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Q45614

- YYCG_BACSU

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Protein
Sensor histidine kinase YycG
Gene
yycG, BSU40400

Gene:

yycG, BSU40400
Organism
Bacillus subtilis (strain 168)
Status
Reviewed - - Experimental evidence at protein leveli

UniProt

Q45614 - YYCG_BACSU

Protein:

Sensor histidine kinase YycG
Protein Existence: Experimental evidence at protein level

Functioni

Member of the two-component regulatory system YycG/YycF involved in the regulation of the ftsAZ operon, the yocH and ykvT, cwlO, lytE, ydjM, yjeA, yoeB genes and the tagAB and tagDEF operons. Probably phosphorylates YycF.3 Publications

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

GO - Molecular functioni

  1. ATP bindingInferred from electronic annotationi Source: UniProtKB-KW
  2. phosphorelay sensor kinase activityInferred from electronic annotationi Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. ATP bindingInferred from electronic annotationi Source: UniProtKB-KW
  2. phosphorelay sensor kinase activityInferred from electronic annotationi Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Two-component regulatory system

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU40400-MONOMER.
BRENDAi2.7.13.3. 700.

Names & Taxonomyi

Protein namesi
Recommended name:
Sensor histidine kinase YycG (EC:2.7.13.3)
Gene namesi
Name:yycG
Ordered Locus Names:BSU40400
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570: Chromosome

Organism-specific databases

GenoListiBSU40400.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier
Topological domaini1 – 1313Cytoplasmic Reviewed prediction
Transmembranei14 – 3421Helical; Reviewed prediction
Topological domaini35 – 182148Extracellular Reviewed prediction
Transmembranei183 – 20321Helical; Reviewed prediction
Topological domaini204 – 611408Cytoplasmic Reviewed prediction

GO - Cellular componenti

  1. integral component of membraneInferred from electronic annotationi Source: UniProtKB-KW
  2. plasma membraneInferred from electronic annotationi Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier
Mutagenesisi3861H → R or A: Loss of phosphorylation. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier
Chaini1 – 611611Sensor histidine kinase YycG
PRO_0000074918

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier
Modified residuei3861Phosphohistidine; by autocatalysis Inferred

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ45614.

Expressioni

Developmental stagei

Expressed during exponential growth and shut down at the entry into stationary phase.

Interactioni

Subunit structurei

Homodimer. Interacts with YycH and YycI.1 Publication

Protein-protein interaction databases

IntActiQ45614. 2 interactions.
STRINGi224308.BSU40400.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier
Beta strandi451 – 4533
Helixi454 – 46613
Beta strandi475 – 4784
Beta strandi485 – 4884
Helixi490 – 50617
Beta strandi514 – 5218
Turni522 – 5254
Beta strandi526 – 5327
Turni540 – 5478
Helixi567 – 57711
Beta strandi582 – 5876
Turni588 – 5903
Beta strandi591 – 60111

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3SL2X-ray1.61A451-611[»]
ProteinModelPortaliQ45614.
SMRiQ45614. Positions 203-255, 264-604.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier
Domaini204 – 25653HAMP
Domaini263 – 32462PAS
Domaini325 – 37955PAC
Domaini383 – 602220Histidine kinase

Sequence similaritiesi

Contains 1 HAMP domain.

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5002.
HOGENOMiHOG000223176.
KOiK07652.
OMAiMQVIDNI.
OrthoDBiEOG6G4VQG.
ProtClustDBiCLSK888192.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR003661. EnvZ-like_dim/P.
IPR003660. HAMP_linker_domain.
IPR003594. HATPase_ATP-bd.
IPR001610. PAC.
IPR000014. PAS.
IPR000700. PAS-assoc_C.
IPR004358. Sig_transdc_His_kin-like_C.
IPR005467. Sig_transdc_His_kinase_core.
IPR009082. Sig_transdc_His_kinase_dimeric.
[Graphical view]
PfamiPF00672. HAMP. 1 hit.
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF13426. PAS_9. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00304. HAMP. 1 hit.
SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00086. PAC. 1 hit.
SM00091. PAS. 1 hit.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF55785. SSF55785. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiPS50885. HAMP. 1 hit.
PS50109. HIS_KIN. 1 hit.
PS50113. PAC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q45614-1 [UniParc]FASTA

« Hide

MNKVGFFRSI QFKITLIYVL LIIIAMQIIG VYFVNQVEKS LISSYEQSLN    50
QRIDNLSYYI EQEYKSDNDS TVIKDDVSRI LNDFTKSDEV REISFVDKSY 100
EVVGSSKPYG EEVAGKQTTD LIFKRIFSTK QSYLRKYYDP KSKIRVLISA 150
KPVMTENQEV VGAIYVVASM EDVFNQMKTI NTILASGTGL ALVLTALLGI 200
FLARTITHPL SDMRKQAMEL AKGNFSRKVK KYGHDEIGQL ATTFNHLTRE 250
LEDAQAMTEG ERRKLASVIA YMTDGVIATN RNGAIILLNS PALELLNVSR 300
ETALEMPITS LLGLQENYTF EDLVEQQDSM LLEIERDDEL TVLRVNFSVI 350
QREHGKIDGL IAVIYDVTEQ EKMDQERREF VANVSHELRT PLTTMRSYLE 400
ALAEGAWENK DIAPRFLMVT QNETERMIRL VNDLLQLSKF DSKDYQFNRE 450
WIQIVRFMSL IIDRFEMTKE QHVEFIRNLP DRDLYVEIDQ DKITQVLDNI 500
ISNALKYSPE GGHVTFSIDV NEEEELLYIS VKDEGIGIPK KDVEKVFDRF 550
YRVDKARTRK LGGTGLGLAI AKEMVQAHGG DIWADSIEGK GTTITFTLPY 600
KEEQEDDWDE A 611
Length:611
Mass (Da):70,034
Last modified:November 1, 1996 - v1
Checksum:i2E7B7CB538410AE7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D78193 Genomic DNA. Translation: BAA11299.1.
AL009126 Genomic DNA. Translation: CAB16077.1.
PIRiF70089.
RefSeqiNP_391920.1. NC_000964.3.

Genome annotation databases

EnsemblBacteriaiCAB16077; CAB16077; BSU40400.
GeneIDi937793.
KEGGibsu:BSU40400.
PATRICi18980158. VBIBacSub10457_4241.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D78193 Genomic DNA. Translation: BAA11299.1 .
AL009126 Genomic DNA. Translation: CAB16077.1 .
PIRi F70089.
RefSeqi NP_391920.1. NC_000964.3.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3SL2 X-ray 1.61 A 451-611 [» ]
ProteinModelPortali Q45614.
SMRi Q45614. Positions 203-255, 264-604.
ModBasei Search...

Protein-protein interaction databases

IntActi Q45614. 2 interactions.
STRINGi 224308.BSU40400.

Chemistry

ChEMBLi CHEMBL1075059.

Proteomic databases

PaxDbi Q45614.

Protocols and materials databases

DNASUi 937793.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAB16077 ; CAB16077 ; BSU40400 .
GeneIDi 937793.
KEGGi bsu:BSU40400.
PATRICi 18980158. VBIBacSub10457_4241.

Organism-specific databases

GenoListi BSU40400.

Phylogenomic databases

eggNOGi COG5002.
HOGENOMi HOG000223176.
KOi K07652.
OMAi MQVIDNI.
OrthoDBi EOG6G4VQG.
ProtClustDBi CLSK888192.

Enzyme and pathway databases

BioCyci BSUB:BSU40400-MONOMER.
BRENDAi 2.7.13.3. 700.

Family and domain databases

Gene3Di 1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
InterProi IPR003661. EnvZ-like_dim/P.
IPR003660. HAMP_linker_domain.
IPR003594. HATPase_ATP-bd.
IPR001610. PAC.
IPR000014. PAS.
IPR000700. PAS-assoc_C.
IPR004358. Sig_transdc_His_kin-like_C.
IPR005467. Sig_transdc_His_kinase_core.
IPR009082. Sig_transdc_His_kinase_dimeric.
[Graphical view ]
Pfami PF00672. HAMP. 1 hit.
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF13426. PAS_9. 1 hit.
[Graphical view ]
PRINTSi PR00344. BCTRLSENSOR.
SMARTi SM00304. HAMP. 1 hit.
SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00086. PAC. 1 hit.
SM00091. PAS. 1 hit.
[Graphical view ]
SUPFAMi SSF47384. SSF47384. 1 hit.
SSF55785. SSF55785. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEi PS50885. HAMP. 1 hit.
PS50109. HIS_KIN. 1 hit.
PS50113. PAC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publicationsDownload
  1. "Sequence analysis of the 36-kb region between gntZ and trnY genes of Bacillus subtilis genome."
    Kasahara Y., Nakai S., Ogasawara N.
    DNA Res. 4:155-159(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  3. "A two-component signal transduction system essential for growth of Bacillus subtilis: implications for anti-infective therapy."
    Fabret C., Hoch J.A.
    J. Bacteriol. 180:6375-6383(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Strain: 168 / JH642.
  4. "The essential two-component regulatory system encoded by yycF and yycG modulates expression of the ftsAZ operon in Bacillus subtilis."
    Fukuchi K., Kasahara Y., Asai K., Kobayashi K., Moriya S., Ogasawara N.
    Microbiology 146:1573-1583(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Strain: 168.
  5. "Antibacterial agents that inhibit histidine protein kinase YycG of Bacillus subtilis."
    Yamamoto K., Kitayama T., Minagawa S., Watanabe T., Sawada S., Okamoto T., Utsumi R.
    Biosci. Biotechnol. Biochem. 65:2306-2310(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF HIS-386, INHIBITION BY IMIDAZOLE AND ZERUMBONE DERIVATIVES.
  6. "Genes controlled by the essential YycG/YycF two-component system of Bacillus subtilis revealed through a novel hybrid regulator approach."
    Howell A., Dubrac S., Andersen K.K., Noone D., Fert J., Msadek T., Devine K.
    Mol. Microbiol. 49:1639-1655(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: STUDY OF THE YYCF/YYCG REGULON.
  7. "YycH and YycI interact to regulate the essential YycFG two-component system in Bacillus subtilis."
    Szurmant H., Mohan M.A., Imus P.M., Hoch J.A.
    J. Bacteriol. 189:3280-3289(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH YYCH AND YYCI, SUBUNIT.
  8. "The essential YycFG two-component system controls cell wall metabolism in Bacillus subtilis."
    Bisicchia P., Noone D., Lioliou E., Howell A., Quigley S., Jensen T., Jarmer H., Devine K.M.
    Mol. Microbiol. 65:180-200(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry nameiYYCG_BACSU
AccessioniPrimary (citable) accession number: Q45614
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The imidazole derivatives NH125, NH126 and NH127 inhibited the incorporation of phosphate from ATP at 50 µg/ml. The zerumbone derivative NH0891 inhibited YycG.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

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