Reviewed, UniProtKB/Swiss-Prot Q14773 (ICAM4_HUMAN)

Names and origin

Protein names

Recommended name:
    Intercellular adhesion molecule 4
      Short name=ICAM-4
Alternative name(s):
    Landsteiner-Wiener blood group glycoprotein
      Short name=LW blood group protein
    CD_antigen=CD242

Gene names

Name:	ICAM4
Synonyms:	LW

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	271 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM4 is also a ligand for alpha-4/beta-1 and alpha-V integrins.
Subcellular location	Isoform Long: Cell membrane; Single-pass type I membrane protein. Isoform Short: Secreted.
Tissue specificity	Erythrocytes.
Post-translational modification	N- and O-glycosylated.
Polymorphism	Responsible for the Landsteiner-Wiener blood group system. The molecular basis of the LW(A)=LW5/LW(B)=LW7 blood group antigens is a single variation in position 100; Gln-100 corresponds to LW(A) and Arg-100 to LW(B).
Sequence similarities	Belongs to the immunoglobulin superfamily. ICAM family. Contains 2 Ig-like C2-type (immunoglobulin-like) domains.

Ontologies

Keywords
Biological process	Cell adhesion
Cellular component	Cell membrane Membrane Secreted
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Immunoglobulin domain Repeat Signal Transmembrane
Molecular function	Blood group antigen
PTM	Glycoprotein
Gene Ontology (GO)
Cellular component	integral to membrane Traceable author statement. Source: ProtInc plasma membrane Traceable author statement. Source: ProtInc
Molecular function	integrin binding Traceable author statement. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform Long (identifier: Q14773-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Short (identifier: Q14773-2)

The sequence of this isoform differs from the canonical sequence as follows:
     233-271: AWSPAPTALASGSIAALVGILLTVGAAYLCKCLAMKSQA → GEAPL

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 271	249	Intercellular adhesion molecule 4
Regions
	Topological domain	23 – 240	218	Extracellular Potential
	Transmembrane	241 – 261	21	Potential
	Topological domain	262 – 271	10	Cytoplasmic Potential
	Domain	62 – 124	63	Ig-like C2-type 1
	Domain	146 – 217	72	Ig-like C2-type 2
Amino acid modifications
	Glycosylation	68	1	N-linked (GlcNAc...) Potential
	Glycosylation	78	1	N-linked (GlcNAc...) Potential
	Glycosylation	190	1	N-linked (GlcNAc...) Potential
	Glycosylation	223	1	N-linked (GlcNAc...) Potential
	Disulfide bond	69 ↔ 117		By similarity
	Disulfide bond	153 ↔ 210		By similarity
Natural variations
	Alternative sequence	233 – 271	39	AWSPA…MKSQA → GEAPL in isoform Short.
	Natural variant	100	1	Q → R in LW(B).
	Natural variant	208	1	V → L
Experimental info
	Sequence conflict	14 – 29	16	AAAYP…GRRTK → RPPTRELGARWDAGL in AAA59538 and AAA59537. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform Long [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 2F6BC8BABD79E615	FASTA	271	29,265
	10 20 30 40 50 60 MGSLFPLSLL FFLAAAYPGV GSALGRRTKR AQSPKGSPLA PSGTSVPFWV RMSPEFVAVQ 70 80 90 100 110 120 PGKSVQLNCS NSCPQPQNSS LRTPLRQGKT LRGPGWVSYQ LLDVRAWSSL AHCLVTCAGK 130 140 150 160 170 180 TRWATSRITA YKPPHSVILE PPVLKGRKYT LRCHVTQVFP VGYLVVTLRH GSRVIYSESL 190 200 210 220 230 240 ERFTGLDLAN VTLTYEFAAG PRDFWQPVIC HARLNLDGLV VRNSSAPITL MLAWSPAPTA 250 260 270 LASGSIAALV GILLTVGAAY LCKCLAMKSQ A
	Isoform Short [UniParc]. Checksum: F74416D3FB52ADB2		237	25,931
	10 20 30 40 50 60 MGSLFPLSLL FFLAAAYPGV GSALGRRTKR AQSPKGSPLA PSGTSVPFWV RMSPEFVAVQ 70 80 90 100 110 120 PGKSVQLNCS NSCPQPQNSS LRTPLRQGKT LRGPGWVSYQ LLDVRAWSSL AHCLVTCAGK 130 140 150 160 170 180 TRWATSRITA YKPPHSVILE PPVLKGRKYT LRCHVTQVFP VGYLVVTLRH GSRVIYSESL 190 200 210 220 230 ERFTGLDLAN VTLTYEFAAG PRDFWQPVIC HARLNLDGLV VRNSSAPITL MLGEAPL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The LW blood group glycoprotein is homologous to intercellular adhesion molecules." Bailly P., Hermand P., Callebaut I., Sonneborn H.H., Khamlichi S., Mornon J.-P., Cartron J.-P. Proc. Natl. Acad. Sci. U.S.A. 91:5306-5310(1994) [PubMed: 8202485] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT).
[2]	"Characterization of the gene encoding the human LW blood group protein in LW+ and LW- phenotypes." Hermand P., le Pennec P.Y., Rouger P., Cartron J.-P., Bailly P. Blood 87:2962-2967(1996) [PubMed: 8639917] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM LONG).
[3]	SeattleSNPs program for genomic applications Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT LEU-208.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG). Tissue: Lung.
[5]	"Molecular basis and expression of the LWa/LWb blood group polymorphism." Hermand P., Gane P., Mattei M.-G., Sistonen P., Cartron J.-P., Bailly P. Blood 86:1590-1594(1995) [PubMed: 7632968] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-130, VARIANT BLOOD GROUP LW(B) ARG-100.
[6]	"Intercellular adhesion molecule-4 binds alpha(4)beta(1) and alpha(V)-family integrins through novel integrin-binding mechanisms." Spring F.A., Parsons S.F., Ortlepp S., Olsson M.L., Sessions R., Brady R.L., Anstee D.J. Blood 98:458-466(2001) [PubMed: 11435317] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Web resources

