Reviewed, UniProtKB/Swiss-Prot Q14738 (2A5D_HUMAN)

Names and origin

Protein names

Recommended name:
    Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform
Alternative name(s):
    PP2A, B subunit, B' delta isoform
    PP2A, B subunit, B56 delta isoform
    PP2A, B subunit, PR61 delta isoform
    PP2A, B subunit, R5 delta isoform

Gene names

Name:

PPP2R5D

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	602 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.
Subunit structure	PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules.
Subcellular location	Cytoplasm. Nucleus. Note= Nuclear in interphase, nuclear during mitosis.
Tissue specificity	Isoform Delta-2 is widely expressed. Isoform Delta-1 is highly expressed in brain.
Induction	By retinoic acid; in neuroblastoma cell lines.
Post-translational modification	Phosphorylated upon DNA damage, probably by ATM or ATR.
Sequence similarities	Belongs to the phosphatase 2A regulatory subunit B56 family.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing
Domain	Repeat
PTM	Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	nervous system development Ref.1 Traceable author statement. Source: ProtInc
Cellular component	nucleus Ref.1 Traceable author statement. Source: ProtInc
Molecular function	protein binding Inferred from physical interaction. Source: IntAct protein phosphatase type 2A regulator activity Ref.1 Traceable author statement. Source: ProtInc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CDC25C	P30307	1	EBI-396563,EBI-974439
PPFIA1	Q13136	1	EBI-396563,EBI-745426
PPP2R1B	P30154	1	EBI-396563,EBI-357094

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform Delta-1 (identifier: Q14738-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Delta-2 (identifier: Q14738-2)

The sequence of this isoform differs from the canonical sequence as follows:
     85-116: Missing.

Isoform Delta-3 (identifier: Q14738-3)

The sequence of this isoform differs from the canonical sequence as follows:
     11-116: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 602	602	Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform		PRO_0000071452
Regions
	Repeat	37 – 38	2	1
	Repeat	39 – 40	2	2
	Repeat	41 – 42	2	3
	Repeat	43 – 44	2	4
	Repeat	45 – 46	2	5
	Repeat	47 – 48	2	6; approximate
	Repeat	49 – 50	2	7; approximate
	Repeat	51 – 52	2	8
	Region	37 – 52	16	8 X 2 AA approximate tandem repeats of Q-P
	Motif	523 – 530	8	SH3-binding; class I Potential
	Motif	548 – 565	18	Nuclear localization signal Potential
Amino acid modifications
	Modified residue	553	1	Phosphothreonine
	Modified residue	598	1	Phosphoserine
Natural variations
	Alternative sequence	11 – 116	106	Missing in isoform Delta-3.		VSP_005110
	Alternative sequence	85 – 116	32	Missing in isoform Delta-2.		VSP_005111

