Reviewed, UniProtKB/Swiss-Prot Q1ZXQ8 (MTAA_DICDI)

Names and origin

Protein names

Recommended name:
    Centrosomal protein 224
      Short name=CP224

Gene names

Name:	mtaA
ORF Names:	DDB_0232951

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	2013 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in regulation of microtubule dynamics. Regulates the interaction of microtubules tips with the centrosome and cell cortex.
Subunit structure	Interacts with eb1 at the microtubule tip, centrosome and kinetochore. Interacts with lis1 in the cortical attachment of microtubules.
Subcellular location	Centrosome. Cytoplasm › cytoskeleton. Kinetochore. Note= Associated with the microtubule network. Accumulates at the plus end of microtubules.
Developmental stage	Expressed throughout the cell cycle (at protein level).
Miscellaneous	Mutants overexpressing mtaA exhibit supernumerary centrosomes and a cytokinesis defect. Mutants underexpressing mtaA display inhibited growth and a reduction in the number and length of astral microtubes. Mutants underexpressing mtaA exhibited a disappearence of supernumerary centrosomes and multinuclear cells, and increased sensitivity to the microtubule depolymerizing drug nocodazole.
Sequence similarities	Belongs to the TOG/XMAP215 family. Contains 13 HEAT repeats.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Cytoplasm Cytoskeleton Kinetochore Microtubule
Domain	Repeat
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	Golgi organization and biogenesis Ref.6 Inferred from mutant phenotype. Source: dictyBase centrosome duplication Ref.1 Inferred from mutant phenotype. Source: dictyBase cytokinesis Ref.1 Inferred from mutant phenotype. Source: dictyBase microtubule polymerization Ref.5 Inferred from mutant phenotype. Source: dictyBase
Cellular component	cell cortex Ref.6 Inferred from direct assay. Source: dictyBase centrosome Ref.1 Ref.6 Inferred from direct assay. Source: dictyBase endocytic vesicle Ref.6 Inferred from direct assay. Source: dictyBase microtubule Ref.4 Inferred from direct assay. Source: dictyBase
Molecular function	dynein binding Ref.6 Inferred from direct assay. Source: dictyBase microtubule binding Ref.4 Inferred from direct assay. Source: dictyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 2013	2013	Centrosomal protein 224
Regions
	Repeat	115 – 153	39	HEAT 1
	Repeat	158 – 196	39	HEAT 2
	Repeat	200 – 238	39	HEAT 3
	Repeat	348 – 386	39	HEAT 4
	Repeat	427 – 465	39	HEAT 5
	Repeat	724 – 762	39	HEAT 6
	Repeat	816 – 854	39	HEAT 7
	Repeat	857 – 895	39	HEAT 8
	Repeat	899 – 937	39	HEAT 9
	Repeat	977 – 1015	39	HEAT 10
	Repeat	1240 – 1279	40	HEAT 11
	Repeat	1281 – 1314	34	HEAT 12
	Repeat	1317 – 1353	37	HEAT 13
	Compositional bias	544 – 549	6	Poly-Pro
	Compositional bias	1057 – 1077	21	Ser-rich
	Compositional bias	1083 – 1091	9	Poly-Gln
	Compositional bias	1094 – 1103	10	Poly-Gln
	Compositional bias	1372 – 1391	20	Poly-Asn
	Compositional bias	1393 – 1406	14	Poly-Gln
	Compositional bias	1703 – 1708	6	Poly-Asn
	Compositional bias	1717 – 1721	5	Poly-Asn
	Compositional bias	1749 – 1796	48	Thr-rich
	Compositional bias	1766 – 1769	4	Poly-Gln
	Compositional bias	1913 – 1939	27	Poly-Asn
	Compositional bias	1976 – 1979	4	Poly-Asn
Experimental info
	Sequence conflict	1391	1	N → NN in CAB56504. Ref.1
	Sequence conflict	1929	1	N → NN in CAB56504. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q1ZXQ8-1 [UniParc]. Last modified May 2, 2006. Version 1. Checksum: D0F9FD82D89EC2C4	FASTA	2,013	223,812
	10 20 30 40 50 60 MGDEEAPSGS IEDRINHKNW KWRVSGLEEL TTKFRNSIEG SGPLFNEWGP QFKKILADIN 70 80 90 100 110 120 PMSQERALEP LSAFIDRCDC VNKFAASYVG VLVEKLFAST RPRAKEKTIE CLLLTIEADS 130 140 150 160 170 180 AEPVVEALLK GTSSTSPKIL LASLAALTQA LKTFGPKQIP VKLILKQFSP WFENRDKGIR 190 200 210 220 230 240 DQASELFIEI YRWIGKALIP LISEALTPIQ LKALQDQFEK LPTDPAVPLK YTRSEAAKAL 250 260 270 280 290 300 ANASKGIQAK PEVVEEIDPY SLMTAVNILP KLTSEFYEGL QAKKWQERSE QMDKLVTILT 310 320 330 340 350 360 NTPKIETADF SELCKALKKI LADVNVMIVQ KAVVSIGLLA DSLRGGFTSY VKPFITPILE 370 380 390 400 410 420 KFKEKKTSVL QSVHTTMDSL VGKSISLSDI IDELTATMQS KVPQIKQEVL VFICNSITNT 430 440 450 460 470 480 KKPADITKVT KQLTKIFMEA LNDTDSNIRD NASKAFAALG GIIGERAMTP YLNQIDPIKA 490 500 510 520 530 540 KKIKDNMPAV ATPVTITPQP LAPVDLKDID LPVSSSNKKP AAATGNSKSS STTTPTGRSS 550 560 570 580 590 600 NSSPLPPPPS SSDDIKNKLI GAGIVNNEII EGLGKTQWKD RLQAVDDILE NVKGLTADSI 610 620 630 640 650 660 NGMSESIIQL LCDKPSLKES NFQVLSSIFS IFIQCCKNDS NFTQQRCANS YLTTCIEKLT 670 680 690 700 710 720 DVKLKEISSE LLFSTGESIT PHAVFTSIYQ FTSNHKNPKI IADSLVWIQQ AIDEFGIGCC 730 740 750 760 770 780 SNGIQQLKPL LDYTKQCLES TNPDVKKSAI KLLCTIKINI GATLTDFLGD VKKPTMEVLD 790 800 810 820 830 840 REFQKIRDQK PPVPNRQWKG MPPPGSAPVQ IEFPRVDISV KLTPAIITNL SDANWKTRSD 850 860 870 880 890 900 ALDEIERIII DANRKIQPKL GGLIPALKNR LTDNNQKCTI TTLNIIGMLS QAMGGQSFEK 910 920 930 940 950 960 HARLLIPGIL LLLGDSKKPV RDAVISCMNV IVQSDLGFDV FIGSLAAPMI QESAFTRKES 970 980 990 1000 1010 1020 LAWTIVNVTN MKAAPIPSEI NTLAKGIISC LQDKSAEIRS LADNLLSILC TQIPLIEFKK 1030 1040 1050 1060 1070 1080 ELKHVKPASQ PSIQTILDKY YQKTGQPIPP PSKTKQSTSS SSSSSSTTSQ QSSTPSSPQP 1090 1100 1110 1120 1130 1140 IRQQQQQQQQ QPTQPQQQQQ QQQRRSILQS NNGASNCEFI IFDINGKMNR QKTNQIPSWH 1150 1160 1170 1180 1190 1200 FIEPTEEVVE ILQDQVLQCF TEEFANLMFS SLPSNSQHMS DLMIGMIEQN PEAIISVLDI 1210 1220 1230 1240 1250 1260 LFRWITFKLF DTGLASQKRV LKILEILLNK LIDSEYSIGE YEASCLVPIL LEKSGSATNE 1270 1280 1290 1300 1310 1320 QIKQIFKQSI QQLEELCLPN VLFRFAIEMV TSQNWRTRVE VLNVMASIID KNGASVCGNL 1330 1340 1350 1360 1370 1380 KVVIPLITQN LNDSQSKQSS LLCLNKLYSH IKDECFKYSN ISQQDKILII GNNNNNNNNN 1390 1400 1410 1420 1430 1440 NNNNNNNNNN NVQQQQQQQQ QQQQQQPRKS LSTDEMSTQL IGCLELLKNY SITKENIEHT 1450 1460 1470 1480 1490 1500 VEALKQFSGL MANGKLDDVF VNFAEEYFLV LTSILADTFP QVSKDATILR LCKYLIHTII 1510 1520 1530 1540 1550 1560 SILSNKVVAK QCNVRCLEIV LNETIKLYSL AESNSSKQGT ESELSKAFNQ ILLRILQNCN 1570 1580 1590 1600 1610 1620 STILFSTLLQ MMSRTDNDQS IQHPGKYNDL LLRCLLRATK SLTTPSILEE LNVETVLSEI 1630 1640 1650 1660 1670 1680 NSFLKSNPSL DEITRKTTKT LTSELYQNRT NQVVKFTKDI ISKGQQQKYQ YLLNLLIELV 1690 1700 1710 1720 1730 1740 PKQFEDLING NGNGNRISSG GSNNNNNNGG RVSSGGNNNN NSGRENINNI NINSSLDSPI 1750 1760 1770 1780 1790 1800 