Reviewed, UniProtKB/Swiss-Prot Q3YSV8 (SYA_EHRCJ)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name(s)=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	Ecaj_0146

Organism

Ehrlichia canis (strain Jake) [Complete proteome] [HAMAP]

Taxonomic identifier

269484 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Anaplasmataceae › Ehrlichia

Protein attributes

Sequence length	887 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala).
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 887	887	Alanyl-tRNA synthetase

Sequences

	Sequence		Length	Mass (Da)
	Q3YSV8-1 [UniParc]. Last modified December 6, 2005. Version 2. Checksum: 28B4A4DFD83DFAF5	FASTA	887	99,906
	10 20 30 40 50 60 MKESNLVGDI RKLFVDFFIK NGHQLFPSSP LIVKDDPSLL FTNAGMVQFK HVFTDASNAN 70 80 90 100 110 120 VGTAVSSQKC LRVGGKHNDL ENVGYTNRHH TFFEMLGNFS FGDYFKEFAV ELAWSFVTKE 130 140 150 160 170 180 LALNKDKLYF TVYHEDQETF DLWKKISGFS ENRIIKIKTN DNFWSMGSTG PCGPCSEIFY 190 200 210 220 230 240 DYGEDIEGGL PGTPEEDGAR FTEIWNLVFM QYNRKSDGEL CALPKKCIDT GMGLERISAV 250 260 270 280 290 300 MQGVHDNYDI NLFKDLIKVS KKQSGNTNNE LAHRVIADHV RSAAFLIAEG LTPGNEGRDY 310 320 330 340 350 360 ILRRIIRRAA RYVYMLKYDG ALMYQIFPSL IDEKSYAYMA DYYPELINAK DLIISILKIE 370 380 390 400 410 420 EENFKDTLVK ALPLLEKELV GLSSGGVLPG DIAFKLYDTY GFPVDITLDI IKEKGIKFDE 430 440 450 460 470 480 QGFYDQMDKQ KERSKLNHSI KSVQQLKGKL WVDIKEHYGG TKFVGYEQYS TRAKVLSIIY 490 500 510 520 530 540 EGDKSTEVAN VGDRVNVLLD ITPFYAESGG QKGDTGVFNV IVRQGKELLS CDDVVEVLDT 550 560 570 580 590 600 KKVLDTLYIH ECVIKTGSLI VGDIICAEVN CEKRKNLCAN HSATHLLHYV LKSVIDRSII 610 620 630 640 650 660 QKGSLVSDDK LRFDFSYGVA LTKEQLTLIE DKMFSLIRNN SPVVTHICDL KEAIADGAVA 670 680 690 700 710 720 LFTEKYEDHG VRVINIGDSK ELCCGTHVRY TGEIGCFKIV SEASVACGVR RIEAVTGQHA 730 740 750 760 770 780 IDYFREQEKM LHLIAESVKS PIDNILIQID KLNRNNQELK QKLSDAYFSV INLQGIATDK 790 800 810 820 830 840 IGSIDFLYSS LNSVPIDVVR KFINEHLVND MIMFFSNVVG RNVMYVIGVA SNLHSKIKAT 850 860 870 880 DFVEIIGEVI KSKGGGNNQL AQISGEYIKE VDVIFHVKNK LINVLRN

References

[1]

"The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies." Mavromatis K., Doyle C.K., Lykidis A., Ivanova N., Francino M.P., Chain P., Shin M., Malfatti S., Larimer F., Copeland A., Detter J.C., Land M., Richardson P.M., Yu X.J., Walker D.H., McBride J.W., Kyrpides N.C. J. Bacteriol. 188:4015-4023(2006) [PubMed: 16707693] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000107 Genomic DNA. Translation: AAZ68197.1. Different initiation.
RefSeq	YP_302795.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3618289.
GenomeReviews	Gene locus Ecaj_0146 in contig CP000107_GR.
KEGG	ecn:Ecaj_0146.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q3YSV8.
Enzyme and pathway databases
BioCyc	ECAN269484:ECAJ_0146-MON.
Family and domain databases
HAMAP	MF_00036. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProDom	Q3YSV8. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

SYA_EHRCJ

Accession

Primary (citable) accession number: Q3YSV8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	December 6, 2005
Last modified:	July 22, 2008
This is version 22 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)