BGMUT

Blood group antigen gene mutation database"

SeattleSNPs

Cross-references

Sequence databases
EMBLGenBankDDBJ	L27671 mRNA. Translation: AAA59538.1. L27670 mRNA. Translation: AAA59537.1. X93093 Genomic DNA. Translation: CAA63646.1. DQ011692 Genomic DNA. Translation: AAY16986.1. BC029364 mRNA. Translation: AAH29364.1. S78852 mRNA. Translation: AAB35046.1.
PIR	I52612. I59300. I80159.
RefSeq	NP_001535.1. NP_071772.1.
UniGene	Hs.699174
3D structure databases
HSSP	HSSP built from PDB template 1IC1 based on UniProtKB P05362.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q14773.
Genome annotation databases
Ensembl	ENSG00000105371. Homo sapiens. [Contig view]
GeneID	3386.
Organism-specific databases
H-InvDB	HIX0014739.
HGNC	HGNC:5347. ICAM4.
MIM	111250. gene+phenotype.
PharmGKB	PA29595.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOVERGEN	Q14773.
Enzyme and pathway databases
Reactome	REACT_6900. Signaling in Immune System.
Gene expression databases
CleanEx	HS_ICAM4.
GermOnline	ENSG00000105371. Homo sapiens.
Family and domain databases
InterPro	IPR013768. ICAM_N. IPR003987. ICAM_VCAM_N. IPR007110. Ig-like. IPR013783. Ig-like_fold. [Graphical view]
Gene3D	G3DSA:2.60.40.10. Ig-like_fold. 2 hits.
Pfam	PF03921. ICAM_N. 1 hit. [Graphical view]
PRINTS	PR01472. ICAMVCAM1.
PROSITE	PS50835. IG_LIKE. False negative. [Graphical view]
ProDom	Q14773. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
SOURCE	Search...
ProtoNet	Search...

Entry information

Entry name

ICAM4_HUMAN

Accession

Primary (citable) accession number: Q14773
Secondary accession number(s): A0M8X2 , Q14771, Q14772, Q16375

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	July 22, 2008
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)