Reviewed, UniProtKB/Swiss-Prot Q40704 (MADS3_ORYSJ)

Names and origin

Protein names

Recommended name:
    MADS-box transcription factor 3
Alternative name(s):
    OsMADS3
    Protein AGAMOUS-like
    RMADS222

Gene names

Name:	MADS3
Synonyms:	RAG
Ordered Locus Names:	Os01g0201700, LOC_Os01g10504

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	236 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Probable transcription factor involved in the development of floral organs. Acts as C-class protein in association with MADS58. Involved in the control of lodicule number (whorl 2), stamen specification (whorl 3) and floral meristem determinacy (whorl 4), but not in the regulation of carpel morphogenesis. Plays a more predominant role in controlling lodicule development and in specifying stamen identity than MADS58.
Subcellular location	NucleusProbable.
Tissue specificity	Expressed in lemmas, paleas and lodicules.
Developmental stage	Expressed at early stage of flower development in stamen anlagen before stamen initiation, and at later stage in lodicule and ovule primordia initiation region.
Miscellaneous	Plants lacking MADS3 show homeotic transformation of stamens into lodicules, and ectopic development of lodicules where they do not form in the wild-type. The transformation of lodicules into stamens is observed in overexpressing plants.
Sequence similarities	Contains 1 K-box domain. Contains 1 MADS-box domain.

Ontologies

Keywords
Biological process	Differentiation Flowering Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Ligand	DNA-binding
Molecular function	Activator Developmental protein
Gene Ontology (GO)
None. [Check GOA]

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q40704-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q40704-2)

The sequence of this isoform differs from the canonical sequence as follows:
     231-236: QQPAFN → SRPSISFGVDTVRTHVR

Notes: No experimental confirmation available.

Isoform 3 (identifier: Q40704-3)

The sequence of this isoform differs from the canonical sequence as follows:
     110-114: RTIVG → LTRLA
     115-236: Missing.

Notes: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 236	236	MADS-box transcription factor 3
Regions
	Domain	1 – 61	61	MADS-box
	Domain	87 – 178	92	K-box
Natural variations
	Alternative sequence	110 – 114	5	RTIVG → LTRLA in isoform 3.
	Alternative sequence	115 – 236	122	Missing in isoform 3.
	Alternative sequence	231 – 236	6	QQPAFN → SRPSISFGVDTVRTHVR in isoform 2.

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 241BD9BCE2DAD35F	FASTA	236	27,141
	10 20 30 40 50 60 MGRGKIEIKR IENTTNRQVT FCKRRNGLLK KAYELSVLCD AEVALIVFSS RGRLYEYANN 70 80 90 100 110 120 SVKSTVERYK KANSDTSNSG TVAEVNAQHY QQESSKLRQQ ISSLQNANSR TIVGDSINTM 130 140 150 160 170 180 SLRDLKQVEN RLEKGIAKIR ARKNELLYAE VEYMQKREVE LQNDNMYLRS KVVENERGQQ 190 200 210 220 230 PLNMMGAAST SEYDHMVNNP YDSRNFLQVN IMQQPQHYAH QLQPTTLQLG QQPAFN
	Isoform 2 [UniParc]. Checksum: 893BFAE64504C568		247	28,351
	10 20 30 40 50 60 MGRGKIEIKR IENTTNRQVT FCKRRNGLLK KAYELSVLCD AEVALIVFSS RGRLYEYANN 70 80 90 100 110 120 SVKSTVERYK KANSDTSNSG TVAEVNAQHY QQESSKLRQQ ISSLQNANSR TIVGDSINTM 130 140 150 160 170 180 SLRDLKQVEN RLEKGIAKIR ARKNELLYAE VEYMQKREVE LQNDNMYLRS KVVENERGQQ 190 200 210 220 230 240 PLNMMGAAST SEYDHMVNNP YDSRNFLQVN IMQQPQHYAH QLQPTTLQLG SRPSISFGVD TVRTHVR
	Isoform 3 [UniParc]. Checksum: 8B935087C09E5B69		114	12,875
	10 20 30 40 50 60 MGRGKIEIKR IENTTNRQVT FCKRRNGLLK KAYELSVLCD AEVALIVFSS RGRLYEYANN 70 80 90 100 110 SVKSTVERYK KANSDTSNSG TVAEVNAQHY QQESSKLRQQ ISSLQNANSL TRLA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Phenotypic alterations of petal and sepal by ectopic expression of a rice MADS box gene in tobacco." Kang H.-G., Noh Y.-S., Chung Y.-Y., Costa M.A., An K., An G. Plant Mol. Biol. 29:1-10(1995) [PubMed: 7579155] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Flower.
[2]	"Molecular and phylogenetic analyses of the complete MADS-box transcription factor family in rice." Yao Q., Peng R., Xiong A. Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[3]	"The genome sequence and structure of rice chromosome 1." Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T. Nature 420:312-316(2002) [PubMed: 12447438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[4]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Strain: cv. Nipponbare.
[5]	"Identification of class B and class C floral organ identity genes from rice plants." Kang H.-G., Jeon J.-S., Lee S., An G. Plant Mol. Biol. 38:1021-1029(1998) [PubMed: 9869408] [Abstract] Cited for: FUNCTION.
[6]	"Spatially and temporally regulated expression of rice MADS box genes with similarity to Arabidopsis class A, B and C genes." Kyozuka J., Kobayashi T., Morita M., Shimamoto K. Plant Cell Physiol. 41:710-718(2000) [PubMed: 10945340] [Abstract] Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[7]	"Ectopic expression of OsMADS3, a rice ortholog of AGAMOUS, caused a homeotic transformation of lodicules to stamens in transgenic rice plants." Kyozuka J., Shimamoto K. Plant Cell Physiol. 43:130-135(2002) [PubMed: 11828031] [Abstract] Cited for: FUNCTION.
[8]	"Functional diversification of the two C-class MADS box genes OSMADS3 and OSMADS58 in Oryza sativa." Yamaguchi T., Lee D.-Y., Miyao A., Hirochika H., An G., Hirano H. Plant Cell 18:15-28(2006) [PubMed: 16326928] [Abstract] Cited for: FUNCTION, DEVELOPMENTAL STAGE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L37528 mRNA. Translation: AAA99964.1. AY553724 mRNA. Translation: AAS60204.1. AP003105 Genomic DNA. No translation available. AK108568 mRNA. No translation available.
PIR	S59480.
3D structure databases
HSSP	HSSP built from PDB template 1EGW based on UniProtKB Q02078.
ModBase	Search...
Organism-specific databases
Gramene	Q40704.
Family and domain databases
InterPro	IPR002487. TF_Kbox. IPR002100. TF_MADSbox. [Graphical view]
Pfam	PF01486. K-box. 1 hit. PF00319. SRF-TF. 1 hit. [Graphical view]
PRINTS	PR00404. MADSDOMAIN.
SMART	SM00432. MADS. 1 hit. [Graphical view]
PROSITE	PS51297. K_BOX. 1 hit. PS00350. MADS_BOX_1. 1 hit. PS50066. MADS_BOX_2. 1 hit. [Graphical view]
ProDom	Q40704. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

MADS3_ORYSJ

Accession

Primary (citable) accession number: Q40704
Secondary accession number(s): Q6Q7W1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	November 1, 1996
Last modified:	July 22, 2008
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)