Reviewed, UniProtKB/Swiss-Prot Q13508 (NAR3_HUMAN)

Names and origin

Protein names

Recommended name:
    Ecto-ADP-ribosyltransferase 3
    EC=2.4.2.31
Alternative name(s):
    NAD(P)(+)--arginine ADP-ribosyltransferase 3
    Mono(ADP-ribosyl)transferase 3

Gene names

Name:	ART3
Synonyms:	TMART

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	389 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	NAD(+) + protein-L-arginine = nicotinamide + N(omega)-(ADP-D-ribosyl)-protein-L-arginine. NADP(+) + protein-L-arginine = nicotinamide + N(omega)-((2'-phospho-ADP)-D-ribosyl)-protein-L-arginine.
Subcellular location	Cell membrane; Lipid-anchor › GPI-anchor.
Tissue specificity	Testis specific.
Post-translational modification	Contains two disulfide bonds By similarity.
Sequence similarities	Belongs to the Arg-specific ADP-ribosyltransferase family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing
Domain	Repeat Signal
Ligand	NAD NADP
Molecular function	Glycosyltransferase Transferase
PTM	GPI-anchor Glycoprotein Lipoprotein
Gene Ontology (GO)
Biological process	protein amino acid ADP-ribosylation Ref.2 Traceable author statement. Source: ProtInc
Cellular component	integral to plasma membrane Ref.2 Traceable author statement. Source: ProtInc
Molecular function	NAD+ ADP-ribosyltransferase activity Ref.2 Traceable author statement. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Notes: Experimental confirmation may be lacking for some isoforms.

Isoform 3 (identifier: Q13508-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 1 (identifier: Q13508-2)

The sequence of this isoform differs from the canonical sequence as follows:
     324-346: GMKIPEPFPLPEDKSQGNINNPT → A

Isoform 2 (identifier: Q13508-3)