NDHHNSNNTT TTTTRSRTST GSSNSQQQQL PTTSANTTTT TTTTTTTGKT QSTLSTLKIN 1810 1820 1830 1840 1850 1860 KEPRDYSGKT DSQKKELLIE IFKKIGNKDL TLDGIHDLYF FIREYPDYDI TPNLNSSSQQ 1870 1880 1890 1900 1910 1920 FQAYITRNLK KIKDSMDAPK DKDDEVVNYQ ERLKVIQNTF YNQHNQPSSQ VVNNNNNNNN 1930 1940 1950 1960 1970 1980 NNNNNNNNNI NNNNNNNNNS GGNENIAPQL SNTASMASNT LQRLRTLNPD QNSGSNNNNS 1990 2000 2010 HQNSPSTSSS NDLNSTVASL RQRLAQLTSK SNE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Dictyostelium DdCP224 is a microtubule-associated protein and a permanent centrosomal resident involved in centrosome duplication." Graef R., Daunderer C., Schliwa M. J. Cell Sci. 113:1747-1758(2000) [PubMed: 10769206] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE. Strain: AX2.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[3]	"Centrosomal microtubule plus end tracking proteins and their role in Dictyostelium cell dynamics." Hestermann A., Rehberg M., Graef R. J. Muscle Res. Cell Motil. 23:621-630(2002) [PubMed: 12952061] [Abstract] Cited for: FUNCTION, SUBUNIT.
[4]	"Dictyostelium EB1 is a genuine centrosomal component required for proper spindle formation." Rehberg M., Graef R. Mol. Biol. Cell 13:2301-2310(2002) [PubMed: 12134070] [Abstract] Cited for: SUBUNIT, SUBCELLULAR LOCATION.
[5]	"Regulated expression of the centrosomal protein DdCP224 affects microtubule dynamics and reveals mechanisms for the control of supernumerary centrosome number." Graef R., Euteneuer U., Ho T.H., Rehberg M. Mol. Biol. Cell 14:4067-4074(2003) [PubMed: 14517319] [Abstract] Cited for: FUNCTION.
[6]	"The XMAP215-family protein DdCP224 is required for cortical interactions of microtubules." Hestermann A., Graef R. BMC Cell Biol. 5:24-24(2004) [PubMed: 15186508] [Abstract] Cited for: FUNCTION.
[7]	"Dictyostelium LIS1 is a centrosomal protein required for microtubule/cell cortex interactions, nucleus/centrosome linkage, and actin dynamics." Rehberg M., Kleylein-Sohn J., Faix J., Ho T.-H., Schulz I., Graef R. Mol. Biol. Cell 16:2759-2771(2005) [PubMed: 15800059] [Abstract] Cited for: SUBUNIT.
[8]	"Identification and isolation of Dictyostelium microtubule-associated protein interactors by tandem affinity purification." Koch K.V., Reinders Y., Ho T.-H., Sickmann A., Graef R. Eur. J. Cell Biol. 85:1079-1090(2006) [PubMed: 16782229] [Abstract] Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ012088 mRNA. Translation: CAB56504.1. AAFI02000004 Genomic DNA. Translation: EAS66944.1.
RefSeq	XP_001134481.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4238023.
KEGG	ddi:DDB_0232951.
Organism-specific databases
dictyBase	DDB0232951. mtaA.
Family and domain databases
InterPro	IPR000357. HEAT. [Graphical view]
Pfam	PF02985. HEAT. 3 hits. [Graphical view]
PROSITE	PS50077. HEAT_REPEAT. 1 hit. [Graphical view]
ProDom	Q1ZXQ8. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

MTAA_DICDI

Accession

Primary (citable) accession number: Q1ZXQ8
Secondary accession number(s): Q9U5Y1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 2, 2006
Last modified:	July 22, 2008
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)