Reviewed, UniProtKB/Swiss-Prot Q45489 (MTB2_BACSU)

Names and origin

Protein names

Recommended name:
    Modification methylase BglII
      Short name=M.BglII
    EC=2.1.1.113
Alternative name(s):
    N(4)- cytosine-specific methyltransferase BglII

Gene names

Name:

bglIIM

Organism

Bacillus subtilis

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	360 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence AGATCT, causes specific methylation on C-5 on both strands, and protects the DNA from cleavage by the BglII endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA cytosine = S-adenosyl-L-homocysteine + DNA N(4)-methylcytosine.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 360	360	Modification methylase BglII

Sequences

	Sequence		Length	Mass (Da)
	Q45489-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: B1C61DF49E5285D5	FASTA	360	42,043
	10 20 30 40 50 60 MSEDQYKQIK LHLGMEDDNE DLPNHIPSSF PKQHLNKIYN GDTMNMLLDI PDNSVDLVVT 70 80 90 100 110 120 SPPYNINKFK NDRRPLEEYL KWQTEIIEQC HRVLKPSGSI FWQVGTYVND SGAHIPLDIR 130 140 150 160 170 180 FFPIFESLGM FPRNRIVWVR PHGLHANKKF AGRHETILWF TKTPEYKFFL DPIRVPQKYA 190 200 210 220 230 240 NKKHYKGDKK GELSGDPLGK NPGDVWAFRN VRHNHEEDTI HPTQYPEDMI ERIVLSTTEP 250 260 270 280 290 300 NDIVLDPFIG MGTTASVAKN LNRYFYGAEI EKEYVDIAYQ ILSGEPDENN NFPNLKTLRQ 310 320 330 340 350 360 YCEKNGIIDP SQYTFTRQRK GSKPSLDSKA HPEEHHKKEI VERIEFEAEN SVYKKVQNEQ

References

[1]

"Cloning and characterization of the BglII restriction-modification system reveals a possible evolutionary footprint." Anton B.P., Heiter D.F., Benner J.S., Hess E.J., Greenough L., Moran L.S., Slatko B.E., Brooks J.E. Gene 187:19-27(1997) [PubMed: 9073062] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Globigii / RUB562.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U49842 Genomic DNA. Translation: AAC45061.1.
PIR	JC6322.
3D structure databases
HSSP	HSSP built from PDB template 1G60 based on UniProtKB P23192.
ModBase	Search...
Protein family/group databases
REBASE	3304. M.BglII.
Family and domain databases
InterPro	IPR002295. D21N6_MeTrfase. IPR001091. MeTrfase_CN4. IPR002941. N6/N4_Mtase. [Graphical view]
Pfam	PF01555. N6_N4_Mtase. 1 hit. [Graphical view]
PRINTS	PR00506. D21N6MTFRASE. PR00508. S21N4MTFRASE.
PROSITE	PS00093. N4_MTASE. 1 hit. [Graphical view]
ProDom	Q45489. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

MTB2_BACSU

Accession

Primary (citable) accession number: Q45489

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 16, 2002
Last sequence update:	November 1, 1996
Last modified:	September 2, 2008
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)