Reviewed, UniProtKB/Swiss-Prot Q3T189 (DHSB_BOVIN)

Names and origin

Protein names

Recommended name:
    Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial
    EC=1.3.5.1
Alternative name(s):
    Iron-sulfur subunit of complex II
      Short name(s)=IP

Gene names

Name:

SDHB

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	280 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q) By similarity.
Catalytic activity	Succinate + ubiquinone = fumarate + ubiquinol.
Cofactor	Binds 1 2Fe-2S cluster By similarity. Binds 1 3Fe-4S cluster By similarity. Binds 1 4Fe-4S cluster By similarity.
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle.
Subunit structure	Component of complex II composed of four subunits: the flavoprotein (FP) SDHA, iron-sulfur protein (IP) SDHB, and a cytochrome b560 composed of SDHC and SDHD By similarity.
Subcellular location	Mitochondrion inner membrane; Peripheral membrane protein; Matrix sideBy similarity.
Sequence similarities	Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family. Contains 1 2Fe-2S ferredoxin-type domain. Contains 1 4Fe-4S ferredoxin-type domain.

Ontologies

Keywords
Biological process	Electron transport Transport Tricarboxylic acid cycle
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide
Ligand	2Fe-2S 3Fe-4S 4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Gene Ontology (GO)
Cellular component	mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	succinate dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Transit peptide	1 – 28	28	Mitochondrion By similarity
	Chain	29 – 280	252	Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial
Regions
	Domain	40 – 133	94	2Fe-2S ferredoxin-type
	Domain	176 – 206	31	4Fe-4S ferredoxin-type
Sites
	Metal binding	93	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	98	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	101	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	113	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	186	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	189	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	192	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	196	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	243	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	249	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	253	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Binding site	201	1	Ubiquinone; shared with DHSD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3T189-1 [UniParc]. Last modified October 11, 2005. Version 1. Checksum: BE51FF2193F8B6C5	FASTA	280	31,518
	10 20 30 40 50 60 MAAVVALSLR RRFPAAALGG ARLQACRGAQ TAAAAAPRIK KFAIYRWDPD KTGDKPHMQT 70 80 90 100 110 120 YEIDLNNCGP MVLDALIKIK NEIDSTLTFR RSCREGICGS CAMNINGGNT LACTRRIDTN 130 140 150 160 170 180 LSKVSKIYPL PHMYVIKDLV PDLSNFYAQY KSIEPYLKKK DESQGGKEQY LQSIEDREKL 190 200 210 220 230 240 DGLYECILCA CCSTSCPSYW WNGDKYLGPA VLMQAYRWMI DSRDDFTEER LAKLQDPFSL 250 260 270 280 YRCHTIMNCT QTCPKGLNPG KAIAEIKKMM ATYKEKQASA

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Ileum.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC102067 mRNA. Translation: AAI02068.1.
RefSeq	NP_001035573.1.
UniGene	Bt.13128
3D structure databases
SMR	Q3T189. Positions 37-275.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q3T189.
Genome annotation databases
Ensembl	ENSBTAG00000008314. Bos taurus. [Contig view]
GeneID	286840.
KEGG	bta:286840.
Phylogenomic databases
HOVERGEN	Q3T189.
Family and domain databases
InterPro	IPR006058. 2Fe2S_fd_BS. IPR001450. 4Fe4S_Fe_S_bd. IPR012675. b-grasp_ferredoxin-like. IPR001041. Ferredoxin. IPR012285. Fum_reductase_C. IPR004489. Succ_DHase/fum_Rdtase_Fe-S. [Graphical view]
Gene3D	G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit. G3DSA:1.10.1060.10. Fum_reductase_C. 1 hit.
Pfam	PF00111. Fer2. 1 hit. [Graphical view]
TIGRFAMs	TIGR00384. dhsB. 1 hit.
PROSITE	PS00197. 2FE2S_FER_1. 1 hit. PS51085. 2FE2S_FER_2. 1 hit. PS00198. 4FE4S_FER_1. 1 hit. PS51379. 4FE4S_FER_2. 1 hit. [Graphical view]
ProDom	Q3T189. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

DHSB_BOVIN

Accession

Primary (citable) accession number: Q3T189

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	October 11, 2005
Last modified:	July 22, 2008
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)