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Reviewed, UniProtKB/Swiss-Prot Q34312 (NDUS1_DICDI)

Last modified September 2, 2008. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    NADH-ubiquinone oxidoreductase 75 kDa subunit
    EC=1.6.5.3
    EC=1.6.99.3
Alternative name(s):
    Complex I-75kD
      Short name=CI-75kD
Gene names
Name: nad11
Synonyms: ndufs1
ORF Names: DDB_0201618
Encoded onMitochondrion
OrganismDictyostelium discoideum (Slime mold) [Complete proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryota › Mycetozoa › Dictyosteliida › Dictyostelium

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Protein attributes

Sequence length688 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity. This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized By similarity.

Catalytic activity

NADH + ubiquinone = NAD(+) + ubiquinol.

NADH + acceptor = NAD(+) + reduced acceptor.

Cofactor

Binds 1 2Fe-2S cluster per subunit By similarity.

Binds 2 4Fe-4S clusters per subunit By similarity.

Subunit structure

Complex I is composed of about 45 different subunits By similarity.

Subcellular location

Mitochondrion inner membraneBy similarity. Note= Matrix and cytoplasmic side of the mitochondrial inner membrane By similarity.

Sequence similarities

Belongs to the complex I 75 kDa subunit family.

Contains 1 2Fe-2S ferredoxin-type domain.

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Ontologies

Keywords

   Biological processElectron transport
Respiratory chain
Transport
   Cellular componentMembrane
Mitochondrion
Mitochondrion inner membrane
   Ligand2Fe-2S
4Fe-4S
Iron
Iron-sulfur
Metal-binding
NAD
Ubiquinone
   Molecular functionOxidoreductase
   Technical termComplete proteome

Gene Ontology (GO)

None. [Check GOA]

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical view

Molecule processing

Chain1 – 688688NADH-ubiquinone oxidoreductase 75 kDa subunit

Regions

Domain1 – 85852Fe-2S ferredoxin-type

Sites

Metal binding381Iron-sulfur 1 (2Fe-2S) By similarity
Metal binding491Iron-sulfur 1 (2Fe-2S) By similarity
Metal binding521Iron-sulfur 1 (2Fe-2S) By similarity
Metal binding661Iron-sulfur 1 (2Fe-2S) By similarity
Metal binding1011Iron-sulfur 2 (4Fe-4S); via pros nitrogen By similarity
Metal binding1051Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding1081Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding1141Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding1531Iron-sulfur 3 (4Fe-4S) By similarity
Metal binding1561Iron-sulfur 3 (4Fe-4S) By similarity
Metal binding1591Iron-sulfur 3 (4Fe-4S) By similarity
Metal binding2041Iron-sulfur 3 (4Fe-4S) By similarity

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Sequences

Sequence LengthMass (Da)Tools
Q34312-1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 7091BF41C8A055F6

FASTA68879,838
        10         20         30         40         50         60 
MIIRFKINEI ECEVNEEKED ITILQACTAN GIEIPRFCYH EKLTIAGNCR MCLVYVTNEE 

        70         80         90        100        110        120 
KLLAACGIPL DENFDDESIE TEIDEILKAR EGVMEFLLIN HPLDCPICDQ GGECDLQEQT 

       130        140        150        160        170        180 
LAYGLDTGRF YIKKRAVEIK TFGRLIKGIM TRCIHCTRCV RFLTEIAGVN ELGVLGRGYN 

       190        200        210        220        230        240 
MEIGTYKKNV MIESELSGNI IDLCPVGALT SAVYAYKGRP WELKNIKGID IFDTLLTPIN 

       250        260        270        280        290        300 
YQVKGGEIFR ILPRINDRIN EEWITDKVRF HYESYKIIEK IRKETPSYKI QANKFIELTW 

       310        320        330        340        350        360 
KTALKMVFKV LLNKKNKVDL IIGSKINSTN LRIYKELMNR LGSKNYITEN GLMFKKFNYD 

       370        380        390        400        410        420 
LRENYINSND LYNVDKNDLV LLCGINLRVE SPLLNIKLRN VNFGDDEIES VKKIGIIGNK 

       430        440        450        460        470        480 
FDWKHESEYI GATLNSMLKL FEGRLPYCQQ IKKSKAPLII VGPSLLTRIS LTLQEMRAIF 

       490        500        510        520        530        540 
MKACNLKPEN ILIITQGANF GMALEEGLFK EKFSIGGNVL YSIDSNEVQV TNKINYVIYQ 

       550        560        570        580        590        600 
GIINDKFENK IDLYLPSKHY FEDFEGDREV YMNTFGQRSE IEKLSISKGN KIKENSMIGY 

       610        620        630        640        650        660 
IQLMYLNNKE MTRKEKEQKD IKLSYREMKK EEKRKIKVNK YLTINNIIEN YYMTDINIRL 

       670        680 
SKNLMITGQL RKEKKIMEAG IWKNRKCI

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References

[1]"The Dictyostelium discoideum mitochondrial genome: a primordial system using the universal code and encoding hydrophilic proteins atypical of metazoan mitochondrial DNA."
Cole R.A., Williams K.L.
J. Mol. Evol. 39:579-588(1994) [PubMed: 7807547] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: X22.
[2]"The mitochondrial DNA of Dictyostelium discoideum: complete sequence, gene content and genome organization."
Ogawa S., Yoshino R., Angata K., Iwamoto M., Pi M., Kuroe K., Matsuo K., Morio T., Urushihara H., Yanagisawa K., Tanaka Y.
Mol. Gen. Genet. 263:514-519(2000) [PubMed: 10821186] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: AX3.

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Cross-references

Sequence databases

U02291 Genomic DNA. Translation: AAA77667.1.
AB000109 Genomic DNA. Translation: BAA78086.1.
PIRT43783.
RefSeqNP_050104.1.

3D structure databases

ModBaseSearch...

Genome annotation databases

GeneID2193950.

Organism-specific databases

dictyBaseDDB0201618. nad11.

Family and domain databases

InterProIPR001041. Ferredoxin.
IPR006656. Mopterin_OxRdtase.
IPR000283. NADH_DHase_75KDa_su_CS.
IPR010228. NADH_quinone_OxRdtase_G.
[Graphical view]
PfamPF00111. Fer2. 1 hit.
PF00384. Molybdopterin. 1 hit.
[Graphical view]
TIGRFAMsTIGR01973. NuoG. 1 hit.
PROSITEPS00197. 2FE2S_FER_1. False negative.
PS51085. 2FE2S_FER_2. 1 hit.
PS00641. COMPLEX1_75K_1. 1 hit.
PS00642. COMPLEX1_75K_2. 1 hit.
PS00643. COMPLEX1_75K_3. 1 hit.
[Graphical view]
ProDomQ34312.
[Graphical view] [Entries sharing at least one domain]
BLOCKSSearch...

Other Resources

ProtoNetSearch...

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Entry information

Entry nameNDUS1_DICDI
AccessionPrimary (citable) accession number: Q34312
Entry history
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: November 1, 1996
Last modified: September 2, 2008
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

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Relevant documents

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents