Reviewed, UniProtKB/Swiss-Prot Q14CH7 (SYAM_MOUSE)

Names and origin

Protein names

Recommended name:
    Probable alanyl-tRNA synthetase, mitochondrial
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name(s)=AlaRS
    Alanyl-tRNA synthetase-like

Gene names

Name:	Aars2
Synonyms:	Aarsl, Gm89, Kiaa1270

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	980 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala).
Subcellular location	Mitochondrion matrixBy similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Gene Ontology (GO)
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Transit peptide	1 – 23	23	Mitochondrion Potential
	Chain	24 – 980	957	Probable alanyl-tRNA synthetase, mitochondrial
Experimental info
	Sequence conflict	618 – 625	8	AWRMGCMV → RSRPG in BAD32417. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q14CH7-1 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: 7734BC58CDB5463D	FASTA	980	106,783
	10 20 30 40 50 60 MAVALAAAAG KLRRAIGRSC PWQPFSTEPG PPHGAAVRDA FLSFFRDRHG HRLVPSATVR 70 80 90 100 110 120 PRGDPSLLFV NAGMNQFKPI FLGTVDPRSE MAGFRRVVNS QKCVRAGGRH NDLEDVGRDL 130 140 150 160 170 180 SHHTFFEMLG NWAFGGEYFK EEACSMAWEL LTQVYGIPED RLWVSYFSGD SQTGLDPDLE 190 200 210 220 230 240 TRDIWLSLGV PASRVLSFGP QENFWEMGDT GPCGPCTEIH YDLAGGVGSP QLVELWNLVF 250 260 270 280 290 300 MQHYREADGS LQLLPQRHVD TGMGLERLVA VLQGKRSTYD TDLFSPLLDA IHQSCGAPPY 310 320 330 340 350 360 SGRVGAADEG RIDTAYRVVA DHIRTLSVCI ADGVSPGMSG APLVLRRILR RAVRYSTEVL 370 380 390 400 410 420 QAPPGFLGSL VPVVVETLGS AYPELEKNSV KIASLVSEDE AAFLASLQRG RRIIDRTVKR 430 440 450 460 470 480 LGPSDLFPAE VAWSLSLSGN LGIPLDLVEL MLEEKGVKLD TAGLEQLAQK EAQHRAQQAE 490 500 510 520 530 540 ADQEDRLCLD VHALEELHRQ GIPTTDDSPK YNYTLHPNGD YEFGLCEARV LQLYSETGTA 550 560 570 580 590 600 VASVGAGQRC GLLLDRTNFY AEQGGQASDR GYLVRTGQQD MLFPVAGAQL CGGFILHEAM 610 620 630 640 650 660 APERLQVGDQ VQLYVDKAWR MGCMVKHTAT HLLSWALRQT LGPTTEQRGS HLNPERLRFD 670 680 690 700 710 720 VATQTLLTTE QLRTVESYVQ EVVGQDKPVF MEEVPLAHTA RIPGLRSLDE VYPDPVRVVS 730 740 750 760 770 780 VGVPVAHALG PASQAAMHTS VELCCGTHLL STGAVGDLVI IGERQLVKGI TRLLAITGEQ 790 800 810 820 830 840 AQQAREVGQS LSQEVEAASE RLSQGSRDLP EAHRLSKDIG RLTEVAESAV IPQWQRQELQ 850 860 870 880 890 900 TTLKMLQRRA NTAIRKLEKG QATEKSQELL KRHSEGPLIV DTVSAESLSV LVKVVRQLCK 910 920 930 940 950 960 QAPSISVLLL SPQPTGSVLC ACQVAQDATP TFTAEAWALA VCSHMGGKAW GSRVVAQGTG 970 980 HTADLEAALG TARAYALSQL

References

[1]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.

[2]

"Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H. DNA Res. 11:205-218(2004) [PubMed: 15368895] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 242-980. Tissue: Embryonic intestine.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC079844 mRNA. Translation: AAH79844.1. BC113172 mRNA. Translation: AAI13173.1. BC113779 mRNA. Translation: AAI13780.1. AK173139 pre-RNA. Translation: BAD32417.1.
RefSeq	NP_941010.2.
UniGene	Mm.329063
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSMUSG00000023938. Mus musculus. [Contig view]
GeneID	224805.
KEGG	mmu:224805.
Organism-specific databases
MGI	MGI:2681839. Aars2.
Rouge	Search...
Phylogenomic databases
HOVERGEN	Q14CH7.
Gene expression databases
ArrayExpress	Q14CH7.
Family and domain databases
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProDom	Q14CH7. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
SOURCE	Search...
ProtoNet	Search...

Entry information

Entry name

SYAM_MOUSE

Accession

Primary (citable) accession number: Q14CH7
Secondary accession number(s): Q68FH3, Q69ZM9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 3, 2006
Last sequence update:	August 22, 2006
Last modified:	July 22, 2008
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)