Reviewed, UniProtKB/Swiss-Prot Q44257 (CBAB_COMTE)

Names and origin

Protein names

Recommended name:
    3-chlorobenzoate-3,4-dioxygenase reductase subunit
    EC=1.14.-.-

Gene names

Name:

cbaB

Organism

Comamonas testosteroni (Pseudomonas testosteroni)

Taxonomic identifier

285 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Comamonas

Protein attributes

Sequence length	315 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Cofactor	FMN By similarity.
Subunit structure	This dioxygenase system consists of two proteins: phthalate oxygenase and phthalate oxygenase reductase.
Sequence similarities	Belongs to the PDR/vanB family. Contains 1 2Fe-2S ferredoxin-type domain. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism Electron transport Transport
Ligand	2Fe-2S FMN Flavoprotein Iron Iron-sulfur Metal-binding NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 315	315	3-chlorobenzoate-3,4-dioxygenase reductase subunit
Regions
	Domain	7 – 109	103	FAD-binding FR-type
	Domain	228 – 315	88	2Fe-2S ferredoxin-type
	Nucleotide binding	1 – 103	103	FMN By similarity
Sites
	Metal binding	264	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	269	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	272	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	302	1	Iron-sulfur (2Fe-2S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q44257-1 [UniParc]. Last modified May 27, 2002. Version 2. Checksum: B88E16DA94A8E7A3	FASTA	315	34,669
	10 20 30 40 50 60 MVAIDQHDTY SVRVISRSHL SKDIVQVELE ESSQRPLPDY EPGSHVDIYV QDDLVRQYSL 70 80 90 100 110 120 VKASDAQASY QIAFKVKRDQ GSATELMCEL LRVGATTRIS APRNAFALDP QARETVLICG 130 140 150 160 170 180 GIGITPMVHM AMTLVKAKRP WSMHIASRDG DELDLLGPLG SCSEISRYIS SQGDRLPIRD 190 200 210 220 230 240 LAERAPANAH LYFCGPEGML QEFLAATQHR DPATVHYEQF QAAPSTGNEF TVNLARSGAQ 250 260 270 280 290 300 YVVREGETIL DVLRNAGHHV TSSCRQGICG MCETTLISGV PDHRDRLLTD SEKASGRTML 310 ICCSRALSPE LTLDL

References

[1]	"The nucleotide sequence of the Tn5271 3-chlorobenzoate 3,4-dioxygenase genes (cbaAB) unites the class IA oxygenases in a single lineage." Nakatsu C.H., Straus N.A., Wyndham R.C. Microbiology 141:485-495(1995) [PubMed: 7704279] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: BR60 / Isolate Bloody Run creek. Transposon: Tn5271.
[2]	Wyndham R.C. Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U18133 Genomic DNA. Translation: AAC45717.2.
3D structure databases
HSSP	HSSP built from PDB template 2PIA based on UniProtKB P33164.
ModBase	Search...
Family and domain databases
InterPro	IPR006058. 2Fe2S_fd_BS. IPR012675. b-grasp_ferredoxin-like. IPR001041. Ferredoxin. IPR001709. FPN_cyt_redctse. IPR008333. OxRdtase_FAD-bd. IPR001433. OxRdtase_FAD/NAD_bd. IPR000951. Ph_dOase_redase_FPNCR. [Graphical view]
Gene3D	G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit.
Pfam	PF00970. FAD_binding_6. 1 hit. PF00111. Fer2. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PRINTS	PR00371. FPNCR. PR00409. PHDIOXRDTASE.
PROSITE	PS00197. 2FE2S_FER_1. 1 hit. PS51085. 2FE2S_FER_2. 1 hit. PS51384. FAD_FR. 1 hit. [Graphical view]
ProDom	Q44257. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

CBAB_COMTE

Accession

Primary (citable) accession number: Q44257
Secondary accession number(s): O08106

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	May 27, 2002
Last modified:	July 22, 2008
This is version 53 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)