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Reviewed, UniProtKB/Swiss-Prot Q46856 (YQHD_ECOLI)

Last modified September 2, 2008. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Alcohol dehydrogenase yqhD
    EC=1.1.1.-
Gene names
Name: yqhD
Ordered Locus Names: b3011, JW2978
OrganismEscherichia coli (strain K12) [Complete proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

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Protein attributes

Sequence length387 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

NADP-dependent ADH activity.

Catalytic activity

An alcohol + NADP(+) = an aldehyde + NADPH.

Cofactor

Binds 1 zinc ion per subunit.

Subunit structure

Homodimer.

Sequence similarities

Belongs to the iron-containing alcohol dehydrogenase family.

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Ontologies

Keywords

   LigandMetal-binding
NADP
Zinc
   Molecular functionOxidoreductase
   Technical term3D-structure
Complete proteome

Gene Ontology (GO)

None. [Check GOA]

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical view

Molecule processing

Chain1 – 387387Alcohol dehydrogenase yqhD

Regions

Nucleotide binding38 – 403NADP
Nucleotide binding93 – 997NADP
Nucleotide binding179 – 1824NADP

Sites

Metal binding1941Zinc; catalytic
Metal binding1981Zinc; catalytic
Metal binding2671Zinc; catalytic
Metal binding2811Zinc; catalytic
Binding site1381NADP
Binding site1471NADP
Binding site1601NADP

Secondary structure

1 ............................................................. 387
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
Q46856-1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 448A7610787D2146

FASTA38742,097
        10         20         30         40         50         60 
MNNFNLHTPT RILFGKGAIA GLREQIPHDA RVLITYGGGS VKKTGVLDQV LDALKGMDVL 

        70         80         90        100        110        120 
EFGGIEPNPA YETLMNAVKL VREQKVTFLL AVGGGSVLDG TKFIAAAANY PENIDPWHIL 

       130        140        150        160        170        180 
QTGGKEIKSA IPMGCVLTLP ATGSESNAGA VISRKTTGDK QAFHSAHVQP VFAVLDPVYT 

       190        200        210        220        230        240 
YTLPPRQVAN GVVDAFVHTV EQYVTKPVDA KIQDRFAEGI LLTLIEDGPK ALKEPENYDV 

       250        260        270        280        290        300 
RANVMWAATQ ALNGLIGAGV PQDWATHMLG HELTAMHGLD HAQTLAIVLP ALWNEKRDTK 

       310        320        330        340        350        360 
RAKLLQYAER VWNITEGSDD ERIDAAIAAT RNFFEQLGVP THLSDYGLDG SSIPALLKKL 

       370        380 
EEHGMTQLGE NHDITLDVSR RIYEAAR

« Hide

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References

« Hide 'large scale' references
[1]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[2]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"Crystal structure of E.coli alcohol dehydrogenase YqhD: evidence of a covalently modified NADP coenzyme."
Sulzenbacher G., Alvarez K., Van Den Heuvel R.H., Versluis C., Spinelli S., Campanacci V., Valencia C., Cambillau C., Eklund H., Tegoni M.
J. Mol. Biol. 342:489-502(2004) [PubMed: 15327949] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 2-387 IN COMPLEX WITH ZINC IONS AND NADP, FUNCTION, SUBUNIT.

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Cross-references

Sequence databases

U28377 Genomic DNA. Translation: AAA69178.1.
U00096 Genomic DNA. Translation: AAC76047.1.
AP009048 Genomic DNA. Translation: BAE77068.1.
PIRA65088.
RefSeqAP_003562.1.
NP_417484.1.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1OJ7X-ray2.00A/B/C/D2-387[»]
ModBaseSearch...

Protein-protein interaction databases

IntActQ46856.

Genome annotation databases

GeneID947493.
GenomeReviewsGene locus b3011 in contig U00096_GR.
Gene locus JW2978 in contig AP009048_GR.
KEGGecj:JW2978.
eco:b3011.

Organism-specific databases

EchoBASEEB2834.
EcoGeneEG13014. yqhD.
CMRSearch...

Phylogenomic databases

HOGENOMQ46856.

Enzyme and pathway databases

BioCycEcoCyc:G7564-MON.
MetaCyc:G7564-MON.

Family and domain databases

InterProIPR001670. Fe_AlcDHase.
[Graphical view]
PfamPF00465. Fe-ADH. 1 hit.
[Graphical view]
PROSITEPS00913. ADH_IRON_1. 1 hit.
PS00060. ADH_IRON_2. 1 hit.
[Graphical view]
ProDomQ46856.
[Graphical view] [Entries sharing at least one domain]
BLOCKSSearch...

Other Resources

ProtoNetSearch...

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Entry information

Entry nameYQHD_ECOLI
AccessionPrimary (citable) accession number: Q46856
Secondary accession number(s): Q2M9I8
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: September 2, 2008
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

UniProtKB secondary accession numbers

Index of UniProtKB secondary accession numbers

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents