Reviewed, UniProtKB/Swiss-Prot Q27333 (NT56_DROME)

Names and origin

Protein names

Recommended name:
    Lethal(2)neighbour of tid protein
Alternative name(s):
    NOT56
    NOT45

Gene names

Name:	l(2)not
ORF Names:	CG4084

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	510 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane proteinPotential.
Developmental stage	Expressed only in embryos.
Miscellaneous	In strains Harvich and Apxo, there are 2 genes which code for l(2)not protein. They differ in their C-terminus.
Sequence similarities	Belongs to the glycosyltransferase 58 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane
Molecular function	Developmental protein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: InterPro
Molecular function	mannosyltransferase activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	Q9VMD4	1	EBI-173443,EBI-86395

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 510	510	Lethal(2)neighbour of tid protein		PRO_0000080568
Regions
	Transmembrane	44 – 64	21	Potential
	Transmembrane	98 – 118	21	Potential
	Transmembrane	126 – 146	21	Potential
	Transmembrane	172 – 192	21	Potential
	Transmembrane	212 – 232	21	Potential
	Transmembrane	234 – 254	21	Potential
	Transmembrane	295 – 315	21	Potential
	Transmembrane	404 – 424	21	Potential
	Transmembrane	427 – 447	21	Potential
	Transmembrane	465 – 485	21	Potential
Natural variations
	Natural variant	286	1	V → L in strain: Berkeley and bIf.
	Natural variant	311	1	I → T in strain: Berkeley, bIf and Apxo.
	Natural variant	333	1	T → A in strain: Berkeley, bIf and Apxo.
	Natural variant	338	1	Q → E in strain: Berkeley, bIf and Apxo.
	Natural variant	340	1	K → N in strain: Berkeley and bIf.
	Natural variant	489	1	I → V in strain: Berkeley, bIf and Apxo.
Experimental info
	Mutagenesis	433	1	H → R in tid4 mutant

Sequences

	Sequence		Length	Mass (Da)
	Q27333-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: E2F7415940465F25	FASTA	510	58,461
	10 20 30 40 50 60 MAPPKAASHR PAVRRKKSGT LVDSILDKYL NVRFFKYLLL EPAALPIVGL FVLLAELVIN 70 80 90 100 110 120 VVVIQRVPYT EIDWVAYMQE CEGFLNGTTN YSLLRGDTGP LVYPAAFVYI YSALYYVTSH 130 140 150 160 170 180 GTNVRLAQYI FAGIYLLQLA LVLRLYSKSR KVPPYVLVLS AFTSYRIHSI YVLRLFNDPV 190 200 210 220 230 240 AVLLLYAALN LFLDRRWTLG STFFSLAVGV KMNILLFAPA LLLFYLANLG LLRTILQLAV 250 260 270 280 290 300 CGVIQLLLGA PFLLTHPVEY LRGSFDLGRI FEHKWTVNYR FLSRDVFENR TFHVSLLGLH 310 320 330 340 350 360 LLLLLAFAKP IWTFFQSYVR LRRIEDQLQP QITQQNLQLK AQKRPKKVEK DKDKDQKKFT 370 380 390 400 410 420 TEQQSFLKAF EKSLQKASGG KATPAPAQAE PERYGIHFDR CTQLALLPFF LCNLVGVACS 430 440 450 460 470 480 RSLHYQFYVW YFHSLPYLAW STPYSLGVRC LILGLIEYCW NTYPSTNFSS AALHFTHIIL 490 500 510 LAGVAKQLIQ TMRINNAAKR EQQEQQKKLQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Gene within gene configuration and expression of the Drosophila melanogaster genes lethal(2) neighbour of tid [l(2)not] and lethal(2) relative of tid." Kurzik-Dumke U., Kaymer M., Gundacker D., Debes A., Labitzke K. Gene 200:45-58(1997) [PubMed: 9373138] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], CHARACTERIZATION. Strain: Apxo, bIf, Harvich and Oregon-R. Tissue: Embryo.
[2]	"Sequence of a 10291 nt genomic region of Drosophila melanogaster harbouring the genes l(2)tid, l(2)not, l(2)rot, and l(2)dtl." Gundacker S., Neubhuer M., Kurzik-Dumke U. Submitted (MAY-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: Oregon-2.
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[5]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 179-510. Strain: Berkeley. Tissue: Embryo.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X95241 Genomic DNA. Translation: CAA64529.1. X77820 mRNA. Translation: CAA54835.1. X95248 Genomic DNA. Translation: CAA64537.1. X95252 Genomic DNA. Translation: CAA64541.1. X95243 Genomic DNA. Translation: CAA64532.1. Y10074 Genomic DNA. Translation: CAA71167.1. X98094 Genomic DNA. Translation: CAA66721.1. AE013599 Genomic DNA. Translation: AAF47050.1. AY061582 mRNA. Translation: AAL29130.1. Different initiation.
PIR	S42090.
RefSeq	NP_523829.2.
UniGene	Dm.2569
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q27333.
Genome annotation databases
Ensembl	CG4084. Drosophila melanogaster. [Contig view]
GeneID	37754.
KEGG	dme:Dmel_CG4084.
Organism-specific databases
FlyBase	FBgn0011297. l(2)not.
Gene expression databases
ArrayExpress	Q27333.
GermOnline	CG4084. Drosophila melanogaster.
Family and domain databases
InterPro	IPR007873. ALG3. [Graphical view]
PANTHER	PTHR12646. ALG3. 1 hit.
Pfam	PF05208. ALG3. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	805248.

Entry information

Entry name

NT56_DROME

Accession

Primary (citable) accession number: Q27333
Secondary accession number(s): O02532 , O02533, Q24419, Q24420, Q24421, Q24607, Q95R76, Q9W1K8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	November 25, 2008
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project