Sequences

	Sequence		Length	Mass (Da)
	Isoform Delta-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: F15F71AF4E565387	FASTA	602	69,992
	10 20 30 40 50 60 MPYKLKKEKE PPKVAKCTAK PSSSGKDGGG ENTEEAQPQP QPQPQPQAQS QPPSSNKRPS 70 80 90 100 110 120 NSTPPPTQLS KIKYSGGPQI VKKERRQSSS RFNLSKNREL QKLPALKDSP TQEREELFIQ 130 140 150 160 170 180 KLRQCCVLFD FVSDPLSDLK FKEVKRAGLN EMVEYITHSR DVVTEAIYPE AVTMFSVNLF 190 200 210 220 230 240 RTLPPSSNPT GAEFDPEEDE PTLEAAWPHL QLVYEFFLRF LESPDFQPNI AKKYIDQKFV 250 260 270 280 290 300 LALLDLFDSE DPRERDFLKT ILHRIYGKFL GLRAYIRRQI NHIFYRFIYE TEHHNGIAEL 310 320 330 340 350 360 LEILGSIING FALPLKEEHK MFLIRVLLPL HKVKSLSVYH PQLAYCVVQF LEKESSLTEP 370 380 390 400 410 420 VIVGLLKFWP KTHSPKEVMF LNELEEILDV IEPSEFSKVM EPLFRQLAKC VSSPHFQVAE 430 440 450 460 470 480 RALYYWNNEY IMSLISDNAA RVLPIMFPAL YRNSKSHWNK TIHGLIYNAL KLFMEMNQKL 490 500 510 520 530 540 FDDCTQQYKA EKQKGRFRMK EREEMWQKIE ELARLNPQYP MFRAPPPLPP VYSMETETPT 550 560 570 580 590 600 AEDIQLLKRT VETEAVQMLK DIKKEKVLLR RKSELPQDVY TIKALEAHKR AEEFLTASQE AL
	Isoform Delta-2 [UniParc]. Checksum: 978213A7CD1B21A5		570	66,181
	10 20 30 40 50 60 MPYKLKKEKE PPKVAKCTAK PSSSGKDGGG ENTEEAQPQP QPQPQPQAQS QPPSSNKRPS 70 80 90 100 110 120 NSTPPPTQLS KIKYSGGPQI VKKELFIQKL RQCCVLFDFV SDPLSDLKFK EVKRAGLNEM 130 140 150 160 170 180 VEYITHSRDV VTEAIYPEAV TMFSVNLFRT LPPSSNPTGA EFDPEEDEPT LEAAWPHLQL 190 200 210 220 230 240 VYEFFLRFLE SPDFQPNIAK KYIDQKFVLA LLDLFDSEDP RERDFLKTIL HRIYGKFLGL 250 260 270 280 290 300 RAYIRRQINH IFYRFIYETE HHNGIAELLE ILGSIINGFA LPLKEEHKMF LIRVLLPLHK 310 320 330 340 350 360 VKSLSVYHPQ LAYCVVQFLE KESSLTEPVI VGLLKFWPKT HSPKEVMFLN ELEEILDVIE 370 380 390 400 410 420 PSEFSKVMEP LFRQLAKCVS SPHFQVAERA LYYWNNEYIM SLISDNAARV LPIMFPALYR 430 440 450 460 470 480 NSKSHWNKTI HGLIYNALKL FMEMNQKLFD DCTQQYKAEK QKGRFRMKER EEMWQKIEEL 490 500 510 520 530 540 ARLNPQYPMF RAPPPLPPVY SMETETPTAE DIQLLKRTVE TEAVQMLKDI KKEKVLLRRK 550 560 570 SELPQDVYTI KALEAHKRAE EFLTASQEAL
	Isoform Delta-3 [UniParc]. Checksum: A57661F8B20731CB		496	58,453
	10 20 30 40 50 60 MPYKLKKEKE LFIQKLRQCC VLFDFVSDPL SDLKFKEVKR AGLNEMVEYI THSRDVVTEA 70 80 90 100 110 120 IYPEAVTMFS VNLFRTLPPS SNPTGAEFDP EEDEPTLEAA WPHLQLVYEF FLRFLESPDF 130 140 150 160 170 180 QPNIAKKYID QKFVLALLDL FDSEDPRERD FLKTILHRIY GKFLGLRAYI RRQINHIFYR 190 200 210 220 230 240 FIYETEHHNG IAELLEILGS IINGFALPLK EEHKMFLIRV LLPLHKVKSL SVYHPQLAYC 250 260 270 280 290 300 VVQFLEKESS LTEPVIVGLL KFWPKTHSPK EVMFLNELEE ILDVIEPSEF SKVMEPLFRQ 310 320 330 340 350 360 LAKCVSSPHF QVAERALYYW NNEYIMSLIS DNAARVLPIM FPALYRNSKS HWNKTIHGLI 370 380 390 400 410 420 YNALKLFMEM NQKLFDDCTQ QYKAEKQKGR FRMKEREEMW QKIEELARLN PQYPMFRAPP 430 440 450 460 470 480 PLPPVYSMET ETPTAEDIQL LKRTVETEAV QMLKDIKKEK VLLRRKSELP QDVYTIKALE 490 AHKRAEEFLT ASQEAL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The B56 family of protein phosphatase 2A (PP2A) regulatory subunits encodes differentiation-induced phosphoproteins that target PP2A to both nucleus and cytoplasm." McCright B., Rivers A.M., Audlin S., Virshup D.M. J. Biol. Chem. 271:22081-22089(1996) [PubMed: 8703017] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM DELTA-1). Tissue: Fetal brain.
