family of IAP genes." Liston P., Roy N., Tamai K., Lefebvre C., Baird S., Cherton-Horvat G., Farahani R., McLean M., Ikeda J., Mackenzie A., Korneluk R.G. Nature 379:349-353(1996) [PubMed: 8552191] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[3]	"Cloning and expression of apoptosis inhibitory protein homologs that function to inhibit apoptosis and/or bind tumor necrosis factor receptor-associated factors." Uren A.G., Pakusch M., Hawkins C.J., Puls K.L., Vaux D.L. Proc. Natl. Acad. Sci. U.S.A. 93:4974-4978(1996) [PubMed: 8643514] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Fetal liver.
[4]	"NIEHS-SNPs, environmental genome project, NIEHS ES15478, Department of Genome Sciences, Seattle, WA (URL: http://egp.gs.washington.edu)." Livingston R.J., Rieder M.J., Shaffer T., Bertucci C., Baier C.N., Rajkumar N., Willa H.T., Daniels M., Downing T.K., Stanaway I.B., Nguyen C.P., Gildersleeve H., Cassidy C.M., Johnson E.J., Swanson J.E., McFarland I., Yool B., Park C., Nickerson D.A. Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS VAL-453; VAL-506 AND SER-549.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis and Uterus.
[6]	"Solution structure of a baculoviral inhibitor of apoptosis (IAP) repeat." Hinds M.G., Norton R.S., Vaux D.L., Day C.L. Nat. Struct. Biol. 6:648-651(1999) [PubMed: 10404221] [Abstract] Cited for: STRUCTURE BY NMR OF 266-363.

Cross-references

Sequence databases

EMBLGenBankDDBJ

L49431 mRNA. Translation: AAC41942.1.
U45879 mRNA. Translation: AAC50372.1.
U37547 mRNA. Translation: AAC50508.1.
DQ068066 Genomic DNA. Translation: AAY46158.1.
BC016174 mRNA. Translation: AAH16174.1.
BC028578 mRNA. Translation: AAH28578.1.

PIR

S68450.

RefSeq

NP_001157.1.

UniGene

Hs.696238

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1QBH	NMR	-	A	266-363	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

Q13490.

Protein family/group databases

MEROPS

I32.002.

Polymorphism databases

NIEHS-SNPs

Search...

Genome annotation databases

Ensembl

ENSG00000110330. Homo sapiens. [Contig view]

GeneID

329.

KEGG

hsa:329.

Organism-specific databases

H-InvDB

HIX0010063.

HGNC

HGNC:590. BIRC2.

HPA

HPA005513.

MIM

601712. gene.

PharmGKB

PA25359.

GenAtlas

Search...

GeneCards

Search...

GeneLynx

Search...

Phylogenomic databases

HOGENOM

Q13490.

HOVERGEN

Q13490.

Gene expression databases

ArrayExpress

Q13490.

CleanEx

HS_BIRC2.

GermOnline

ENSG00000110330. Homo sapiens.

Family and domain databases

InterPro

IPR001315. CARD.
IPR011029. DEATH_like.
IPR001370. Prot_inh_I32_IAP.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]

Gene3D

G3DSA:1.10.533.10. DEATH_like. 1 hit.
G3DSA:3.30.40.10. Znf_RING/FYVE/PHD. 1 hit.

Pfam

PF00653. BIR. 3 hits.
PF00619. CARD. 1 hit.
PF00097. zf-C3HC4. 1 hit.
[Graphical view]

SMART

SM00238. BIR. 3 hits.
SM00114. CARD. 1 hit.
SM00184. RING. 1 hit.
[Graphical view]

PROSITE

PS01282. BIR_REPEAT_1. 3 hits.
PS50143. BIR_REPEAT_2. 3 hits.
PS50209. CARD. 1 hit.
PS00518. ZF_RING_1. False negative.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

ProDom

Q13490.
[Graphical view] [Entries sharing at least one domain]

BLOCKS

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Other Resources

LinkHub

Q13490.

SOURCE

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ProtoNet

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Entry information

Entry name

BIRC2_HUMAN

Accession

Primary (citable) accession number: Q13490
Secondary accession number(s): Q16516, Q4TTG0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	July 22, 2008
This is version 83 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)