Reviewed, UniProtKB/Swiss-Prot Q3ZAE4 (SYA_DEHE1)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name(s)=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	DET0050

Organism

Dehalococcoides ethenogenes (strain 195) [Complete proteome] [HAMAP]

Taxonomic identifier

243164 [NCBI]

Taxonomic lineage

Bacteria › Chloroflexi › Dehalococcoidetes › Dehalococcoides

Protein attributes

Sequence length	871 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala).
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 871	871	Alanyl-tRNA synthetase

Sequences

	Sequence		Length	Mass (Da)
	Q3ZAE4-1 [UniParc]. Last modified September 27, 2005. Version 1. Checksum: 8E2AC4FEBD569CCF	FASTA	871	96,381
	10 20 30 40 50 60 MFSSDELREN YLKFFEEKGH KRIASSSLIP HNDPTLLLTT AGMVQFKPYY LGVAKPENSR 70 80 90 100 110 120 MTSCQKCFRT TDIESVGDAS HLTMFEMLGN FSIGNYFKKE AIAWAWEYVT QRLNIPAEKL 130 140 150 160 170 180 WVTVYLDDDE AIALWKEQGV PEGRIVRLGA ADNFWGPAGD SGPCGPCSEI HYDFGQEMGC 190 200 210 220 230 240 GKADCNPSCK CGRFCEIWNL VFVQFNQDKS GKRQNLPAPS IDTGMGLERL TILMQFRKNV 250 260 270 280 290 300 YETDIFAPVI EKACQLSGRQ YGIDAETDKA LRIVSEHSRG ITFLIADGVI PDKAGRGYVL 310 320 330 340 350 360 RRLLRRAVLF GRRLGLEKPF LVDMAGAVIA RMSGIYPELN KRQAYVLEMI ASEEARFSET 370 380 390 400 410 420 LATGLELLEE IVRQTKGGQI SGQDAFKLYD TYGFPVEMTT EIAAERGLSV DLGGFEAEME 430 440 450 460 470 480 VQRTKARSSR KFSFDAAATA EAVKNMRHGE KTCFVGYELT RQKSTIMDIL TEGGSVDSIE 490 500 510 520 530 540 EGDEASIVLD ESPFYAEMGG QVGDTGEIIT GGGRFEVKNT LHLPNGVFLH QGRVISGCLK 550 560 570 580 590 600 IAETAAAHID EERRRDIARN HTATHILQTA LRQVLGEQVQ QRGSVVTPER LRFDFSHLKP 610 620 630 640 650 660 MTKDEIRRAE EFVNDKIRRN LPVYAEEMPY RHALEEGVTA LFGEKYGDRV RVLRVGRPAV 670 680 690 700 710 720 SAELCGGTHV SASGEISLFK IVSESSVGAG LRRIEAVTGR EAEAYINLQQ DSLSELSGML 730 740 750 760 770 780 EAAPEESPRK LAELKEEIDT LKKTVQNLER QMSRGEAEEL LSKAEDYKGI KLLVSRMTSV 790 800 810 820 830 840 NADTLHETAD FLRDKLGSGI IVLGTVSEDK PFFLCMVTPD LIEKGYHAGN IVKKLSQIAG 850 860 870 GGGGGKPNMA QGGGRDKAKL DEALQAVKGM L

References

[1]

"Genome sequence of the PCE-dechlorinating bacterium Dehalococcoides ethenogenes." Seshadri R., Adrian L., Fouts D.E., Eisen J.A., Phillippy A.M., Methe B.A., Ward N.L., Nelson W.C., DeBoy R.T., Khouri H.M., Kolonay J.F., Dodson R.J., Daugherty S.C., Brinkac L.M., Sullivan S.A., Madupu R., Nelson K.E., Kang K.H. , Impraim M., Tran K., Robinson J.M., Forberger H.A., Fraser C.M., Zinder S.H., Heidelberg J.F. Science 307:105-108(2005) [PubMed: 15637277] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000027 Genomic DNA. Translation: AAW39097.1.
RefSeq	YP_180805.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3229054.
GenomeReviews	Gene locus DET0050 in contig CP000027_GR.
KEGG	det:DET0050.
NMPDR	fig|243164.3.peg.62.
TIGR	DET0050.
Phylogenomic databases
HOGENOM	Q3ZAE4.
Enzyme and pathway databases
BioCyc	DETH243164:DET_0050-MON.
Family and domain databases
HAMAP	MF_00036. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR003156. Pesterase_DHHA1. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProDom	Q3ZAE4. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

SYA_DEHE1

Accession

Primary (citable) accession number: Q3ZAE4

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	September 27, 2005
Last modified:	July 22, 2008
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)