Reviewed, UniProtKB/Swiss-Prot Q44633 (MNME_BUCAP)

Names and origin

Protein names

Recommended name:
    tRNA modification GTPase mnmE
    EC=3.6.-.-

Gene names

Name:	mnmE
Synonyms:	thdF, trmE
Ordered Locus Names:	BUsg_017

Organism

Buchnera aphidicola subsp. Schizaphis graminum [Complete proteome] [HAMAP]

Taxonomic identifier

98794 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	456 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34 By similarity.
Cofactor	Binds 1 potassium ion per subunit By similarity.
Subunit structure	Homodimer By similarity. Heterotetramer of two mnmE and two mnmG subunits By similarity.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the era/mnmE GTP-binding protein family. MnmE subfamily.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	GTP-binding Magnesium Metal-binding Nucleotide-binding Potassium
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA modification Inferred from electronic annotation. Source: HAMAP
Molecular function	GTP binding Inferred from electronic annotation. Source: HAMAP GTPase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 456	456	tRNA modification GTPase mnmE
Regions
	Nucleotide binding	228 – 233	6	GTP By similarity
	Nucleotide binding	247 – 253	7	GTP By similarity
	Nucleotide binding	272 – 275	4	GTP By similarity
Sites
	Metal binding	228	1	Potassium By similarity
	Metal binding	232	1	Magnesium By similarity
	Metal binding	247	1	Potassium; via carbonyl oxygen By similarity
	Metal binding	249	1	Potassium; via carbonyl oxygen By similarity
	Metal binding	252	1	Potassium By similarity
	Metal binding	253	1	Magnesium By similarity
	Binding site	23	1	Formyltetrahydrofolate By similarity
	Binding site	80	1	Formyltetrahydrofolate By similarity
	Binding site	122	1	Formyltetrahydrofolate By similarity
	Binding site	456	1	Formyltetrahydrofolate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q44633-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: FC5BE94CB646DEBA	FASTA	456	51,695
	10 20 30 40 50 60 MIRNDTIIAQ VTCPGKSAVG ILRVSGIHAN QVAFAVLGKI PKPRFATYSK FFDESKKVLD 70 80 90 100 110 120 EGISLWFPAP FSLTGEDVLE LQGHGNPFIM DLLIKRILCL KNIKIRIAQP GEFCQRAFLN 130 140 150 160 170 180 GKIDLIQAEA IDDLINSETE SVVRASLNSL HGNFSFYIQK IIKKLIEFRT NIEASIDFSE 190 200 210 220 230 240 ENIDFDFNIF IMSNFEKLND KFLKIKNIVS EGSLIREAKR IVIVGPPNAG KSSLLNVLSC 250 260 270 280 290 300 RDRAIVTDLP GTTRDVLYEN INIHGISCEI IDTAGLRETE DKIEKIGIQR SWEMIKNSDH 310 320 330 340 350 360 VLYVMDKTIS LEDQKKTSIQ FMKQISSYNI QEVTFVLNKN DLVEDFCGIT KIENLLFISI 370 380 390 400 410 420 SALTGQGIDI LKKHLSNRQK DKSQEGLFIA RRRHIHQIDL SYCELLKAQK NWLKYKNIEL 430 440 450 LAESLNIINK LLGEITGEFT SSDLLKRIFS TFCIGK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of 60K, tdhF, groES and groEL genes of Buchnera aphidicola." Anwarul H.K., Moriya S., Baumann P., Yoshikawa H., Ogasawara N. Submitted (JUN-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence analysis of a 34.7-kb DNA segment from the genome of Buchnera aphidicola (endosymbiont of aphids) containing groEL, dnaA, the atp operon, gidA, and rho." Clark M.A., Baumann L., Baumann P. Curr. Microbiol. 36:158-163(1998) [PubMed: 9516544] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed: 12089438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	D85628 Genomic DNA. Translation: BAA12845.1. AF008210 Genomic DNA. Translation: AAC38101.1. AE013218 Genomic DNA. Translation: AAM67589.1.
RefSeq	NP_660378.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1005833.
GenomeReviews	Gene locus BUsg_017 in contig AE013218_GR.
KEGG	bas:BUsg017.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q44633.
Enzyme and pathway databases
BioCyc	BAPH198804:BUSG017-MON.
Family and domain databases
HAMAP	MF_00379. [Tree]
InterPro	IPR005289. GTP_bd. IPR002917. MMR_HSR1_GTP_bd. IPR005225. Small_GTP_bd. IPR004520. ThdF. [Graphical view]
Pfam	PF01926. MMR_HSR1. 1 hit. [Graphical view]
TIGRFAMs	TIGR00650. MG442. 1 hit. TIGR00450. mnmE_trmE_thdF. 1 hit. TIGR00231. small_GTP. 1 hit.
ProDom	Q44633. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

MNME_BUCAP

Accession

Primary (citable) accession number: Q44633

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	September 2, 2008
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)