The sequence of this isoform differs from the canonical sequence as follows:
     324-334: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Signal peptide	1 – 26	26	Potential
	Chain	27 – 362	336	Ecto-ADP-ribosyltransferase 3
	Propeptide	363 – 389	27	Removed in mature form By similarity
Regions
	Repeat	283 – 292	10	1
	Repeat	293 – 302	10	2
	Repeat	303 – 312	10	3
	Region	283 – 312	30	3 X 10 AA tandem repeats of [GS]-E-K-N-[QW]-K-L-E-D-H
Sites
	Active site	229	1	By similarity
Amino acid modifications
	Lipidation	362	1	GPI-anchor amidated serine By similarity
	Glycosylation	248	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	324 – 346	23	GMKIP…INNPT → A in isoform 1.
	Alternative sequence	324 – 334	11	Missing in isoform 2.
Experimental info
	Sequence conflict	357	1	S → C in CAA65096. Ref.2
	Sequence conflict	363	1	S → L in AAH08397. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 3 [UniParc]. Last modified August 13, 2002. Version 2. Checksum: EE1311304BB2F41D	FASTA	389	43,923
	10 20 30 40 50 60 MKTGHFEIVT MLLATMILVD IFQVKAEVLD MADNAFDDEY LKCTDRMEIK YVPQLLKEEK 70 80 90 100 110 120 ASHQQLDTVW ENAKAKWAAR KTQIFLPMNF KDNHGIALMA YISEAQEQTP FYHLFSEAVK 130 140 150 160 170 180 MAGQSREDYI YGFQFKAFHF YLTRALQLLR KPCEASSKTV VYRTSQGTSF TFGGLNQARF 190 200 210 220 230 240 GHFTLAYSAK PQAANDQLTV LSIYTCLGVD IENFLDKESE RITLIPLNEV FQVSQEGAGN 250 260 270 280 290 300 NLILQSINKT CSHYECAFLG GLKTENCIEN LEYFQPIYVY NPGEKNQKLE DHSEKNWKLE 310 320 330 340 350 360 DHGEKNQKLE DHGVKILEPT QIPGMKIPEP FPLPEDKSQG NINNPTPGPV PVPGPKSHPS 370 380 ASSGKLLLPQ FGMVIILISV SAINLFVAL
	Isoform 1 [UniParc]. Checksum: 2741C884A7C685A2		367	41,489
	10 20 30 40 50 60 MKTGHFEIVT MLLATMILVD IFQVKAEVLD MADNAFDDEY LKCTDRMEIK YVPQLLKEEK 70 80 90 100 110 120 ASHQQLDTVW ENAKAKWAAR KTQIFLPMNF KDNHGIALMA YISEAQEQTP FYHLFSEAVK 130 140 150 160 170 180 MAGQSREDYI YGFQFKAFHF YLTRALQLLR KPCEASSKTV VYRTSQGTSF TFGGLNQARF 190 200 210 220 230 240 GHFTLAYSAK PQAANDQLTV LSIYTCLGVD IENFLDKESE RITLIPLNEV FQVSQEGAGN 250 260 270 280 290 300 NLILQSINKT CSHYECAFLG GLKTENCIEN LEYFQPIYVY NPGEKNQKLE DHSEKNWKLE 310 320 330 340 350 360 DHGEKNQKLE DHGVKILEPT QIPAPGPVPV PGPKSHPSAS SGKLLLPQFG MVIILISVSA INLFVAL
	Isoform 2 [UniParc]. Checksum: 210DDA8EB3ABEA56		378	42,716
	10 20 30 40 50 60 MKTGHFEIVT MLLATMILVD IFQVKAEVLD MADNAFDDEY LKCTDRMEIK YVPQLLKEEK 70 80 90 100 110 120 ASHQQLDTVW ENAKAKWAAR KTQIFLPMNF KDNHGIALMA YISEAQEQTP FYHLFSEAVK 130 140 150 160 170 180 MAGQSREDYI YGFQFKAFHF YLTRALQLLR KPCEASSKTV VYRTSQGTSF TFGGLNQARF 190 200 210 220 230 240 GHFTLAYSAK PQAANDQLTV LSIYTCLGVD IENFLDKESE RITLIPLNEV FQVSQEGAGN 250 260 270 280 290 300 NLILQSINKT CSHYECAFLG GLKTENCIEN LEYFQPIYVY NPGEKNQKLE DHSEKNWKLE 310 320 330 340 350 360 DHGEKNQKLE DHGVKILEPT QIPEDKSQGN INNPTPGPVP VPGPKSHPSA SSGKLLLPQF 370 GMVIILISVS AINLFVAL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human testis specifically expresses a homologue of the rodent T lymphocytes RT6 mRNA." Levy I., Wu Y.-Q., Roeckel N., Bulle F., Pawlak A., Siegrist S., Mattei M.-G., Guellaen G. FEBS Lett. 382:276-280(1996) [PubMed: 8605984] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Testis.
[2]	"Two novel human members of an emerging mammalian gene family related to mono-ADP-ribosylating bacterial toxins." Koch-Nolte F., Haag F., Braren R., Kuehl M., Hoovers J., Balasubramanian S., Bazan J.F., Thiele H.-G. Genomics 39:370-376(1997) [PubMed: 9119374] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3). Tissue: Skeletal muscle.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U47054 mRNA. Translation: AAB01894.1. X95827 Genomic DNA. Translation: CAA65096.1. BC008397 mRNA. Translation: AAH08397.1. BC017913 mRNA. Translation: AAH17913.1.
PIR	S62906.
RefSeq	NP_001170.2.
UniGene	Hs.24976
3D structure databases
HSSP	HSSP built from PDB template 1GXY based on UniProtKB P20974.
ModBase	Search...
Proteomic databases
PeptideAtlas	Q13508.
Genome annotation databases
Ensembl	ENSG00000156219. Homo sapiens. [Contig view]
GeneID	419.
KEGG	hsa:419.
Organism-specific databases
H-InvDB	HIX0004304.
HGNC	HGNC:725. ART3.
HPA	HPA011268.
MIM	603086. gene.
PharmGKB	PA25016.
GenAtlas	Search...
GeneCards	Search...
GeneLynx	Search...
Phylogenomic databases
HOGENOM	Q13508.
HOVERGEN	Q13508.
Gene expression databases
ArrayExpress	Q13508.
CleanEx	HS_ART3.
GermOnline	ENSG00000156219. Homo sapiens.
Family and domain databases
InterPro	IPR000768. ART. [Graphical view]
PANTHER	PTHR10339. ART. 1 hit.
Pfam	PF01129. ART. 1 hit. [Graphical view]
PRINTS	PR00970. RIBTRNSFRASE.
PROSITE	PS01291. ART. 1 hit. [Graphical view]
ProDom	Q13508. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
LinkHub	Q13508.
SOURCE	Search...
ProtoNet	Search...

Entry information

Entry name

NAR3_HUMAN

Accession

Primary (citable) accession number: Q13508
Secondary accession number(s): Q8WVJ7, Q93069, Q96HL1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	August 13, 2002
Last modified:	July 22, 2008
This is version 74 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)