[2]	"Molecular heterogeneity of the cDNA encoding a 74-kDa regulatory subunit (B'' or delta) of human protein phosphatase 2A." Tanabe O., Gomez G.A., Nishito Y., Usui H., Takeda M. FEBS Lett. 408:52-56(1997) [PubMed: 9180267] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS DELTA-1 AND DELTA-3). Tissue: Brain cortex.
[3]	"Molecular cloning of a 74-kDa regulatory subunit (B'' or delta) of human protein phosphatase 2A." Tanabe O., Nagase T., Murakami T., Nozaki H., Usui H., Nishito Y., Hayashi H., Kagamiyama H., Takeda M. FEBS Lett. 379:107-111(1996) [PubMed: 8566219] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM DELTA-2), PROTEIN SEQUENCE OF 501-508; 550-559; 573-580 AND 584-601 (DELTA-1). Tissue: Bone marrow and Brain cortex.
[4]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed: 14574404] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS DELTA-1 AND DELTA-2). Tissue: Colon, Eye and Kidney.
[7]	"Phosphoproteome analysis of the human mitotic spindle." Nousiainen M., Sillje H.H.W., Sauer G., Nigg E.A., Koerner R. Proc. Natl. Acad. Sci. U.S.A. 103:5391-5396(2006) [PubMed: 16565220] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-598, MASS SPECTROMETRY. Tissue: Epithelium.
[8]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed: 17525332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-598, MASS SPECTROMETRY.
[9]	"Automated phosphoproteome analysis for cultured cancer cells by two-dimensional nanoLC-MS using a calcined titania/C18 biphasic column." Imami K., Sugiyama N., Kyono Y., Tomita M., Ishihama Y. Anal. Sci. 24:161-166(2008) [PubMed: 18187866] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-553, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L76702 mRNA. Translation: AAB69751.1. AB000634 mRNA. Translation: BAA20381.1. AB000635 mRNA. Translation: BAA20382.1. D78360 mRNA. Translation: BAA11372.1. AL136304 Genomic DNA. Translation: CAI19791.1. CH471081 Genomic DNA. Translation: EAX04133.1. BC010692 mRNA. Translation: AAH10692.1. BC001095 mRNA. Translation: AAH01095.1. BC001175 mRNA. Translation: AAH01175.1.
PIR	S68686.
RefSeq	NP_006236.1. NP_851307.1. NP_851308.1.
UniGene	Hs.533308
3D structure databases
SMR	Q14738. Positions 106-482.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q14738.
PTM databases
PhosphoSite	Q14738.
Genome annotation databases
Ensembl	ENSG00000112640. Homo sapiens. [Contig view]
GeneID	5528.
KEGG	hsa:5528.
Organism-specific databases
H-InvDB	HIX0005889.
HGNC	HGNC:9312. PPP2R5D.
MIM	601646. gene.
PharmGKB	PA33676.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOGENOM	Q14738.
HOVERGEN	Q14738.
Enzyme and pathway databases
Reactome	REACT_11045. Signaling by Wnt. REACT_1505. Integration of energy metabolism.
Gene expression databases
CleanEx	HS_PPP2R5D.
GermOnline	ENSG00000112640. Homo sapiens.
Family and domain databases
InterPro	IPR002554. PP2A_B56. [Graphical view]
PANTHER	PTHR10257. B56. 1 hit.
Pfam	PF01603. B56. 1 hit. [Graphical view]
PIRSF	PIRSF028043. PP2A_B56. 1 hit.
BLOCKS	Search...
ProtoNet	Search...
Other Resources
LinkHub	Q14738.
NextBio	21410.
SOURCE	Search...

Entry information

Entry name

2A5D_HUMAN

Accession

Primary (citable) accession number: Q14738
Secondary accession number(s): O00494 , O00696, Q15171, Q5TC39

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1996
Last modified:	November 4, 2008
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)