Reviewed, UniProtKB/Swiss-Prot Q17802 (CPG1_CAEEL)

Names and origin

Protein names

Recommended name:
    Chondroitin proteoglycan 1
Alternative name(s):
    Cytokinesis protein cej-1
    Cell junction protein 1

Gene names

Name:	cpg-1
Synonyms:	cej-1
ORF Names:	C07G2.1

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	584 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Required for polar body extrusion during cytokinesis in embryo development. Affects cortical granule size. Has roles in meiotic chromosome segregation, osmotic barrier function and polarization in conjunction with cpg-2. Binds chitin.
Tissue specificity	Expressed in the germline.
Developmental stage	Expressed throughout development but appears to be up-regulated in adults.
Miscellaneous	Worms lacking cpg-1 and cpg-2 exhibit defects in cytokinesis during embryo development, more specifically meiotic chromosome segregation, polar-body extrusion, osmotic barrier function and polarization. Embryos lacking cpg-1 and cpg-2 proteins have multiple nuclei lacking plasma membranes and may also have weak egg shells. Oocytes lacking cpg-1 and cpg-2 show cortical granules that are reduced in size.
Sequence similarities	Contains 3 chitin-binding type-2 domains.

Ontologies

Keywords
Biological process	Cell cycle Cell division
Coding sequence diversity	Alternative splicing
Domain	Repeat Signal
Ligand	Chitin-binding
Molecular function	Developmental protein
PTM	Glycoprotein Proteoglycan
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chitin metabolic process Inferred from electronic annotation. Source: InterPro cytokinesis after mitosis Ref.4 Inferred from mutant phenotype. Source: UniProtKB multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: InterPro
Molecular function	chitin binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform a (identifier: Q17802-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform b (identifier: Q17802-2)

The sequence of this isoform differs from the canonical sequence as follows:
     124-547: Missing.

Notes: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Chain	18 – 584	567	Chondroitin proteoglycan 1		PRO_0000023616
Regions
	Domain	58 – 115	58	Chitin-binding type-2 1
	Domain	211 – 268	58	Chitin-binding type-2 2
	Domain	524 – 578	55	Chitin-binding type-2 3
Amino acid modifications
	Glycosylation	50	1	O-linked (Xyl...) (chondroitin sulfate)
	Glycosylation	268	1	N-linked (GlcNAc...) Potential
	Disulfide bond	91 ↔ 104		By similarity
	Disulfide bond	244 ↔ 257		By similarity
	Disulfide bond	554 ↔ 567		By similarity
Natural variations
	Alternative sequence	124 – 547	424	Missing in isoform b.		VSP_050569

Sequences

	Sequence		Length	Mass (Da)
	Isoform a [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 76F8F613E239DD53	FASTA	584	61,805
	10 20 30 40 50 60 MTLKPVLLAF LVASAYAQYG VAGMYENLPL ETTTLDGSGD GSGADNGFVS GADAVAIDTD 70 80 90 100 110 120 CSTKEDGLYA IGGCSPQFLT CSGGISRIMD CPADLIYDPR IVACEYSYNV PQCGGVPQDV 130 140 150 160 170 180 TSTQEAYPSE ETTVNPYAPV EEATTTPAED VTVPEETTTE AYAPVDDYST TTPAEDVPVP 190 200 210 220 230 240 VETTASPYAP IVPYTTGAPA ADEPVTRSAV TKSCVGKADG FYSFGECSDH YTACSNGYLI 250 260 270 280 290 300 PMQCPARLAF DEARVICDYV MNVPECTNGS GNDEGSADET TPESSGEMPY SNGYGYEETT 310 320 330 340 350 360 TVAEDVPSTK DYAEPIAAAY VARYPSEKTT AENVPTTTIG YEPEVVETTA PYVEETTTTV 370 380 390 400 410 420 GYKPEVEETT TEAEVPTTTV GYEPEIVETT APYVEETTTA ADVPSTTAVY EPEVVETTTE 430 440 450 460 470 480 AEVPTTTTVG YEPEVVETTV PYVEETTTAA DVPTTTVGYE PEVEETTTEA EVPTTTVGYE 490 500 510 520 530 540 SEVVETTAAD IPTTTIGYAP IVVESTTAAD VPTTTVPAET TTEVPACVEG ATAIEPCSQH 550 560 570 580 YKNCVNGQEA IFICENGLFF SPEQARCAPA DQIAECHQTT VQYY
	Isoform b [UniParc]. Checksum: 0C6F93A63073925C		160	16,897
	10 20 30 40 50 60 MTLKPVLLAF LVASAYAQYG VAGMYENLPL ETTTLDGSGD GSGADNGFVS GADAVAIDTD 70 80 90 100 110 120 CSTKEDGLYA IGGCSPQFLT CSGGISRIMD CPADLIYDPR IVACEYSYNV PQCGGVPQDV 130 140 150 160 TSTQEAIFIC ENGLFFSPEQ ARCAPADQIA ECHQTTVQYY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of novel chondroitin proteoglycans in Caenorhabditis elegans: embryonic cell division depends on CPG-1 and CPG-2." Olson S.K., Bishop J.R., Yates J.R., Oegema K., Esko J.D. J. Cell Biol. 173:985-994(2006) [PubMed: 16785326] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), IDENTIFICATION BY MASS SPECTROMETRY, GLYCOSYLATION AT SER-50, FUNCTION.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING. Strain: Bristol N2.
[3]	"A global profile of germline gene expression in C. elegans." Reinke V., Smith H.E., Nance J., Wang J., Van Doren C., Begley R., Jones S.J.M., Davis E.B., Scherer S., Ward S., Kim S.K. Mol. Cell 6:605-616(2000) [PubMed: 11030340] [Abstract] Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[4]	"Identification of in vivo mRNA targets of GLD-1, a maxi-KH motif containing protein required for C. elegans germ cell development." Lee M.-H., Schedl T. Genes Dev. 15:2408-2420(2001) [PubMed: 11562350] [Abstract] Cited for: FUNCTION.
[5]	"The eggshell is required for meiotic fidelity, polar-body extrusion and polarization of the C. elegans embryo." Johnston W.L., Krizus A., Dennis J.W. BMC Biol. 4:35-35(2006) [PubMed: 17042944] [Abstract] Cited for: FUNCTION.
[6]	"Cortical granule exocytosis in C. elegans is regulated by cell cycle components including separase." Bembenek J.N., Richie C.T., Squirrell J.M., Campbell J.M., Eliceiri K.W., Poteryaev D., Spang A., Golden A., White J.G. Development 134:3837-3848(2007) [PubMed: 17913784] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	DQ340623 mRNA. Translation: ABC65811.1. Z32840 Genomic DNA. Translation: CAA83679.1. Z32840 Genomic DNA. Translation: CAD91625.1.
PIR	T19061.
RefSeq	NP_001021159.1.
UniGene	Cel.23350
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:27081N.
IntAct	Q17802.
Genome annotation databases
Ensembl	C07G2.1. Caenorhabditis elegans. [Contig view]
GeneID	175586.
KEGG	cel:C07G2.1.
Organism-specific databases
WormBase	WBGene00000465. cpg-1.
WormPep	C07G2.1a. CE00665. [WorfDB] C07G2.1b. CE33971. [WorfDB]
Gene expression databases
ArrayExpress	Q17802.
Family and domain databases
InterPro	IPR002557. Chitin-bd_peritrophin-A. [Graphical view]
Pfam	PF01607. CBM_14. 3 hits. [Graphical view]
SMART	SM00494. ChtBD2. 3 hits. [Graphical view]
PROSITE	PS50940. CHIT_BIND_II. 3 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	888782.

Entry information

Entry name

CPG1_CAEEL

Accession

Primary (citable) accession number: Q17802
Secondary accession number(s): Q1A3T6

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 15, 2003
Last sequence update:	November 1, 1996
Last modified:	November 25, 2008
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project