Reviewed, UniProtKB/Swiss-Prot Q1JEZ3 (LACG_STRPD)

Names and origin

Protein names

Recommended name:
    6-phospho-beta-galactosidase
    EC=3.2.1.85
Alternative name(s):
    Beta-D-phosphogalactoside galactohydrolase
      Short name=PGALase
    P-beta-Gal
      Short name=PBG

Gene names

Name:	lacG
Ordered Locus Names:	MGAS10270_Spy1701

Organism

Streptococcus pyogenes serotype M2 (strain MGAS10270) [Complete proteome] [HAMAP]

Taxonomic identifier

370552 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	468 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	A 6-phospho-beta-D-galactoside + H(2)O = 6-phospho-D-galactose + an alcohol.
Pathway	Carbohydrate metabolism; lactose degradation; D-galactose 6-phosphate and beta-D-glucose from lactose 6-phosphate: step 1/1.
Sequence similarities	Belongs to the glycosyl hydrolase 1 family.

Ontologies

Keywords
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	galactosidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 468	468	6-phospho-beta-galactosidase
Sites
	Active site	160	1	Proton donor By similarity
	Active site	375	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1JEZ3-1 [UniParc]. Last modified June 13, 2006. Version 1. Checksum: AAA9E2599AF2714C	FASTA	468	53,791
	10 20 30 40 50 60 MTKTLPKDFI FGGATAAYQA EGATHTDGKG PVAWDKYLED NYWYTAEPAS DFYNRYPVDL 70 80 90 100 110 120 KLSEEFGVNG IRISIAWSRI FPTGKGEVNP KGVEYYHNLF AECHKRHVEP FVTLHHFDTP 130 140 150 160 170 180 EALHSDGDFL NRENIEHFVN YAEFCFKEFS EVNYWTTFNE IGPIGDGQYL VGKFPPGIQY 190 200 210 220 230 240 DLAKVFQSHH NMMVSHARAV KLFKDGGYSG EIGVVHALPT KYPFDANNPD DVRAAELEDI 250 260 270 280 290 300 IHNKFILDAT YLGKYSDKTM EGVNHILEVN GGELDLCEED FAALDAAKDL NDFLGINYYM 310 320 330 340 350 360 SDWMQAFDGE TEIIHNGKGE KGSSKYQIKG VGRRKAPVDV PKTDWDWILF PQGLYDQIMR 370 380 390 400 410 420 VKADYPNYKK IYITENGLGY KDEFVDNTVY DDGRIDYVKK HLEVISDAIS DGVNVKGYFM 430 440 450 460 WSLMDVFSWS NGYEKRYGLF YVDFETQERY PKKSAYWYKK VAETQVIE

References

[1]

"Molecular genetic anatomy of inter- and intraserotype variation in the human bacterial pathogen group A Streptococcus." Beres S.B., Richter E.W., Nagiec M.J., Sumby P., Porcella S.F., DeLeo F.R., Musser J.M. Proc. Natl. Acad. Sci. U.S.A. 103:7059-7064(2006) [PubMed: 16636287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000260 Genomic DNA. Translation: ABF34766.1.
RefSeq	YP_599310.1.
3D structure databases
SMR	Q1JEZ3. Positions 1-468.
ModBase	Search...
Genome annotation databases
GeneID	4062536.
GenomeReviews	Gene locus lacG in contig CP000260_GR.
KEGG	sph:MGAS10270_Spy1701.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q1JEZ3.
Enzyme and pathway databases
BioCyc	SPYO370552:MGAS10270_SPY1701-MON.
Family and domain databases
HAMAP	MF_01574. [Tree]
InterPro	IPR001360. Glyco_hydro_1. IPR013781. Glyco_hydro_sub_cat. IPR005928. LacG. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHER	PTHR10353. Glyco_hydro_1. 1 hit.
Pfam	PF00232. Glyco_hydro_1. 1 hit. [Graphical view]
PRINTS	PR00131. GLHYDRLASE1.
TIGRFAMs	TIGR01233. lacG. 1 hit.
PROSITE	PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit. PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit. [Graphical view]
ProDom	Q1JEZ3. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

LACG_STRPD

Accession

Primary (citable) accession number: Q1JEZ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	June 13, 2006
Last modified:	September 2, 2008
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)